쥐의 척수강 내로 투여한 고려 홍삼의 항통각효과에 대한 아드레날린성 및 콜린성 수용체 역할
The Role of Adrenergic and Cholinergic Receptors on the Antinociception of Korean Red Ginseng in the Spinal Cord of Rats
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
Background: Experimental evidence indicates that ginseng modulate the nociceptive transmission. Authors examined the role of adrenergic and cholinergic receptors on the antinociceptive action of Korean red ginseng against the formalin-induced pain at the spinal level. Methods: Catheters were inserted into the intrathecal space of male Sprague-Dawley rats. Fiftyμl of 5% formalin solution was injected to the hindpaw for induction of pain and formalin-induced pain (flinching response) was observed. The role of spinal adrenergic and cholinergic receptors on the effect of Korean red ginseng was assessed by antagonists (prazosin, yohimbine, atropine and mecamylamine). Results: Intrathecal Korean red ginseng produced a dose-dependent suppression of the flinching response in the rat formalin test. All of prazosin, yohimbine, atropine and mecamylamine antagonized the antinociception of Korean red ginseng. Conclusions: Spinal Korean red ginseng is effective against acute pain and facilitated pain state evoked by formalin injection. All of alpha 1, alpha 2, muscarinic and nicotinic receptors may play an important role in the antinociceptive action of Korean red ginseng at the spinal level. (Korean J Pain 2008; 21: 27−32)
Abstract
Background: Experimental evidence indicates that ginseng modulate the nociceptive transmission. Authors examined the role of adrenergic and cholinergic receptors on the antinociceptive action of Korean red ginseng against the formalin-induced pain at the spinal level. Methods: Catheters were inserted into the intrathecal space of male Sprague-Dawley rats. Fiftyμl of 5% formalin solution was injected to the hindpaw for induction of pain and formalin-induced pain (flinching response) was observed. The role of spinal adrenergic and cholinergic receptors on the effect of Korean red ginseng was assessed by antagonists (prazosin, yohimbine, atropine and mecamylamine). Results: Intrathecal Korean red ginseng produced a dose-dependent suppression of the flinching response in the rat formalin test. All of prazosin, yohimbine, atropine and mecamylamine antagonized the antinociception of Korean red ginseng. Conclusions: Spinal Korean red ginseng is effective against acute pain and facilitated pain state evoked by formalin injection. All of alpha 1, alpha 2, muscarinic and nicotinic receptors may play an important role in the antinociceptive action of Korean red ginseng at the spinal level. (Korean J Pain 2008; 21: 27−32)
참고문헌 (16)
- Liu CX, "Recent advances on ginseng research in China", J Ethnopharmacol, 36, pp.27~38 (1992)
- Mogil JS, "Ginsenoside Rf, a trace component of ginseng root, produces antinociception in mice", Brain Res, 792, pp.218~28 (1998)
- Shin YH, "Ginsenosides that produce differential antinociception in mice", Gen Pharmacol, 32, pp.653~9 (1999)
- Yoon SR, "Ginsenosides induce differential antinociception and inhibit substance P induced-nociceptive response in mice", Life Sci, 62, pp.319~25 (1998)
- Bouchenafa O, "Autoradiographic localisation of alpha 2 adrenoceptor binding sites in the spinal cord of the sheep", Res Vet Sci, 42, pp.382~6 (1987)
- quinuclidinylbenzilate binding sites, "Brain Res", 1985, 196~9
- Buerkle H, "Thermal and mechanical antinociceptive action spinal vs peripherally administered clonidine in the rat inflamed knee joint model", Br J Anaesth, 83, pp.436~41 (1999)
- Yoon MH, "Antinociception of intrathecal cholinesterase inhibitors and cholinergic receptors in rats", Acta Anaesthesiol Scand, 47, pp.1079~84 (2003)
- Sala F, "Effects of ginsenoside Rg2 on human neuronal nicotinic acetylcholine receptors", J Pharmacol Exp Ther, 301, pp.1052~9 (2002)
- Tachikawa E, "Proof of the mysterious efficacy of ginseng: basic and clinical trials: suppression of adrenal medullary function in vitro by ginseng", J Pharmacol Sci, 95, pp.140~4 (2004)
- Seung-Yeol Nah, "Ginsenosides Regulate Ligand-gated Ion Channels from the Outside", 분자와 세포, 18(1), pp.115~121 (2004), 한국분자세포생물학회
- Yaksh TL, "Chronic catheterization of the spinal subarachnoid space", Physiol Behav, 17, pp.1031~6 (1976)
- Yoon MH, "Analysis of interactions between serotonin and gabapentin or adenosine in the spinal cord of rats", Pharmacology, 74, pp.15~22 (2005)
- Dickenson AH, "Subcutaneous formalin-induced activity of dorsal horn neurones in the rat: differential response to an intrathecal opiate administered pre or postformalin", Pain, 30, pp.349~60 (1987)
- Wilcox GL, "Pharmacology of spinal afferent processing. In: Anesthesia: biologic foundations", pp.557~76 (1998), Lippincott-Raven Publishers
- Kaku T, "Chemico-pharmacological studies on saponins of Panax ginseng C. A. Meyer. II. Pharmacological part", Arzneimittelforschung, 25, pp.539~47 (1975)