치경부 5급 와동 수복물의 표면 거칠기와 미세누출에 관한 연구: 표면 전색의 효과
Surface roughness and microleakage of class V composite restorations: Effect of surface sealing
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수복물의 변연 미세 누출과 마모 개선을 위해 표면 전색제가 개발되었으며 이의 효과에 대해 알아보기 위해, 규격화된 치경부 5급 와동을 25개의 소구치의 협면에 형성하고 Single Bond와 Z-250으로 수복한 뒤, 37℃의 증류수에 7일간 보관한 후 무작위로 5군으로 나누고 다음과 같은 재료로 제조사의 지시에 따라 표면 처리하였다 : No sealing ; Single Bond Adhesive ; Biscover ; Fortify ; Optiguard. 그 후 잇솔질 마모시험을 시행하였다. 표면 조도는 잇솔질 전후에profilometer로 측정되었고 paired t-test와ANOVA로 비교하였다. 접착면과 표면변화는 전자현미경으로 관찰하였다. 미세누출 평가를 위해 시편은 2% methylene blue 용액으로 염색한 뒤, 협설로 잘라 제작하였다. 입체현미경을 이용하여 교합면쪽과 치경부쪽 계면에서 미세누출을 관찰하고 Kruskal-Wallis와 Mann-Whitney U test를 이용하여 그 결과를 비교하였다. 표면 조도는 잇솔질 후 수치가 증가하였으나 통계학적으로 유의한 차이를 나타내지는 않았다. 전자 현미경 관찰 결과, 표면 전색제가 접착면에 부분적으로 남아있기도 하였으나 많은 부분에서 떨어져 나간 상태였다. 특히, 미세 틈새가 치경부쪽 변연에서 나타났다. 미세누출 실험에서는 법랑질에서 더 봉쇄효과가 좋았으며 교합면쪽 변연에서 군간에 유의할만한 차이를 나타내었다. 조절군과 Single Bond를 도포한 군에서는 치경부쪽 변연보다 법랑질쪽 변연에서 유의하게 좋은 변연 봉쇄 효과를 나타내었다.
Abstract
The purpose of this study was to compare the effect of surface sealing materials on microleakage and surface roughness in Class Ⅴ composite restorations. Twenty five standardized Class Ⅴ cavity preparations were made on the facial surface of human premolars and were randomly assigned to 5 groups. The teeth were restored with Z-250 after applying Single Bond. Following 7 days storage in distilled water at 37℃, the restorations were sealed as following systems : No sealing ; Single Bond Adhesive ; Biscover ; Fortify ; Optiguard. Then, toothbrush abrasion test was conducted using a wear testing machine. Surface roughness was measured by means of profilometer before and after toothbrushing and the results were statistically analysed by using a paired t-test and ANOVA. The bonded interfaces and the changes of surface roughness were examined by SEM. For microleakage test, specimens were stained in a 2% methylene blue solution, then longitudinally sectioned and analyzed for leakage at occlusal and cervical interfaces using stereomicroscope. The results were statistically analysed by using a Kruskal-Wallis and Mann-Whitney U test. Surface roughness was increasing in all groups after toothbrushing, but no statistically significant differences. In SEM observation, surface sealant was partially retained and partially detached in bonded interfaces. Especially, microgap was identified in cervical margins. In microleakage test, there was better seal in the enamel region and a significant difference between groups at occlusal margin. Control group and Single Bond group had significantly better marginal seal at enamel margin than cervical margin.
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