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치주인대세포에 대한 Bone morphogenetic protein-7의 영향

Effect of BMP-7 on the rat periodontal ligament cell

김경희|김영준|정현주
대한치주과학회 (The Korean Academy Of Periodontology)|2005.06|Journal of Periodontal & Implant Science|제35권 제2호|pp. 289-298

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주제분류치의학

초록

Abstract

Bone morphogenetic protein-7(BMP-7), a member of the transforming growth factor superfamily, stimulates osteoblast differentiation and bone formation. There are lots of evidences supporting a direct participation of periodontal ligament(PDL) cells on periodontal tissue regeneration. The purpose of this study was to evaluate the effect of recombinant human(rh) BMP-7 on primary rat PDL cells in vitro, with special focus on the ability of bone formation. The PDL cells were cultured with rhBMP-7 at the concentration of 0, 10, 25, 50, 100 and 200ng/㎖ for MTT assay. We evaluated the alkaline phosphatase activity at 3 and 5 days of incubation and the ability to produce mineralized nodules of rat PDL cells at 14 days of cell culture in concentration of 0, 10, 25, 50 and 100ng/㎖. The cell activity was not reduced in cells treated with BMP-7 at 10～100ng/㎖, whereas the cell activity was reduced in the concentration of 200ng/㎖ than the control at day 1 and 3(p<0.01). At 3 and 5 day, alkaline phosphatase activity was significantly increased in cells treated with BMP-7 at 50ng/㎖ and 100ng/㎖(p<0.05). The area of mineralized bone nodule was greater in cells treated with BMP-7 at 50 and 100 ng/㎖ than the control(p<0.01). These results suggest that rhBMP-7 stimulate rat PDL cells to differentiate toward osteoblast phenotype and secretion of the extracellular matrix of rat PDL cells.

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관련 논문AI추천

백서 치주인대세포의 분화에 대한 Bone morphogenetic protein-7의 영향김영준, 이호재, 정현주대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제3호치주인대세포와 치은섬유아세포의 분화에 미치는 rhBMP-2의 효과에 대한 연구구영, 김경화, 김현종, 류인철, 이용무, 정종평, 최상묵, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제2호Adenovirus에 의해서 발현된 BMP-2가 치주인대세포의 분화에 미치는 영향구영, 김경화, 김태일, 류인철, 박윤정, 설양조, 이상철, 이용무, 정종평, 한수부대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제2호법랑기질 단백질 유도체가 치주인대양세포 및 조골양세포에 미치는 영향고선일, 김동운, 임성빈, 정진형대한치주과학회 · Journal of Periodontal & Implant Science2003 · 제33권 제2호치아재식시 골형태형성단백이 치주조직 치유에 미치는 영향권영혁, 김지숙, 박준봉, 이만섭, 조규성대한치주과학회 · Journal of Periodontal & Implant Science2002 · 제32권 제3호Bone Morphogenetic Protei n-4가 사람 뼈모세포와치주인대세포의 증식과 분화에 미치는 영향김지연, 방희준, 주성숙대한구강해부학회 · 대한구강해부학회지2013 · 제34권 제1호

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