치주인대세포에서 Aggregatibacter actinomycetemcomitans의 IL-8 및 활성산소종 유도능
Induction of IL-8 and reactive oxygen species in periodontal ligament cells by Aggregatibacter actinomycetemcomitans
1
조회
0
다운로드
0
인용
0
참고문헌
초록
Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process. (J Korean Acad Periodontol 2009;39:331-337)
Abstract
Purpose: Interleukin (IL)-8 is one of pro-inflammatory cytokines. Reactive oxygen species (ROS) are reduced metabolites of O2. Aggregatibacter actinomycetemcomitans is one of representative periodontopathogens. To investigate the role of A. actinomycetemcomitans in IL-8 expression of periodontal ligament (PDL) cells, we estimated the production of IL-8 and ROS in A. actinomycetemcomitans treated PDL cells. Methods: The IL-8 production was determined by enzyme-linked immunosorbent assay. The ROS production was estimated using H2DCFDA and FACS. Results: A. actinomycetemcomitans increased the production of IL-8 and ROS at 10, 100, and 500 multiplicity of infection. N-acetylcysteine, an antioxidant of ROS, down-regulated the production of IL-8 induced by A. actinomycetemcomitans. Conclusions: These results suggest that A. actinomycetemcomitans induces IL-8 production and ROS may act as a mediator in this process. (J Korean Acad Periodontol 2009;39:331-337)
참고문헌 (32)
- Ishikawa I, "Host responses in periodontal diseases: a preview", Periodontol 2000, 43, pp.9~13 (2007)
- Baggiolini M, "Interleukin-8, a chemotactic and inflammatory cytokine", FEBS Lett, 307, pp.97~101 (1993)
- Tanaka S, "Signaling axis in osteoclast biology and therapeutic targeting in the RANKL", RANK, Am J Nephrol, pp.2007, 27[DOI]
- Bendre MS, "Interleukin-8 stimulation of osteoclastogenesis and bone resorption is a mechanism for the increased osteolysis of metastatic bone disease", Bone, 33, pp.28~37 (2003)
- Bendre MS, "Tumor-derived interleukin-8 stimulates osteolysis independent of the receptor activator of nuclear factor-κB ligand pathway", Cancer Res, 65, pp.11001~11009 (2005)
- Tsai CC, "Levels of interleukin-1 β and interleukin-8 in gingival crevicular fluids in adult periodontitis", J Periodontol, 66, pp.852~859 (1995)
- Gamonal J, "Levels of interleukin-1 β, -8, and -10 and RANTES in gingival crevicular fluid and cell populations in adult periodontitis patients and the effect of periodontal treatment", J Periodontol, 71, pp.1535~1545 (2000)
- Gamonal J, "Characterization of cellular infiltrate, detection of chemokine receptor CCR5 and interleukin-8 and RANTES chemokines in adult periodontitis", J Periodontal Res, 36, pp.194~203 (2001)
- Socransky SS, "Periodontal microbial ecology", Periodontol 2000, 38, pp.135~187 (2005)
- Yamaji Y, "Inflammatory cytokine gene expression in human periodontal ligament fibroblasts stimulated with bacterial lipopolysaccharides", Infect Immun, 63, pp.3576~3581 (1995)
- Yamamoto T, "Cytokine production in human periodontal ligament cells stimulated with Porphyromonas gingivalis", J Periodontal Res, 41, pp.554~559 (2006)
- Lee SH, "Upregulation of intercellular adhesion molecule 1 and proinflammatory cytokines by the major surface proteins of Treponema maltophilum and Treponema lecithinolyticum, the phylogenetic group IV oral spirochetes associated with periodontitis and endodontic infections", Infect Immun, 73, pp.268~276 (2005)
- Patil C, "Actinobacillus actinomycetemcomitans lipopolysaccharide induces interleukin-6 expression through multiple mitogen-activated protein kinase pathways in periodontal ligament fibroblasts", Oral Microbiol Immunol, 21, pp.392~398 (2006)
- Droge W, "Free radicals in the physiological control of cell function", Physiol Rev, 82, pp.47~95 (2002)
- Lakshminarayanan V, "Differential regulation of interleukin-8 and intercellular adhesion molecule-1 by H2O2 and tumor necrosis factor-α in endothelial and epithelial cells", J Biol Chem, 272, pp.32910~32918 (1997)
- Verhasselt V, "Oxidative stress up-regulates IL-8 and TNF-α synthesis by human dendritic cells", Eur J Immunol, 28, pp.3886~3890 (1998)
- Josse C, "Piette J. Importance of post-transcriptional regulation of chemokine genes by oxidative stress", Biochem J, 360, pp.321~333 (2001)
- Hwang YS, "Interleukin-1α stimulates IL-8 expression through MAP kinase and ROS signaling in human gastric carcinoma cells", Oncogene, 23, pp.6603~6611 (2004)
- Pelaia G, "Effects of hydrogen peroxide on MAPK activation, IL-8 production and cell viability in primary cultures of human bronchial epithelial cells", J Cell Biochem, 93, pp.142~152 (2004)
- Yang-Sin Lee, "Induction of IL-8 in Periodontal Ligament Cells by H₂O₂", The Journal of Microbiology, 46(5), pp.579~584 (2008), 한국미생물학회
- Seo JY, "Transcriptional regulation by thiol compounds in Helicobacter pylori-induced interleukin-8 production in human gastric epithelial cells", Ann N Y Acad Sci, 973, pp.541~545 (2002)
- Christersson LA, "Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. I. Light, immunofluorescence and electron microscopic studies", J Periodontol, 58, pp.529~539 (1987)
- Christersson LA, "Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. II. Correlation between immunofluorescence and culture techniques", J Periodontol, 58, pp.540~545 (1987)
- Barnes PJ, "Nuclear factor-κB: a pivotal transcription factor in chronic inflammatory diseases", N Engl J Med, 336, pp.1066~1071 (1997)
- Meyer M, "H2O2 and antioxidants have opposite effects on activation of NF-κ B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor", Embo J, 12, pp.2005~2015 (1993)
- DeForge LE, "Regulation of interleukin 8 gene expression by oxidant stress", J Biol Chem, 268, pp.25568~25576 (1993)
- O'Hara AM, "Tumor necrosis factor (TNF)-α-induced IL-8 expression in gastric epithelial cells: role of reactive oxygen species and AP endonuclease- 1", redox factor (Ref)-1, 2009, pp.46, 359~369
- Do Young Kim, "N-Acetylcysteine Prevents LPS-Induced Pro-inflammatory Cytokines and MMP2 Production in Gingival Fibroblasts", Archives of Pharmacal Research, 30(10), pp.1283~1292 (2007), 대한약학회
- Di Paola R, "Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis", J Clin Periodontol, 32, pp.1062~1068 (2005)
- Toker H, "N-acetylcysteine, a thiol antioxidant, decreases alveolar bone loss in experimental periodontitis in rats", J Periodontol, 80, pp.672~678 (2009)
- Long P, "Low magnitude of tensile strain inhibits IL-1β-dependent induction of pro-inflammatory cytokines and induces synthesis of IL-10 in human periodontal ligament cells in vitro", J Dent Res, 80, pp.1416~1420 (2001)
- Lee HJ, "Roles of p38 and ERK MAP kinases in IL-8 expression in TNF-α- and dexamethasone- stimulated human periodontal ligament cells", Cytokine, 35, pp.67~76 (2006)
