Gutta-percha 또는 Resilon을 사용한 근관충전이 상아세관 내의 Enterococcus faecalis에 미치는 영향
The effect of canal filling with gutta-percha or resilon on Enterococcus faecalis in bovine dentinal tubules
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목적은 근관충전이 상아세관 내에 남아있는 세균에 미치는 영향을 알아보고 가터퍼차와 resilon의 밀폐 능력을 상아세관 내에 남아있는 세균의 생존 여부를 통하여 비교하는 것이다. 발치된 우전치로 치아시편에 E. faecalis를 접종하고 제 1군은 음성대조군, 제 2군은 양성대조군, 제 3군은 ZOE 계통의 근관전색제와 가터퍼차, 제4군은 레진 계통의 근관전색제와 가터퍼차, 제 5군은 레진 계통의 근관전색제와 resilon으로 근관 충전하였다. 각 시편은 1, 2, 3주 및 4주간 보관한 뒤 round bur로 근관 내를 삭제하여 얻은 상아질을 배양하고 혼탁도를 측정하였다. 1. Nail varnish로 상아세관을 완전히 밀폐한 경우, 상아세관 내의 E. faecalis의 수는 가터퍼차 및 resilon으로 근관충전 한 경우보다 통계적으로 유의차 있게 감소하였다 (p < 0.05). 2. 레진 계통의 근관전색제와 resilon으로 근관충전 한 군에서는 시간의 흐름에 따라 상아세관 내의 E. faecalis의 수가 유의차 있게 감소하였다 (p < 0.05). 3. 가터퍼차와 resilon으로 근관충전한 군 사이에 상아세관 내에 남아있는 E. faecalis의 생존 정도는 통계학적 유의차가 없었다 (p > 0.05).
Abstract
The purpose of this study was to observe the effect of canal filling on the bacteria left in the dentinal tubules and to compare the sealing ability between Gutta-percha and Resilon. The bovine dentin block models were prepared. E. faecalis was inoculated to dentin blocks and incubated. The dentin blocks were divided into 5 groups. Group 1 was the negative control. Group 2 was the positive control. Group 3 was filled with ZOE based sealer and Gutta-percha, Group 4 with resin based sealer and Gutta-percha, and Group 5 with resin based sealer and Resilon. After 24 hour, the blocks were incubated at 37℃ for 1, 2, 3 and 4 weeks on BHI agar plates. The internal dentin portion of the blocks was removed using ISO 027, 029, 031, 035 round burs and the dentin chips were incubated at 37℃ for 24 hour. Following incubation, the optical density of the medium was measured. The data were statistically analysed using repeated measures ANOVA and one-way ANOVA. The results were as follows, 1. There was statistically significant reduction in the number of E. faecalis of the group where dentinal tubules were completely sealed with nail varnish in comparison with the groups obturated with gutta-percha or resilon (p < 0.05). 2. In group 5, the number of E. faecalis in the dentinal tubules decreased significantly with time (p < 0.05), whereas in Group 3 and 4, there was no reduction in its number (p > 0.05). 3. Under the conditions of this experiment, E. faecalis survived up to 4 weeks after obturation with gutta-percha or resilon (p > 0.05).
참고문헌 (48)
- Kakehashi S, "The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats", 20, pp.340~349 (1965)
- Dahlen G, "Influence on periapical tissues of indigenous oral bacteria and necrotic pulp tissue in monkeys", pp.475~484 (1981)
- Diss, "Bacteriological studies of nectrotic dental pulps", pp.1~7 94 (1976), Umea Univ
- m A, "Bacteriologic evaluation of the efficacy of mechanical root canal instrumentation in endodontic therapy", pp.321~328 (1981)
- Abou-Rass M, "The effectiveness of four clinical irrigation methods on the removal of root canal debris", pp.323~328
- m A, "The antibacterial effect of camphorated paramonochlorophenol camphorated phenol and calcium hydroxide in the treatment of infectedroot canals", pp.170~175 (1985)
- gren U, "The antimicrobial effect of calcium hydroxide as a short-term intracanal dressing", pp.119~125 (1991)
- Chong BS, "The role of intracanal medication in root canal treatment", pp.97~106
- Walton RE, "Principles and Practice of Endodontics", pp.232~234 (2002), Saunders company
- Peters LB, "Effects of instrumentation irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions", pp.13~21 (2002)
- Siren EK, "Inactivation of local root canal medicaments by dentine an in vitro study", pp.126~131 (2000)
- Portenier I, "Inactivation of root canal medicaments by dentine", pp.184~188 (2001)
- ？rstavik D, "Disinfection by endodontic irrigants and dressings of experimentally infected dentinal tubules", pp.142~149
- Estrela C, "Antimicrobial evaluation of calcium hydroxide in infected dentinal tubules", 25, pp.416~418 (1999)
- Molander A, "Microbiological status of root-filled teeth with apical periodontitis", 31, pp.1~7 (1998)
- Sundqvist G, "Microbiological analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment", 35, pp.86~93 (1998)
- Evans M, "Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide", 35, pp.221~228 (2002)
- Kim SK, "Influence of calcium hydroxide intracanal medication on apical seal", 35, pp.623~628 (2002)
- Soltanoff W, "A comparative study of the single-visit and the multiple-visit edodontic procedure", 4, pp.278~281 (1978)
- Oliet S, "Single-visit endodontics:a clinical study", pp.147~152
- Peters LB, "Periapical healing of endodontically treated teeth in one and two visits obturated in the presence or absence of detectable microorganisms", 35, pp.660~667 (2002)
- Moorer WR, "Evidence for antibacterial activity of endodontic gutta-percha cones", pp.503~507
- Heling I, "The antimicrobial effect within dentinal tubules of four root canal sealers", 22, pp.257~259 (1996)
- Kaplan AE, "Antimicrobial effect of six endodontic sealers an in vitro evaluation", pp.42~45 (1999)
- Fuss Z, "Effect of various mixing ratios on antibacterial properties and hardness of endodontic sealers", 26, pp.519~522 (2000)
- Siqueira Junior JF, "Antimicrobial activity and flow rate of newer and established root canal sealers", 26, pp.274~277 (2000)
- Saleh IM, "Survival of Enterococcus faecalis in infected dentinal tubules after root canal filling with different root canal sealers in vitro", 37, pp.193~198 (2004)
- Peters LB, "The fate and the role of bacteria left in root dentinal tubules", 28, pp.95~99 (1995)
- Weiger R, "Influence of calcium hydroxide intracanal dressings on the prognosis of teeth with endodontically induced periapical lesions", 33, pp.219~226 (2000)
- Swanson K, "An evaluation of coronal microleakage in endodontically treated teeth", 13, pp.56~59 (1987)
- Torabinejad M, "In vitro bacterial penetration of coronally unsealed endodontically treated teeth", 16, pp.566~569 (1990)
- Khayat A, "Human saliva penetration of coronally unsealed obturated root canals", 19, pp.458~461 (1993)
- Magura ME, "Human saliva coronal microleakage in obturated root canals an in vitro study", pp.324~331 (1991)
- Gish SP, "bacterial penetration through obturated canals following post preparation", pp.1369~1372 (1994)
- Tidmarsh BG, "Acid-cleansed and resin-sealed root canals", 4, pp.117~21 (1978)
- Leonard JE, "Apical and coronal seal of roots obturated with a dentine bonding agent and resin", 29, pp.76~83 (1996)
- Ferrari M, "Apical seal of roots obturated with laterally condensed gutta-percha", man(cci f), pp.41~4 (1998), epoxy resin cement
- Mounce R, "Another quantum leap forward for endodontics", pp.13~22 (2004)
- Shipper G, "An evaluation of microbial leakage in roots filled with a thermoplastic synthetic polymer-based root canal filling material", pp.342~347 (2004)
- Teixeira FB, "Fracture resistance of roots endodontically treated with a new resin filling material", 135, pp.646~652 (2004)
- Hata G, "Sealing ability of Thermafil with and without sealer", pp.322~326
- Scand J Dent Res 91, "Weight loss of endodontic sealers cements and pastes in water", pp.316~319 (1983)
- Peters DD, "Two-year in vitro solubility evaluation of four Gutta-percha sealer obturation techniques", 12, pp.139~145 (1986)
- Tronstad L, "Solubility and biocompatibility of calcium hydroxide-containing root canal sealers", 4, pp.152~159 (1988)
- Kontakiotis EG, "Effect of sealer thickness on long-term sealing ability:a 2-year follow-up study", 30, pp.307~312 (1997)
- Wu MK, "Sealer distribution in root canals obturated by three techniques", 33, pp.340~345 (2000)
- Nair PN, "Intraradicular bacteria and fungi in root-filled asymptomatic human teeth with therapy-resistant periapical lesions", pp.580~588 (1990)
- Nair PN, "Intraradicular bacteria and fungi in root-filled asymptomatic human teeth with therapy-resistant periapical lesions", pp.580~588 (1990)