Poly-P-forming characteristics found in poly- P-accumulating microorganisms (phosphateaccumulating organisms [PAOs]) isolated from shrimp ponds in Vietnam
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Significant amounts of phosphate (PO4 3–) in extensive shrimp ponds make an ideal survey for studying the PO4 3–-accumulating properties of phosphate-accumulating organisms (PAOs) and harvesting polyphosphate (poly-P). In this study, we evaluated the PO4 3–-accumulating capacities of four PAOs (A1, A2, A3, and A5) from those ponds by determining the residual PO4 3–-content in acetate mineral mediums (AMM) with/without NaCl at different inoculated periods. Strain A2 was identified as a Rhodotorula sp., whereas strain A1 was Acinetobacter guillouiae, A3 was Acinetobacter lwoffii, and A5 was Lactococcus raffinolactic. Among four PAOs, strains A1 and A5 showed the highest PO4 3–-accumulating capacities of 338.44 and 333.63 μg in 100 mL of AMM, respectively, after 72 hours under cultural AMM without adding NaCl. All PAOs might extend the inoculation periods to 120 hours for mostly PO4 3–-accumulations in the AMM with NaCl contents (5%–30‰) (p < 0.05). The PO4 3–-accumulation levels from 338.40 to 285.55 μg of Acinetobacter guillotine demonstrated significantly higher capacities than the remaining PAOs (p < 0.05), whereas the maximum PO4 3–-collections of L. raffinolactic with 281.65 μg/100 mL recognized at single condition of NaCl content of 20‰, followed with 279.70 μg/100 mL by A. lwoffii at a NaCl content of 10‰. Upon the rate of PO4 3–-accumulation correlated with growths in the LB medium with different salinities combined, the activities of the four PAOs did not alter the pH of the AMM, around 6.5 to 7.0, providing evidence for the impressing PO4 3–-accumulating capacities of four PAO strains on different survival media conditions. This study is the first report on the accumulation of PO4 3– to forming polyphosphate (poly-P) chains in L. raffinolactic cells; the other strains poly-P granules generated inside cells. Remarkably, our findings comprehensively discovered Günthe forming process of poly-P in A. guillotine cells through image analysis at three inoculation times (0, 24, and 72 hours). These PAOs are viable probiotics that bring advantages in lowering abundant PO4 3–-compounds in aquaculture wastewater and treating different PO4 3–-polluted water resources. The poly-P in PAO cells promises valuable sources for harvesting poly-P applied in the fertilizer industry or biominerals.
Abstract
Significant amounts of phosphate (PO4 3–) in extensive shrimp ponds make an ideal survey for studying the PO4 3–-accumulating properties of phosphate-accumulating organisms (PAOs) and harvesting polyphosphate (poly-P). In this study, we evaluated the PO4 3–-accumulating capacities of four PAOs (A1, A2, A3, and A5) from those ponds by determining the residual PO4 3–-content in acetate mineral mediums (AMM) with/without NaCl at different inoculated periods. Strain A2 was identified as a Rhodotorula sp., whereas strain A1 was Acinetobacter guillouiae, A3 was Acinetobacter lwoffii, and A5 was Lactococcus raffinolactic. Among four PAOs, strains A1 and A5 showed the highest PO4 3–-accumulating capacities of 338.44 and 333.63 μg in 100 mL of AMM, respectively, after 72 hours under cultural AMM without adding NaCl. All PAOs might extend the inoculation periods to 120 hours for mostly PO4 3–-accumulations in the AMM with NaCl contents (5%–30‰) (p < 0.05). The PO4 3–-accumulation levels from 338.40 to 285.55 μg of Acinetobacter guillotine demonstrated significantly higher capacities than the remaining PAOs (p < 0.05), whereas the maximum PO4 3–-collections of L. raffinolactic with 281.65 μg/100 mL recognized at single condition of NaCl content of 20‰, followed with 279.70 μg/100 mL by A. lwoffii at a NaCl content of 10‰. Upon the rate of PO4 3–-accumulation correlated with growths in the LB medium with different salinities combined, the activities of the four PAOs did not alter the pH of the AMM, around 6.5 to 7.0, providing evidence for the impressing PO4 3–-accumulating capacities of four PAO strains on different survival media conditions. This study is the first report on the accumulation of PO4 3– to forming polyphosphate (poly-P) chains in L. raffinolactic cells; the other strains poly-P granules generated inside cells. Remarkably, our findings comprehensively discovered Günthe forming process of poly-P in A. guillotine cells through image analysis at three inoculation times (0, 24, and 72 hours). These PAOs are viable probiotics that bring advantages in lowering abundant PO4 3–-compounds in aquaculture wastewater and treating different PO4 3–-polluted water resources. The poly-P in PAO cells promises valuable sources for harvesting poly-P applied in the fertilizer industry or biominerals.
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