홍조 털지누아리(Polyopes lancifolius)의 크러스트 배양에서 직립엽체 형 성에 미치는 온도와 광량 영향
Effect of Temperature and Irradiance on Upright Thalli Formation in the Crust Culture of Polyopes lancifolius (Rhodophyta)
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The red alga Polyopes lancifolius has traditionally been used in jelly-based foods. We investigated how to optimize indoor cultivation conditions during the seedling crust stage to support their transition to upright thalli formation. Experiments were conducted under five temperatures (10, 14, 18, 22 and 26°C) and three irradiance settings (20, 50 and 80 μmol m-²s-¹). The highest growth rate of the crusts was observed at 26°C and 20 μmol m-²s-¹, achieving 8.7% day-¹. At 22°C, the growth rate increased as irradiance decreased (20 >50 >80 μmol m-²s-¹). The density of upright thalli was most significant at 14°C and 80 μmol m-²s-¹, with 31.3±5.0 individuals mm-² (≥100 μm in length). At 14°C, thalli formation density improved with higher irradiance (80>50 >20 μmol m-²s-¹). During the first 10 weeks, Polyopes adapted to the high-temperature and low-irradiance conditions in the crust stage. After this period, the cultivation environment should be shifted to low-temperature and high-irradiance conditions to facilitate the growth of upright thalli. Lowering the temperature to 14–18°C and increasing the irradiance to over 60 μmol m-²s-¹ will promote upright thalli formation and ensure optimal growth.
Abstract
The red alga Polyopes lancifolius has traditionally been used in jelly-based foods. We investigated how to optimize indoor cultivation conditions during the seedling crust stage to support their transition to upright thalli formation. Experiments were conducted under five temperatures (10, 14, 18, 22 and 26°C) and three irradiance settings (20, 50 and 80 μmol m-²s-¹). The highest growth rate of the crusts was observed at 26°C and 20 μmol m-²s-¹, achieving 8.7% day-¹. At 22°C, the growth rate increased as irradiance decreased (20 >50 >80 μmol m-²s-¹). The density of upright thalli was most significant at 14°C and 80 μmol m-²s-¹, with 31.3±5.0 individuals mm-² (≥100 μm in length). At 14°C, thalli formation density improved with higher irradiance (80>50 >20 μmol m-²s-¹). During the first 10 weeks, Polyopes adapted to the high-temperature and low-irradiance conditions in the crust stage. After this period, the cultivation environment should be shifted to low-temperature and high-irradiance conditions to facilitate the growth of upright thalli. Lowering the temperature to 14–18°C and increasing the irradiance to over 60 μmol m-²s-¹ will promote upright thalli formation and ensure optimal growth.
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