본문 바로가기
KERD
논문KCI 등재

Effect of different temperatures on GH-transgenesis, testosterone levels and spermatozoa density of G5 transgenic mutiara catfish (Clarias gariepinus)

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

Unstable changes in environmental temperature (during the dry season there are several times of rain) can damage the fish reproductive cycle, leading to inhibition of spawning activity. Low temperatures cause slow fish gonad maturation, while high temperatures cause delays in the spermatogenesis process of male fish. The ideal temperature range can increase fish testosterone (T) levels needed to maintain spermatogenesis and GH expression levels during sperm cell proliferation. The effect of temperature on the presence of exogenous GH (CgGH) in G5 transgenic mutiara catfish formed through broodstock breeding GH-transgenesis technology in previous research needs to be evaluated for testicular fertility, especially T levels and spermatozoa (Spz) density during fish gonad maturation. The results showed that the ideal temperature that increases T levels, Spz density and CgGH expression levels in G5 transgenic mutiara catfish is 24℃. Temperatures of 28℃ and 32℃ tended to reduce T levels and Spz density in both transgenic and nontransgenic catfish. Besides that, the sperm maturation stage (formation of Spz) was more dominantly induced by 24℃ compared to other temperature treatments. This indication shows that the maximum enzyme activity involved in T production to drive the spermatogenesis process is regulated at 24℃. As a consequence, the use of transgenic male fish broodstock (transgenesis-GH) and temperature regulation for male fish gonad maturation is more profitable in the range of 24℃–28℃.

Abstract

Unstable changes in environmental temperature (during the dry season there are several times of rain) can damage the fish reproductive cycle, leading to inhibition of spawning activity. Low temperatures cause slow fish gonad maturation, while high temperatures cause delays in the spermatogenesis process of male fish. The ideal temperature range can increase fish testosterone (T) levels needed to maintain spermatogenesis and GH expression levels during sperm cell proliferation. The effect of temperature on the presence of exogenous GH (CgGH) in G5 transgenic mutiara catfish formed through broodstock breeding GH-transgenesis technology in previous research needs to be evaluated for testicular fertility, especially T levels and spermatozoa (Spz) density during fish gonad maturation. The results showed that the ideal temperature that increases T levels, Spz density and CgGH expression levels in G5 transgenic mutiara catfish is 24℃. Temperatures of 28℃ and 32℃ tended to reduce T levels and Spz density in both transgenic and nontransgenic catfish. Besides that, the sperm maturation stage (formation of Spz) was more dominantly induced by 24℃ compared to other temperature treatments. This indication shows that the maximum enzyme activity involved in T production to drive the spermatogenesis process is regulated at 24℃. As a consequence, the use of transgenic male fish broodstock (transgenesis-GH) and temperature regulation for male fish gonad maturation is more profitable in the range of 24℃–28℃.

참고문헌 (34)

  1. Baroiller JF, "Oestrogen and insulin-like growth factors during the reproduction and growth of the tilapia Oreochromis niloticus and their interactions", Gen Comp Endocrinol, 205, pp.142~150 (2014)
  2. Buwono ID, "CgGH and IGF-1 expression level and growth response of G4 transgenic mutiara strain catfish(Clarias gariepinus)reared at different stocking densities", Aquac Int, 31, pp.827~846 (2023)
  3. Buwono ID, "Growth hormone transgenesis and feed composition influence growth and protein and amino acid content in transgenic G3 mutiara catfish(Clarias gariepinus)", Aquac Int, 29, pp.431~451 (2021)
  4. Buwono ID, "Reproduction performance of transgenic mutiara catfish(G1)comprising the growth hormone gene", J Biotech Res, 10, pp.199~212 (2019)
  5. Buwono ID, "GnRHr, LHr, and Vg gene expression levels and ovarian development of G5 transgenic mutiara female catfish(Clarias gariepinus)after exposure photoperiod induction", Discover Appl Sci, 6, pp.63~ (2024)
  6. de Alvarenga ÉR, "Effects of different temperatures on testis structure and function, with emphasis on somatic cells, in sexually mature Nile tilapias(Oreochromis niloticus)", Biol Reprod, 80, pp.537~544 (2009)
  7. Hallerman EM, "Effects of growth hormone transgenes on the behavior and welfare of aquacultured fishes : a review identifying research needs", Appl Anim Behav Sci, 104, pp.265~294 (2007)
  8. Harvey BJ, "The theory and practise of induced breeding in fish" (1979), International Development Research Centure
  9. Kime DE, "The effect of temperature and gonadotropin on testicular steroidogenesis in Sarotherodon(Tilapia)mossambicus in vitro", Gen Comp Endocrinol, 50, pp.105~115 (1983)
  10. Le Gac F, "Evidence for binding and action of growth hormone in trout testis", Biol Reprod, 46, pp.949~957 (1992)
  11. Maestro MA, "Ovarian receptors for insulin and insulin-like growth factor I(IGF-I)and effects of IGF-I on steroid production by isolated follicular layers of the preovulatory coho salmon ovarian follicle", Gen Comp Endocrinol, 106, pp.189~201 (1997)
  12. Mañanós E, "Methods in reproductive aquaculture: marine and freshwater species", pp.3~66 (2008), CRC Press
  13. Melo RMC, "Effects of temperature manipulation on gamete development and reproductive activity in the farmed catfish Lophiosilurus alexandri", Anim Reprod Sci, 247, pp.107100~ (2022)
  14. Miura C, "Gh is produced by the testis of Japanese eel and stimulates proliferation of spermatogonia", Reprod Res, 142, pp.869~877 (2011)
  15. Nam YK, "Generation of transgenic homozygous line carrying the CAT gene in mud loach Misgurnus mizolepis", Fish Sci, 66, pp.58~62 (2000)
  16. nee Pathak ND, "Endocrine regulation of testosterone production by Leydig cells in the catfish, Clarias batrachus : probable mediators of growth hormone", Anim Reprod Sci, 154, pp.158~165 (2015)
  17. Negatu Z, "Effects of insulin-like growth factor-I on final oocyte maturation and steroid production in Fundulus heteroclitus", Fish Physiol Biochem, 19, pp.13~21 (1998)
  18. Nóbrega RH, "Fsh stimulates spermatogonial proliferation and differentiation in zebrafish via Igf3", Endocrinology, 156, pp.3804~3817 (2015)
  19. Okoye CN, "Ovarian morphology and development of 3 to 8 months old African catfish, Clarias gariepinus", Comp Clin Pathol, 27, pp.887~891 (2018)
  20. Peñaranda DS, "Temperature modulates testis steroidogenesis in European eel", Comp Biochem Physiol A Mol Integr Physiol, 197, pp.58~67 (2016)
  21. Pfaffl MW, "A new mathematical model for relative quantification in real-time RT–PCR", Nucleic Acids Res, 29, pp.2002~2007 (2001)
  22. Quirino PP, "The influence of increased water temperature on the duration of spermatogenesis in a neotropical fish, Astyanax altiparanae(Characiformes, Characidae)", Fish Physiol Biochem, 47, pp.747~755 (2021)
  23. Raghuveer K, "Cloning and differential expression pattern of vasa in the developing and recrudescing gonads of catfish, Clarias gariepinus", Comp Biochem Physiol A Mol Integr Physiol, 157, pp.79~85 (2010)
  24. Schmittgen T, "Analyzing real-time PCR data by the comparative CT method", Nat Protoc, 3, pp.1101~1108 (2001)
  25. Schulz RW, "Spermatogenesis in fish", Gen Comp Endocrinol, 165, pp.390~411 (2010)
  26. Schulz RW, "Sertoli cell proliferation in the adult testis is induced by unilateral gonadectomy in African catfish", Gen Comp Endocrinol, 177, pp.160~167 (2012)
  27. Scott AP, "The role of the maturation-inducing steroid, 17, 20β-dihydroxypregn-4-en-3-one, in male fishes : a review", J Fish Biol, 76, pp.183~224 (2010)
  28. Reproductive physiology of teleost fishes: a review of present knowledge and needs for future research, "Sundararaj BI", Food and Agriculture Organization of the United Nations, ~ (1981)
  29. Taranger GL, "Control of puberty in farmed fish", Gen Comp Endocrinol, 165, pp.483~515 (2010)
  30. Tokalov SV, "Spermatogenesis in testis primary cell cultures of the tilapia(Oreochromis niloticus)", Dev Dyn, 233, pp.1238~1247 (2005)
  31. Uh M, "Transgene constructs in coho salmon(Oncorhynchus kisutch)are repeated in a head-to-tail fashion and can be integrated adjacent to horizontally-transmitted parasite DNA", Trans Res, 15, pp.711~727 (2006)
  32. Uzbekova S, "Analysis of cell-specificity and variegation of transgene expression driven by salmon prolactin promoter in stable lines of transgenic rainbow trout", Trans Res, 12, pp.213~227 (2003)
  33. Yamaguchi S, "Influence of estradiol-17β, testosterone, and 11-ketotestosterone on testicular development, serum steroid hormone, and gonadotropin secretion in male red sea bream Pagrus major", Fish Sci, 72, pp.835~845 (2006)
  34. Zhang MQ, "Cloning and sequence analysis of full-length growth hormone cDNA from Clarias gariepinus", ACTA Agric Bor Sin, 24, pp.27~32 (2009)

관련 논문AI추천

이 간행물의 다른 논문 (5건)