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Porphyromonas gingivalis의 세균내독소가 RAW264.7세포에서의 nitric oxide의 생성과 indu- cible nitric oxide synthase의 발현에 미치는 영향 및 기전

Nitric oxide production and inducible nitric oxide synthase expression induced by Por- phyromonas gingivalis lipopolysaccharide

최점일|최은영|이주연|김성조|백은영
대한치주과학회 (The Korean Academy Of Periodontology)|2005.12|Journal of Periodontal & Implant Science|제35권 제4호|pp. 1081-1096

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참고문헌

주제분류치의학

초록

본 연구는 치주질환 주요 병인균주 중의 하나인 Porphyromonas gingivalis의 세균내독소가 마우스 대식세포주인 RAW264.7 세포에서의 nitric oxide의 생성과 iNOS의 발현에 미치는 영향을 분석하고 그 기전을 규명하기 위해 수행되었다. Butanol추출법과 phenol-water법에 의해 P. gingivalis 381로부터 세균내독소를 추출하였으며, NO의 생성은 배양 상층액 내의 nitrite 농도를 측정하여 결정하였다. 또한, iNOS의 western blot 분석과 reverse transcription (RT)-PCR 산물의 분석을 수행하였다. P. gingivalis의 세균내독소는 부가적인 자극이 없는 상태에서도 iNOS의 발현과 NO 생성을 유발하였으며, NF- κB, microtubule polymerization, protein tyrosine kinase, 그리고 protein kinase C 등이 P. gingivalis 세균내독소에 의한 NO 생성에 간여하는 것으로 여겨진다. 또한, P. gingivalis 세균내독소에 의한 NO 생성에는 L-arginine이 요구되었다. P. gingivalis 세균내독소에 의한 NO 생성은 염증성 치주질환의 발병과 진행에 있어 중요한 역할을 하는 것으로 여겨진다.

참고문헌 (38)

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관련 논문AI추천

Effects of Korean Propolis Extracts on Porphyromonas gingivalis Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 CellsKim Ki-Rim한국치위생과학회 · 치위생과학회지2025 · 제25권 제4호Porphyromonas gingivalis 자극된 RAW 264.7 cells에서 Weissella cibaria CMU의 항염 효과김민정, 김현진, 유형근대한구강해부학회 · 대한구강해부학회지2024 · 제45권 제1호Effect of quercetin on the production of nitric oxide in murine macrophages stimulated with lipopolysaccharide from Prevotella intermedia김성조, 조윤정대한치주과학회 · Journal of Periodontal & Implant Science2013 · 제43권 제4호Comparison of Cytokine Gene Induction in RAW 264.7 Cells by Porphyromonas gingivalis and Escherichia coli Lipopolysaccharide정진, 나희삼, Hae-Ryoun Park, So-Yeon Jeong, Sung-Hee Jeong, Young-Hwa Lee대한구강생물학회 · International Journal of Oral Biology2010 · 제35권 제3호Nuclear Factor-κB Dependent Induction of TNF-α and IL-1β by the Aggregatibacter actinomycetemcomitans Lipopolysaccharide in RAW 264.7 Cells정진, 김세연, 나희삼, 박미희, 정소연대한구강생물학회 · International Journal of Oral Biology2014 · 제39권 제1호고농도의 글루코스가 치주질환 병인균주의 세균내독소에 의한 염증성 cytokine 및 nitric oxide의 생성에 미치는 영향김성조대한치주과학회 · Journal of Periodontal & Implant Science2008 · 제38권 제3호

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