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가열-건조처리 넙치(Paralichthys olivaceus) 알 농축물의 식품기능성 및 생리활성

Food Functionality and In Vitro Bioactivity of Olive Flounder Paralichthys olivaceus Roe Concentrates Prepared by Cook-dried Process

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초록

Boil-dried concentrate (BDC) and steam-dried concentrate (SDC) were prepared from olive flounder Paralichthys olivaceus roe using the cook-dried process, and their food functionality and in vitro bioactivity were examined. The buffer capacity of BDC and SDC was found to be stronger in the alkaline region than in the acidic region, and the buffer capacity of SDC was superior to that of BDC. The water holding capacities of these concentrates were 7.6 and 7.4 g/g protein, respectively, both of which were significantly lower than that of freeze-dried concentrate (FDC). The solubility of BDC (13.4%) and SDC (12.7%), foaming capacity of BDC (107.7%) and SDC (110.6%), and oil-in-water emulsifying activity index of BDC (7.7 m2/g) and SDC (9.7 m2/g) were all significantly lower than the corresponding values for FDC (P<0.05). The lower food functionality of BDC and SDC compared with FDC can be attributed to the high-temperature denaturation of proteins during the cook-dried process. The 2,2′-azino-bis-3-ethylbenzothiazoline- 6-sulfonic acid radical scavenging activities (IC50) of SDC (2.5 mg protein/mL) was 60.4 μg/mL, and the angiotensin I converting enzyme inhibitory activity was 80.9%. Olive flounder roe concentrates have good antioxidant and antihypertensive activities, and can be used as materials or ingredients in the processing of seafood and other foods to enhance protein contents and food functionality.

Abstract

Boil-dried concentrate (BDC) and steam-dried concentrate (SDC) were prepared from olive flounder Paralichthys olivaceus roe using the cook-dried process, and their food functionality and in vitro bioactivity were examined. The buffer capacity of BDC and SDC was found to be stronger in the alkaline region than in the acidic region, and the buffer capacity of SDC was superior to that of BDC. The water holding capacities of these concentrates were 7.6 and 7.4 g/g protein, respectively, both of which were significantly lower than that of freeze-dried concentrate (FDC). The solubility of BDC (13.4%) and SDC (12.7%), foaming capacity of BDC (107.7%) and SDC (110.6%), and oil-in-water emulsifying activity index of BDC (7.7 m2/g) and SDC (9.7 m2/g) were all significantly lower than the corresponding values for FDC (P<0.05). The lower food functionality of BDC and SDC compared with FDC can be attributed to the high-temperature denaturation of proteins during the cook-dried process. The 2,2′-azino-bis-3-ethylbenzothiazoline- 6-sulfonic acid radical scavenging activities (IC50) of SDC (2.5 mg protein/mL) was 60.4 μg/mL, and the angiotensin I converting enzyme inhibitory activity was 80.9%. Olive flounder roe concentrates have good antioxidant and antihypertensive activities, and can be used as materials or ingredients in the processing of seafood and other foods to enhance protein contents and food functionality.

참고문헌 (62)

  1. Mohsen Azadian, "Comparison of functional properties and SDS-PAGE patterns between fish protein isolate and surimi produced from silver carp", European Food Research and Technology, 235(1), pp.83~90 (2012), Springer Science and Business Media LLC[DOI]
  2. B. K. Bala, "EXPERIMENTAL INVESTIGATION ON SOLAR DRYING OF FISH USING SOLAR TUNNEL DRYER", Drying Technology, 19(2), pp.427~436 (2001), Informa UK Limited[DOI]
  3. Balaswamy K, "Chemical composition and some functional properties of fish egg(roes)protein concentrate of rohu(Labeo rohita)", J Food Sci and Tech, 44, pp.293~296 (2007)
  4. S. Bellagha, "Drying kinetics and characteristic drying curve of lightly salted sardine (Sardinella aurita)", Drying Technology, 20(7), pp.1527~1538 (2002), Informa UK Limited[DOI]
  5. Asli Can Karaca, "Emulsifying properties of chickpea, faba bean, lentil and pea proteins produced by isoelectric precipitation and salt extraction", Food Research International, 44(9), pp.2742~2750 (2011), Elsevier BV[DOI]
  6. Jang Woo Cha, "Food functionalities and bioactivities of protein isolates recovered from skipjack tuna roe by isoelectric solubilization and precipitation", Food Sci Nutri, 8(4), pp.1874~1887 (2020), Wiley[DOI]
  7. M. Chalamaiah, "Chemical composition and functional properties of mrigal (Cirrhinus mrigala) egg protein concentrates and their application in pasta", Journal of Food Science and Technology, 50(3), pp.514~520 (2011), Springer Science and Business Media LLC[DOI]
  8. M. Chalamaiah, "Chemical composition, molecular mass distribution and antioxidant capacity of rohu (Labeo rohita) roe (egg) protein hydrolysates prepared by gastrointestinal proteases", Food Research International, 52(1), pp.221~229 (2013), Elsevier BV[DOI]
  9. Jean Marc Chobert, "Solubility and emulsifying properties of caseins and whey proteins modified enzymically by trypsin", Journal of Agricultural and Food Chemistry, 36(5), pp.883~892 (1988), American Chemical Society (ACS)[DOI]
  10. Jong-il Choi, "Physiological properties of tuna cooking drip hydrolysate prepared with gamma irradiation", Process Biochemistry, 46(9), pp.1875~1878 (2011), Elsevier BV[DOI]
  11. J.S. Choi, "Physiological properties of Engraulis japonicus muscle protein hydrolysates prepared by subcritical water hydrolysis", Journal of Environmental Biology, 38(2), pp.283~289 (2017), Triveni Enterprises[DOI]
  12. M. Y. Cordero-de-los-Santos, "Physicochemical and functional characterisation of amaranth (Amaranthus hypochondriacus)protein isolates obtained by isoelectric precipitation and micellisation", Food Science and Technology International, 11(4), pp.269~280 (2005), SAGE Publications[DOI]
  13. Damodaran S, "Food Proteins and Their Applications", pp.57~110 (1997), Marcel Dekker
  14. Dickinson E, "Food Macromolecules and Colloids", pp.201~274 (1994), The Royal Society of Chemistry
  15. Zhen-hua Duan, "Drying and quality characteristics of tilapia fish fillets dried with hot air-microwave heating", Food and Bioproducts Processing, 89(4), pp.472~476 (2011), Elsevier BV[DOI]
  16. Galla NR, "Physico-chemical, functional and antioxidant properties of roe protein concentrates from Cyprinus carpio and Epinephelus tauvina", J Food Pharm Sci, 1, pp.81~88 (2013)[DOI]
  17. Narsing Rao Galla, "Chemical and functional characterization of Gum karaya (Sterculia urens L.) seed meal", Food Hydrocolloids, 24(5), pp.479~485 (2010), Elsevier BV[DOI]
  18. Narsing Rao Galla, "Physico-chemical, amino acid composition, functional and antioxidant properties of roe protein concentrates obtained from Channa striatus and Lates calcarifer", Food Chemistry, 132(3), pp.1171~1176 (2012), Elsevier BV[DOI]
  19. Galla NR, "Functional properties and in vitro antioxidant activity of roe protein hydrolysates of Channa striatus and Labeo rohita", Food Chem, 135, pp.1479~1484 (2012)[DOI]
  20. Gbogouri GA, "Influence of hydrolysis degree on the functional properties of salmon byproduct hydrolysates", J Food Sci, 69, pp.615~622 (2004)[DOI]
  21. Peter J. Hailing, "Protein‐stabilized foams and emulsions", C R C Critical Reviews in Food Science and Nutrition, 15(2), pp.155~203 (1981), Informa UK Limited[DOI]
  22. 허민수, "가다랑어 및 황다랑어 알의 식품성분 특성", 한국수산과학회지, 39(1), pp.1~8 (2006), 한국수산과학회
  23. S.W.A. Himaya, "An active peptide purified from gastrointestinal enzyme hydrolysate of Pacific cod skin gelatin attenuates angiotensin-1 converting enzyme (ACE) activity and cellular oxidative stress", Food Chemistry, 132(4), pp.1872~1882 (2012), Elsevier BV[DOI]
  24. Ronny Horax, "Protein extraction optimisation, characterisation, and functionalities of protein isolate from bitter melon (Momordica charantia) seed", Food Chemistry, 124(2), pp.545~550 (2011), Elsevier BV[DOI]
  25. Rossawan Intarasirisawat, "Chemical compositions of the roes from skipjack, tongol and bonito", Food Chemistry, 124(4), pp.1328~1334 (2011), Elsevier BV[DOI]
  26. Rossawan Intarasirisawat, "Isolation of antioxidative and ACE inhibitory peptides from protein hydrolysate of skipjack (Katsuwana pelamis) roe", Journal of Functional Foods, 5(4), pp.1854~1862 (2013), Elsevier BV[DOI]
  27. Won-Kyo Jung, "Angiotensin I-converting enzyme inhibitory peptide from yellowfin sole (Limanda aspera) frame protein and its antihypertensive effect in spontaneously hypertensive rats", Food Chemistry, 94(1), pp.26~32 (2006), Elsevier BV[DOI]
  28. 김형준, "동결건조로 제조한 어류 알 농축물의 식품성분 특성", 한국수산과학회지, 53(2), pp.165~173 (2020), 한국수산과학회[DOI]
  29. 김상보, "Antioxidant and angiotensin I-converting enzyme inhibitory activities of northern shrimp (Pandalus borealis) by-products hydrolysate by enzymatic hydrolysis", Fisheries and Aquatic Sciences, 19(3), pp.1~6 (2016), 한국수산과학회
  30. John E. Kinsella, "Functional properties of proteins in foods: A survey", C R C Critical Reviews in Food Science and Nutrition, 7(3), pp.219~280 (1976), Informa UK Limited[DOI]
  31. Vilailak Klompong, "Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type", Food Chemistry, 102(4), pp.1317~1327 (2007), Elsevier BV[DOI]
  32. Hordur G. Kristinsson, "Fish Protein Hydrolysates: Production, Biochemical, and Functional Properties", Critical Reviews in Food Science and Nutrition, 40(1), pp.43~81 (2000), Informa UK Limited[DOI]
  33. Hordur G. Kristinsson, "A Comparative Study between Acid-and Alkali-aided Processing and Surimi Processing for the Recovery of Proteins from Channel Catfish Muscle", Journal of Food Science, 70(4), pp.C298~C306 (2006), Wiley[DOI]
  34. 이균우, "등전점 용해", 침전 공정으로 어류 알 분리단백질의 제조과정에서 발생하는 가공처리수에 대한 식품기능성 및 생리활성, 2017, pp.50(6), 694~706[DOI]
  35. 이현지, "Preparation and characterization of protein isolate from Yellowfin tuna Thunnus albacares roe by isoelectric solubilization", precipitation process, 2016, pp.19(2), 1~10[DOI]
  36. 이현지, "Chemical composition of protein concentrate prepared from Yellowfin tuna Thunnus albacares roe by cook-dried process", Fisheries and Aquatic Sciences, 19(2), pp.1~8 (2016), 한국수산과학회
  37. Lowry OH, "Protein measurement with the Folin phenol reagent", J Biol Chem, 193, pp.265~275 (1951)
  38. K. Al-Sayed Mahmoud, "Characterisation of the lipid fractions obtained by proteolytic and chemical extractions from rainbow trout (Oncorhynchus mykiss) roe", Process Biochemistry, 43(4), pp.376~383 (2008), Elsevier BV[DOI]
  39. "Yearbook of Marine Resource" (2021)[URL]
  40. Mohamed Beva Kelfala Foh, "Influence of pH Shift on Functional Properties of Protein Isolated of Tilapia (Oreochromis niloticus) Muscles and of Soy Protein Isolate", Food and Bioprocess Technology, 5(6), pp.2192~2200 (2011), Springer Science and Business Media LLC[DOI]
  41. Mukund Mohan, "Functional properties of Rohu (Labeo rohita) proteins during iced storage", Food Research International, 39(8), pp.847~854 (2006), Elsevier BV[DOI]
  42. W.A.M. MUTILANGI, "Functional Properties of Hydrolysates from Proteolysis of Heat‐denatured Whey Protein Isolate", Journal of Food Science, 61(2), pp.270~275 (1996), Wiley[DOI]
  43. Dai-Hung Ngo, "Active peptides from skate (Okamejei kenojei) skin gelatin diminish angiotensin-I converting enzyme activity and intracellular free radical-mediated oxidation", Food Chemistry, 143, pp.246~255 (2014), Elsevier BV[DOI]
  44. 박성환, "Protein functionality of concentrates prepared from yellowfin tuna (Thunnus albacares) roe by cook-dried process", Food Science and Biotechnology, 25(6), pp.1569~1575 (2016), 한국식품과학회[DOI]
  45. C. Pires, "Properties of protein powder prepared from Cape hake by-products", Journal of Food Engineering, 108(2), pp.268~275 (2012), Elsevier BV[DOI]
  46. Subramaniam Sathivel, "Properties of soluble protein powders from Alaska pollock (Theragra chalcogramma)", International Journal of Food Science &amp; Technology, 41(5), pp.520~529 (2006), Wiley[DOI]
  47. SUBRAMANIAM SATHIVEL, "A COMPARISON OF PHYSICAL AND RHEOLOGIC PROPERTIES OF ARROWTOOTH FLOUNDER PROTEIN MADE USING THREE DIFFERENT EXTRACTING PROCESSES", Journal of Food Biochemistry, 32(5), pp.557~575 (2008), Hindawi Limited[DOI]
  48. S. Sathivel, "Biochemical and functional properties of herring (Clupea harengus) by product hydrolysates", Journal of Food Science, 68(7), pp.2196~2200 (2003), Wiley[DOI]
  49. Subramaniam Sathivel, "Properties of protein powders from arrowtooth flounder (Atheresthes stomias) and herring (Clupea harengus) byproducts", Journal of Agricultural and Food Chemistry, 52(16), pp.5040~5046 (2004), American Chemical Society (ACS)[DOI]
  50. Marloes Schurink, "Novel peptides with tyrosinase inhibitory activity", Peptides, 28(3), pp.485~495 (2007), Elsevier BV[DOI]
  51. Zdzislaw E. Sikorski, "Modification of technological properties of fish protein concentrates", C R C Critical Reviews in Food Science and Nutrition, 14(3), pp.201~230 (1981), Informa UK Limited[DOI]
  52. Sikorski ZE, "Seafood Proteins", pp.6~12 (1994), Chapman and Hall
  53. Ee-San Tan, "A comparative study of physicochemical characteristics and functionalities of pinto bean protein isolate (PBPI) against the soybean protein isolate (SPI) after the extraction optimisation", Food Chemistry, 152, pp.447~455 (2014), Elsevier BV[DOI]
  54. Hui-Chun Wu, "Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus)", Food Research International, 36(9-10), pp.949~957 (2003), Elsevier BV[DOI]
  55. 윤인성, "넙치(Paralichthys olivaceus) 및 가다랑어(Katsuwonus pelamis) 알로부터 알칼리 가용화과정을 통해 회수한 알칼리 불용성획분의 이화학적 성분특성", 한국수산과학회지, 51(3), pp.230~237 (2018), 한국수산과학회[DOI]
  56. 윤인성, "동결건조 어류 알 농축물의 식품기능성 및 생리활성", 한국수산과학회지, 53(3), pp.403~416 (2020), 한국수산과학회[DOI]
  57. 윤인성, "어류 알로부터 알칼리 가용화공정을 통해 회수한 Collagenous Protein 획분의 식품 기능특성", 한국수산과학회지, 51(4), pp.351~361 (2018), 한국수산과학회[DOI]
  58. 윤인성, "Fish Roe Concentrates의 제조과정 중에 발생하는 Processed Waters 의 식품기능성과 생리활성", 한국수산과학회지, 50(5), pp.506~519 (2017), 한국수산과학회[DOI]
  59. In Seong Yoon, "Chemical composition and functional properties of roe concentrates from skipjack tuna (Katsuwonus pelamis)by cook-dried process", Food Sci Nutr, 6(5), pp.1276~1286 (2018), Wiley[DOI]
  60. In Seong Yoon, "Food functionality of protein isolates extracted from yellowfin Tuna (Thunnus albacares) roe using alkaline solubilization and acid precipitation process", Food Sci Nutri, 7(2), pp.412~424 (2019), Wiley[DOI]
  61. Lijun You, "Purification and identification of antioxidative peptides from loach (Misgurnus anguillicaudatus) protein hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry", Food Research International, 43(4), pp.1167~1173 (2010), Elsevier BV[DOI]
  62. Boen Yuan, "Effects of limited enzymatic hydrolysis with pepsin and high-pressure homogenization on the functional properties of soybean protein isolate", LWT - Food Science and Technology, 46(2), pp.453~459 (2012), Elsevier BV[DOI]

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넙치(Paralichthys olivaceus) 알로부터 등전점 가용화/침전공정으로 회수한 분리단백질의 식품기능성에 미치는 pH-shift의 영향강상인, 권인상, 김진수, 김형준, 윤인성, 이정석, 허민수한국수산과학회 · 한국수산과학회지2024 · 제57권 제1호동결건조 어류 알 농축물의 식품기능성 및 생리활성강상인, 김도엽, 김진수, 김형준, 윤인성, 이창영, 정우철, 허민수한국수산과학회 · 한국수산과학회지2020 · 제53권 제3호넙치(Paralichthys olivaceus) 알의 증자-건조처리 농축분말로부터 다양 한 효소 가수분해물의 제조 및 식품기능특성강상인, 김민교, 윤인성, 이정석, 허민수한국수산과학회 · 한국수산과학회지2024 · 제57권 제5호가열-건조처리로 제조한 넙치(Paralichthys olivaceus) 알 농축물의 식품 특성강상인, 권인상, 김진수, 김형준, 윤인성, 허민수한국수산과학회 · 한국수산과학회지2022 · 제55권 제6호Fish Roe Concentrates의 제조과정 중에 발생하는 Processed Waters 의 식품기능성과 생리활성강상인, 김진수, 박선영, 윤인성, 이균우, 허민수한국수산과학회 · 한국수산과학회지2017 · 제50권 제5호넙치(Paralichthys olivaceus) 알의 분리단백질로부터 제조한 효소 가수 분해물의 식품기능 특성 및 생리활성강상인, 김민교, 윤인성, 허민수한국수산과학회 · 한국수산과학회지2025 · 제58권 제1호

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