Comparison of fucosterol content in algae using high-performance liquid chromatography
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Fucosterol is a compound commonly found in algae that has various biological activities. The purpose of this study was to develop a high-performance liquid chromatography (HPLC) validation method for fucosterol and to compare the fucosterol contents of 11 algal species from Ulleungdo, Korea. Method: In this study, we successfully isolated and identified fucosterol from a 70% EtOH extract of Sargassum miyabei, and subsequently conducted specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision analyses for development of an HPLC validation method. Fucosterol contents were compared using the established HPLC validation conditions. Results: We successfully isolated fucosterol from a 70% EtOH extract of S. miyabei and identified it based on spectroscopic analysis. On the basis of HPLC validation using the fucosterol isolated from S. miyabei, we confirmed specificity (8.5 min), linearity (R2 = 0.9998), LOD (3.20 μg mL−1), LOQ (9.77 μg mL−1), accuracy (intra-day and inter-day variation, 90–110%), and precision (RSD, 1.07%). Fucosterol contents in the 11 assessed algal species ranged from 0.22 to 81.67 mg g−1, with the highest content being recorded in a 70% EtOH extract of Desmarestia tabacoides (81.67 mg g−1), followed by that of Agarum clathratum (78.70 mg g−1). Conclusions: The results indicate that 70% EtOH extracts of D. tabacoides and A. clathratum containing fucosterol with various effects can be potential alternative sources of fucosterol.
Abstract
Background: Fucosterol is a compound commonly found in algae that has various biological activities. The purpose of this study was to develop a high-performance liquid chromatography (HPLC) validation method for fucosterol and to compare the fucosterol contents of 11 algal species from Ulleungdo, Korea. Method: In this study, we successfully isolated and identified fucosterol from a 70% EtOH extract of Sargassum miyabei, and subsequently conducted specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, and precision analyses for development of an HPLC validation method. Fucosterol contents were compared using the established HPLC validation conditions. Results: We successfully isolated fucosterol from a 70% EtOH extract of S. miyabei and identified it based on spectroscopic analysis. On the basis of HPLC validation using the fucosterol isolated from S. miyabei, we confirmed specificity (8.5 min), linearity (R2 = 0.9998), LOD (3.20 μg mL−1), LOQ (9.77 μg mL−1), accuracy (intra-day and inter-day variation, 90–110%), and precision (RSD, 1.07%). Fucosterol contents in the 11 assessed algal species ranged from 0.22 to 81.67 mg g−1, with the highest content being recorded in a 70% EtOH extract of Desmarestia tabacoides (81.67 mg g−1), followed by that of Agarum clathratum (78.70 mg g−1). Conclusions: The results indicate that 70% EtOH extracts of D. tabacoides and A. clathratum containing fucosterol with various effects can be potential alternative sources of fucosterol.
참고문헌 (22)
- 방면호, "Anti-osteoporotic Activities of Fucosterol from Sea Mustard (Undaria pinnatifida)", Food Science and Biotechnology, 20(2), pp.343~347 (2011), 한국식품과학회
- Devi GK, "In vitro antioxidant activities of selected seaweeds from Southeast coast of India", Asian Pac J Trop Med, 4, pp.205~211 (2011)
- Guerra Dore CM, "Antiangiogenic activity and direct antitumor effect from a sulfated polysaccharide isolated from seaweed", Microvasc Res, 88, pp.12~18 (2013)
- Ham YM, "Chemical constituents from Sargassum micracanthum and antioxidant activity", Int J Pharmacol, 6, pp.147~151 (2010)
- Hosokawa M, "Fucoxanthin induces apoptosis and enhances the antiproliferative effect of the PPAR γligand, troglitazone, on colon cancer cells", Biochim Biophys Acta, 1675, pp.113~119 (2004)
- Ikeda I, "Inhibition of cholesterol absorption in rats by plant sterols", J Lipid Res, 29, pp.1573~1582 (1988)
- "topic Q2A: Text on validation of analytical procedures. US FDA Federal Register. R1. Validation of analytical procedures: text and methodology", International conference on harmonization (ICH) of technical requirements for registration of pharmaceuticals for human use, 60, pp.11260~11266 (2005)
- "Q2B: Validation of analytical procedures-methodology", International conference on harmonization, 62, pp.27463~27467 (1997), US FDA Federal Register
- 전영은, "해조류의 항산화 활성 및 아세틸콜린에스테라제 저해 활성", 한국식품영양과학회지, 41(4), pp.443~449 (2012), 한국식품영양과학회[DOI]
- Lee DS, "Polyopes affinis alleviates airway inflammation in a murine model of allergic asthma", J Biosci, 36, pp.869~877 (2011)
- Lee YS, "Anti-diabetic activities of fucosterol from Pelvetia siliquosa", Arch Pharm Res, 27, pp.1120~1122 (2004)
- Majik MS, "Isolation of stigmast-5, 24-dien-3-ol from marine brown algae Sargassum tenerrimum and its antipredatory activity", RSC Adv, 5, pp.51008~51011 (2015)
- Meenakshi S, "In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar", Asian Pac J Trop Biomed, 1, pp.S66~S70 (2011)
- Perumal P, "Isolation, structural elucidation and antiplasmodial activity of fucosterol compound from brown seaweed, Sargassum linearifolium against malarial parasite Plasmodium falciparum", Nat Prod Res, 32, pp.1316~1319 (2018)
- Shanmugam N, "Biosynthesis of silver nanoparticles from the marine seaweed Sargassum wightii and their antibacterial activity against some human pathogens", Appl Nanosci, 4, pp.881~888 (2014)
- Souza BWS, "Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae", Food Hydrocolloid, 27, pp.287~292 (2012)
- Synytsya A, "Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida", Carbohydr Polym, 81, pp.41~48 (2010)
- Tang HF, "Bioactive steroids from the brown alga Sargassum carpophyllum", J Asian Nat Prod Res, 4, pp.95~101 (2002)
- Terasaki M, "Evaluation of recoverable functional lipid components of several brown seaweeds(Phaeophyta)from Japan with special reference to fucoxanthin and fucosterol contents", J Phycol, 45, pp.974~980 (2009)
- Thuy TT, "Anti-HIV activity of fucoidans from three brown seaweed species", Carbohydr Polym, 115, pp.122~128 (2015)
- Veiga-Santos P, "In vitro antitrypanosomal activity of elatol isolated from red seaweed Laurencia dendroidea", Parasitology, 137, pp.1661~1670 (2010)
- Zhen XH, "Fucosterol, a sterol extracted from Sargassum fusiforme, shows antidepressant and anticonvulsant effects", Eur J Pharmacol, 768, pp.131~138 (2015)