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신개발 인산칼슘계 근관 봉함재의 세포독성 및 유전독성에 관한 연구

Cytotoxicity and genotoxicity of newly developed calcium phosphate-based root canal sealers

백승호|김희정|배광식
대한치과보존학회 (Korean Academy Of Conservative Dentistry)|2006.01|Restorative Dentistry and Endodontics|제31권 제1호|pp. 36-49

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참고문헌

키워드

cytotoxicitygenotoxicity세포독성인산칼슘calcium phosphate생체적합성root canal sealer근관 봉함재유전독성
주제분류치의학

초록

신개발 인산칼슘계 근관 봉함재의 세포독성 및 유전독성에 관한 연구

Abstract

The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus), zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III). According to this study, the results were as follows:The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p < 0.001). CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, Sealapex and SARCS II in freshly mixed group (p < 0.01).AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix.Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix.Among those of 24 h set groups, the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix.No mutagenic effect of CAPSEAL I and CAPSEAL II was detected.There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.

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