Prevotella nigrescens의 용혈특성에 관한 연구
A study on the hemolytic properties of Prevotella nigrescens
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세균의 용혈활성은 세균이 숙주 내에서 생존하기 위해 필요한 철을 획득하기위한 특성이며 기능면에서 볼 때 숙주에 대한 중요한 독력인자로 간주될 수 있다. 본 연구에서는 괴사치수 및 치근단 치주염으로 진단된 환자의 근관에서 분리한 Prevotella nigrescens의 용혈활성 을 다양한 조건 하에서 측정하여 그 특성을 규명하는 것을 목적으로 한다. 1. 임상에서 분리한 P. nigrescens 와 표준균주인 P. nigrescens ATCC 33563에서 모두용혈활성이 나타났다. 2. 사람, 면양 및 말 세 가지 종에 대해 용혈활성을 비교한 결과 사람의 적혈구에서 가장 강한 용혈활성을 나타내었다. 3. 용혈소 억제제인 NaN3와 dithiothreitol (DTT)는 농도의존적으로 P. nigrescens의 용혈활성을 감소시켰다 (p<0.05). 4. P. nigrescens가 최대 용혈활성을 나타내는 최적의 pH는 4 이었으며, 50oC 이하의 온도에서는 용혈활성을 보였으나 95oC에서 급격히 감소하였다. 5. 배양조건에 따른 P. nigrescens의 용혈활성을 비교한 결과 10％ CO2 배양기에 배양한 경우 혐기성 조건에서 배양한 것보다 더 높은 용혈활성을 보였다.
Abstract
Hemolytic property is a specific feature of bacteria to obtain iron which is essential for its survival in host tissues. Therefore, it is thought to be one of several factors of virulence. The purpose of this study was to investigate the hemolytic properties of Prevotella nigrescens isolated from the teeth diagnosed as pulp necrosis and apical periodontitis under the presence of hemolysin inhibitors such as NaN3 and dithiothreitol, heat, various pH and cultural conditions. The results were as follows; 1. Clinically isolated P. nigrescens strains and standard P. nigrscens ATCC 33563 showed hemolytic activity. 2. P. nigrescens showed higher hemolytic activity against human erythrocytes than sheep or horse erythrocytes. 3. NaN3 and dithiothreitol (DTT) reduced the hemolytic activity of P. nigrescens in a dose dependent manner (p<0.05). 4. Optimal pH for the maximum hemolytic activity of P. nigrescens was 4.0 and the hemolysin was stable under the 50oC, but the hemolytic activity was significantly decreased at 95℃. 5. P. nigrescens cultured in 10% CO2 condition showed higher hemolytic activity than the bacteria cultured in the anaerobic condition.
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