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Identification of Enterococcus faecalis antigens specifically expressed in vivo

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Objectives: Molecular mechanism of the pathogenicity of Enterococcus faecalis (E. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here we report the identification of in vivo expressed antigens of E. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (CMAT) and an experimental animal model of endodontic infection. Materials and Methods: Among 4,500 E. coli recombinant clones screened, 19 positive clones reacted reproducibly with hyperimmune sera obtained from rabbits immunized with E. faecalis cells isolated from an experimental endodontic infection. DNA sequences from 16 of these in vivo-induced (IVI) genes were determined. Results: Identified protein antigens of E. faecalis included enzymes involved in housekeeping functions, copper resistance protein, putative outer membrane proteins, and proteins of unknown function. Conclusions: In vivo expressed antigens of E. faecalis could be identified by using a novel immune-screening technique CMAT and an experimental animal model of endodontic infection. Detailed analysis of these IVI genes will lead to a better understanding of the molecular mechanisms involved in the endodontic infection of E. faecalis.

Abstract

Objectives: Molecular mechanism of the pathogenicity of Enterococcus faecalis (E. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here we report the identification of in vivo expressed antigens of E. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (CMAT) and an experimental animal model of endodontic infection. Materials and Methods: Among 4,500 E. coli recombinant clones screened, 19 positive clones reacted reproducibly with hyperimmune sera obtained from rabbits immunized with E. faecalis cells isolated from an experimental endodontic infection. DNA sequences from 16 of these in vivo-induced (IVI) genes were determined. Results: Identified protein antigens of E. faecalis included enzymes involved in housekeeping functions, copper resistance protein, putative outer membrane proteins, and proteins of unknown function. Conclusions: In vivo expressed antigens of E. faecalis could be identified by using a novel immune-screening technique CMAT and an experimental animal model of endodontic infection. Detailed analysis of these IVI genes will lead to a better understanding of the molecular mechanisms involved in the endodontic infection of E. faecalis.

참고문헌 (38)

  1. Finlay BB, "Common themes in microbial pathogenicity revisited", Microbiol Mol Biol Rev, 61, pp.136~169 (1997)
  2. Falkow S, "Molecular Koch’s postulates applied to microbial pathogenicity", Rev Infect Dis, 10(Suppl 2), pp.S274~S276 (1988)
  3. Sundqvist G, "Prevalence of black-pigmented bacteroides species in root canal infections", J Endod, 15, pp.13~19 (1989)
  4. Griffee MB, "The relationship of Bacteroides melaninogenicus to symptoms associated with pulpal necrosis", Oral Surg Oral Med Oral Pathol, 50, pp.457~461 (1980)
  5. Sundqvist GK, "Capacity of anaerobic bacteria from necrotic dental pulps to induce purulent infections", Infect Immun, 25, pp.685~693 (1979)
  6. Sundqvist G, "Microbiologic analysis of teeth with failed endodontic treatment and the outcome of conservative retreatment", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85, pp.86~93 (1998)
  7. Hancock HH 3rd, "Bacteria isolated after unsuccessful endodontic treatment in a North American population", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91, pp.579~586 (2001)
  8. Kayaoglu G, "Virulence factors of Enterococcus faecalis: relationship to endodontic disease", Crit Rev Oral Biol Med, 15, pp.308~320 (2004)
  9. Smith H, "Questions about the behaviour of bacterial pathogens in vivo", Philos Trans R Soc Lond B Biol Sci, 355, pp.551~564 (2000)
  10. Angelichio MJ, "In vivo expression technology", Infect Immun, 70, pp.6518~6523 (2002)
  11. Mahan MJ, "Selection for bacterial genes that are specifically induced in host tissues: the hunt for virulence factors", Infect Agents Dis, 2, pp.263~268 (1993)
  12. Hensel M, "Simultaneous identification of bacterial virulence genes by negative selection", Science, 269, pp.400~403 (1995)
  13. Valdivia RH, "Fluorescence-based isolation of bacterial genes expressed within host cells", Science, 277, pp.2007~2011 (1997)
  14. Ramachandran N, "Next-generation high-density self-assembling functional protein arrays", Nat Methods, 5, pp.535~538 (2008)
  15. Rollins SM, "In vivo induced antigen technology (IVIAT)", Cell Microbiol, 7, pp.1~9 (2005)
  16. Handfield M, "In vivo induced antigen technology (IVIAT) and change mediated antigen technology (CMAT)", Infect Disord Drug Targets, 6, pp.327~334 (2006)
  17. Lee SW, "The hemagglutinin genes hagB and hagC of Porphyromonas gingivalis are transcribed in vivo as shown by use of a new expression vector", Infect Immun, 64, pp.4802~4810 (1996)
  18. Yoo JY, "Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease", FEMS Microbiol Lett, 275, pp.344~352 (2007)
  19. Handfield M, "IVIAT: a novel method to identify microbial genes expressed specifically during human infections", Trends Microbiol, 8, pp.336~339 (2000)
  20. Cheng S, "Identification of Candida albicans genes induced during thrush offers insight into pathogenesis", Mol Microbiol, 48, pp.1275~1288 (2003)
  21. Hang L, "Use of in vivo-induced antigen technology (IVIAT)to identify genes uniquely expressed during human infection with Vibrio cholerae", Proc Natl Acad Sci U S A, 100, pp.8508~8513 (2003)
  22. Deb DK, "Selective identification of new therapeutic targets of Mycobacterium tuberculosis by IVIAT approach", Tuberculosis (Edinb), 82, pp.175~182 (2002)
  23. Kim YR, "Characterization and pathogenic significance of Vibrio vulnificus antigens preferentially expressed in septicemic patients", Infect Immun, 71, pp.5461~5471 (2003)
  24. John M, "Use of in vivo-induced antigen technology for identification of Escherichia coli O157:H7 proteins expressed during human infection", Infect Immun, 73, pp.2665~2679 (2005)
  25. Harris JB, "Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi", Infect Immun, 74, pp.5161~5168 (2006)
  26. Salim KY, "Identification of group A Streptococcus antigenic determinants upregulated in vivo", Infect Immun, 73, pp.6026~6038 (2005)
  27. Cao SL, "in vivo induced antigenic determinants of Actinobacillus actinomycetemcomitans", FEMS Microbiol Lett, 237, pp.97~103 (2004)
  28. Song YH, "Genes of periodontopathogens expressed during human disease", Ann Periodontol, 7, pp.38~42 (2002)
  29. Gu H, "Use of in vivo-induced antigen technology (IVIAT) for the identification of Streptococcus suis serotype 2 in vivo-induced bacterial protein antigens", BMC Microbiol, 9, pp.201~ (2009)
  30. Lee HR, "In vivo-induced antigenic determinants of Fusobacterium nucleatum subsp. nucleatum", Mol Oral Microbiol, 26, pp.164~172 (2011)
  31. Savli H, "Expression stability of six housekeeping genes: A proposal for resistance gene quantification studies of Pseudomonas aeruginosa by real-time quantitative RT-PCR", J Med Microbiol, 52, pp.403~408 (2003)
  32. Vandecasteele SJ, "Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions", J Bacteriol, 183, pp.7094~7101 (2001)
  33. Widada J, "Quantification of the carbazole 1,9a-dioxygenase gene by real-time competitive PCR combined with coextraction of internal standards", FEMS Microbiol Lett, 202, pp.51~57 (2001)
  34. Espirito Santo C, "Contribution of copper ion resistance to survival of Escherichia coli on metallic copper surfaces", Appl Environ Microbiol, 74, pp.977~986 (2008)
  35. Grass G, "Metallic copper as an antimicrobial surface", Appl Environ Microbiol, 77, pp.1541~1547 (2011)
  36. Walters S, "A member of the peptidase M48superfamily of Porphyromonas gingivalis is associated with virulence in vitro and in vivo", J Oral Microbiol, 1 (2009)
  37. Yuan L, "The Porphyromonas gingivalis clpB gene is involved in cellular invasion in vitro and virulence in vivo", FEMS Immunol Med Microbiol, 51, pp.388~398 (2007)
  38. Yuan L, "Porphyromonas gingivalis htrA is involved in cellular invasion and in vivo survival", Microbiology, 154, pp.1161~1169 (2008)

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