다양한 고정용액에 보존된 고등어(Scomber japonicus) 난의 경과 시간에 따른 난경 변화
Change in the Egg Diameter of Chub Mackerel Scomber japonicus Preserved in Fixing Solution
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We investigated the changes in the egg diameter of chub mackerel Scomber japonicus with the stages of egg development (and distinguished between hydrated oocyte and non-hydrated oocyte) for 1, 2, 3, 5, 10, 15 and 30 days. The chub mackerel oocytes were preserved in seven fixing solutions (70% ethyl alcohol, 99.9% ethyl alcohol, 5% formalin, 10% formalin, 5% neutral buffered formalin, 10% neutral buffered formalin and Gilson’s solution). At 30 days, the chub mackerel hydrated oocytes preserved in 70% ethyl alcohol and 99.9% ethyl alcohol had shrunk by 5.2% and 7.9%, respectively. Similarly, the non-hydrated oocytes in the same solutions shrunk by 10.3% and 14.0%, respectively. Oocytes preserved in Gilson’s solution had an average egg diameter decrease in both the hydrated oocyte (by 16.9%) and non-hydrated oocytes (by 15.6%). The diameter of the preserved hydrated oocytes did not significantly differ between the 5% formalin, 10% formalin, 5% neutral buffered formalin and 10% neutral buffered formalin, with shrinkage percentages of 0.6%, 0.1%, 1.9% and 3.4%, respectively (P>0.05). Similarly, the shrinkage percentages of the non-hydrated oocytes were 4.3% (5% formalin), 5.5% (10% formalin), 4.3% (5% neutral buffered formalin), and 4.1% (10% neutral buffered formalin).
Abstract
We investigated the changes in the egg diameter of chub mackerel Scomber japonicus with the stages of egg development (and distinguished between hydrated oocyte and non-hydrated oocyte) for 1, 2, 3, 5, 10, 15 and 30 days. The chub mackerel oocytes were preserved in seven fixing solutions (70% ethyl alcohol, 99.9% ethyl alcohol, 5% formalin, 10% formalin, 5% neutral buffered formalin, 10% neutral buffered formalin and Gilson’s solution). At 30 days, the chub mackerel hydrated oocytes preserved in 70% ethyl alcohol and 99.9% ethyl alcohol had shrunk by 5.2% and 7.9%, respectively. Similarly, the non-hydrated oocytes in the same solutions shrunk by 10.3% and 14.0%, respectively. Oocytes preserved in Gilson’s solution had an average egg diameter decrease in both the hydrated oocyte (by 16.9%) and non-hydrated oocytes (by 15.6%). The diameter of the preserved hydrated oocytes did not significantly differ between the 5% formalin, 10% formalin, 5% neutral buffered formalin and 10% neutral buffered formalin, with shrinkage percentages of 0.6%, 0.1%, 1.9% and 3.4%, respectively (P>0.05). Similarly, the shrinkage percentages of the non-hydrated oocytes were 4.3% (5% formalin), 5.5% (10% formalin), 4.3% (5% neutral buffered formalin), and 4.1% (10% neutral buffered formalin).
참고문헌 (17)
- Cha HK, "Maturation and spawning of the chub mackerel, Scomber japonicus Houttuyn in Korean waters", J Korean Soc Fish Res, 5, pp.24~33 (2002)
- Dickerson TL, "Spawning frequency and batch fecundity of chub mackerel, Scomber japonicus, during 1985", Cal Coop Ocean Fish Invest Rep, 33, pp.130~140 (1992)
- Finn RN, "In vivo oocyte hydration in Atlantic halibut (Hippoglossus hipoglossus): proteolytic liberation of free amino acids, and ion transport, are driving forces for osmotic water influx", J Exp Biol, 205, pp.211~224 (2002)[DOI]
- Hajibabaei M, "Critical factors for assembling a high volume of DNA barcodes", Phil Trans R Soc B, 360, pp.1959~1967 (2005)[DOI]
- Hislop JRG, "Observations on the size, dry weight and energy content of the eggs of some demersal fish species from British marine waters", J Fish Biol, 31, pp.1~20 (1987)[DOI]
- Hostein I, "Nucleic acid quality preservation by an alcoholbased fixative: comparison with frozen tumors in routine pathology setting", Diagn Mol Pathol, 20, pp.52~62 (2011)[DOI]
- Batch fecundity in multiple spawning fishes, "Hunter JR", 67~77, pp.NOAA Technical Report NMFS 36 (1985)
- Joseph J, "Fecundity of yellowfin tuna (Thunnus albacares) and skipjack (Katsuwonus pelamis) from the eastern Pacific Ocean", Inter-Amer Trop Tuna Com Bull, 7, pp.255~292 (1963)
- 정경미, "한국 연근해 고등어(Scomber japonicus) 알의 비중과 수직분포", 한국수산과학회지, 46(6), pp.957~965 (2013), 한국수산과학회
- Karaiskou N, "Horse mackerel egg identification using DNA methodology", Mar Ecol, 28, pp.429~434 (2007)[DOI]
- Kim SA, "Spawning and early life history of fish", pp.42~80 (1994), Seoul Press
- Klibansky N, "Species-specific effects of four preservative treatments on oocytes and ovarian material of Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), and American plaice (Hippoglossoides platessoides)", Fish Bull, 105, pp.538~547 (2007)
- Lewis LA, "Integrating DNA barcoding of fish eggs into ichthyoplankton monitoring programs", Fish Bull, 114, pp.153~165 (2016)[DOI]
- Lubzens E, "Oogenesis in teleosts: how fish eggs are formed", Gen Comp Endocrinol, 165, pp.367~389 (2010)[DOI]
- Rakka M, "Assessing species and stagespecific effects of preservation on fish oocytes over different temporal scales", Mediterr Mar Sci, 16, pp.533~537 (2015)[DOI]
- Shiraishi T, "Reproductive parameters of the chub mackerel Scomber japonicus estimated from human chorionic gonadotropin-induced final oocyte maturation and ovulation in captivity", Fish Sci, 71, pp.531~542 (2005)[DOI]
- Yamada T, "Spawning time, spawning frequency and fecundity of Japanese chub mackerel, Scomber japonicus in the waters around the Izu Island, Japan", Fish Res, 38, pp.83~89 (1998)[DOI]