Development of animal experimental periodontitis models
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Purpose: An animal periodontitis model is essential for research on the pathogenesis and treatment of periodontal disease. In this study, we have introduced a lipopolysaccharide (LPS) of a periodontal pathogen to the alveolar bone defect of experimental animals and investigated its suitability as a periodontitis model. Methods: Alveolar bone defects were made in both sides of the mandibular third premolar region of nine beagle dogs. Then,the animals were divided into the following groups: silk ligature tied on the cervical region of tooth group, Porphyromonas gingivalis LPS (P.g. LPS)-saturated collagen with silk ligature group, and no ligature or P.g. LPS application group as the control. The plaque index and gingival index were measured at 0 and 4 weeks postoperatively. The animals were then euthanized and prepared for histologic evaluation. Results: The silk ligature group and P.g. LPS with silk ligature group showed a significantly higher plaque index at 4 weeks compared to the control (P<0.05). No significant difference was found in the plaque index between the silk ligature group and P.g. LPS with silk ligature group. The P.g. LPS with silk ligature group showed a significantly higher gingival index compared to the silk ligature group or the control at 4 weeks (P<0.05). Histologic examination presented increased inflammatory cell infiltration in the gingival tissue and alveolar bone of the P.g. LPS with silk ligature group. Conclusions: An additional P.g. LPS-saturated collagen with silk ligature ensured periodontal inflammation at 4 weeks. Therefore, P.g. LPS with silk ligature application to surgically created alveolar bone defects may be a candidate model for experimental periodontitis.
Abstract
Purpose: An animal periodontitis model is essential for research on the pathogenesis and treatment of periodontal disease. In this study, we have introduced a lipopolysaccharide (LPS) of a periodontal pathogen to the alveolar bone defect of experimental animals and investigated its suitability as a periodontitis model. Methods: Alveolar bone defects were made in both sides of the mandibular third premolar region of nine beagle dogs. Then,the animals were divided into the following groups: silk ligature tied on the cervical region of tooth group, Porphyromonas gingivalis LPS (P.g. LPS)-saturated collagen with silk ligature group, and no ligature or P.g. LPS application group as the control. The plaque index and gingival index were measured at 0 and 4 weeks postoperatively. The animals were then euthanized and prepared for histologic evaluation. Results: The silk ligature group and P.g. LPS with silk ligature group showed a significantly higher plaque index at 4 weeks compared to the control (P<0.05). No significant difference was found in the plaque index between the silk ligature group and P.g. LPS with silk ligature group. The P.g. LPS with silk ligature group showed a significantly higher gingival index compared to the silk ligature group or the control at 4 weeks (P<0.05). Histologic examination presented increased inflammatory cell infiltration in the gingival tissue and alveolar bone of the P.g. LPS with silk ligature group. Conclusions: An additional P.g. LPS-saturated collagen with silk ligature ensured periodontal inflammation at 4 weeks. Therefore, P.g. LPS with silk ligature application to surgically created alveolar bone defects may be a candidate model for experimental periodontitis.
참고문헌 (30)
- Pihlstrom BL, "Periodontal diseases", Lancet, 366, pp.1809~1820 (2005)
- Schou S, "Non-human primates used in studies of periodontal disease pathogenesis : a review of the literature", J Periodontol, 64, pp.497~508 (1993)
- Yamasaki A, "Ultrastructure of the junctional epithelium of germfree rat gingiva", J Periodontol, 50, pp.641~648 (1979)
- Eggert FM, "The gingival epithelium of rodent molars with limited eruption", Acta Anat, 107, pp.297~306 (1980)
- Attström R, "Clinical and histologic characteristics of normal gingiva in dogs", J Periodontal Res, 10, pp.115~127 (1975)
- Haney JM, "Periodontal repair in dogs : evaluation of the natural disease model", J Clin Periodontol, 22, pp.208~213 (1995)
- Wikesjo UM, "Periodontal repair in dogs : supraalveolar defect models for evaluation of safety and efficacy of periodontal reconstructive therapy", J Periodontol, 65, pp.1151~1157 (1994)
- Holland M, "Radiographic bone loss correlations and technetium-99m-MDP bone uptake in ligature-induced periodontal disease in the beagle", Vet Radiol Ultrasound, 39, pp.366~374 (1998)
- Clergeau LP, "Healing response to anorganic bone implantation in periodontal intrabony defects in dogs. Part I. Bone regeneration. A microradiographic study", J Periodontol, 67, pp.140~149 (1996)
- Hayashi C, "Injectable calcium phosphate bone cement provides favorable space and a scaffold for periodontal regeneration in dogs", J Periodontol, 77, pp.940~946 (2006)
- Andrian E, "Porphyromonas gingivalis-epithelial cell interactions in periodontitis", J Dent Res, 85, pp.392~403 (2006)
- Dumitrescu AL, "A model of periodontitis in the rat: effect of lipopolysaccharide on bone resorption, osteoclast activity, and local peptidergic innervation", J Clin Periodontol, 31, pp.596~603 (2004)
- Silness J, "Periodontal disease in pregnancy: II. Correlation between oral hygiene and periodontal condtion", Acta Odontol Scand, 22, pp.121~135 (1964)
- Loe H, "Periodontal disease in pregnancy. I. Prevalence and severity", Acta Odontol Scand, 21, pp.533~551 (1963)
- Page RC, "Pathogenesis of inflammatory periodontal disease : a summary of current work", Lab Invest, 34, pp.235~249 (1976)
- Lallam-Laroye C, "Periodontitis destructions are restored by synthetic glycosaminoglycan mimetic", J Biomed Mater Res A, 79, pp.675~683 (2006)
- Peruzzo DC, "Chronic stress may modulate periodontal disease : a study in rats", J Periodontol, 79, pp.697~704 (2008)
- Lindhe J, "Experimental periodontitis in the beagle dog", J Periodontal Res, 8, pp.1~10 (1973)
- Allaker RP, "Prevalence of Porphyromonas and Prevotella species in the dental plaque of dogs", Vet Rec, 140, pp.147~148 (1997)
- Garrison SW, "LPS-elicited secretory responses in monocytes : altered release of PGE2 but not IL-1 beta in patients with adult periodontitis", J Periodontal Res, 24, pp.88~95 (1989)
- Page RC., "The role of inflammatory mediators in the pathogenesis of periodontal disease", J Periodontal Res, 26(3), pp.230~242 (1991)
- Rogers JE, "Actinobacillus actinomycetemcomitans lipopolysaccharide-mediated experimental bone loss model for aggressive periodontitis", J Periodontol, 78, pp.550~558 (2007)
- Egelberg J., "Local effect of diet on plaque formation and development of gingivitis in dogs: 3. Effect of frequency of meals and tube feeding", Odontol Revy, 16, pp.50~60 (1965)
- Hamp SE, "Experimental periodontitis in the beagle dog", J Periodontal Res, (10), pp.13~14 (1972)
- Lindhe J, "Experimental periodontitis in the beagle dog", Int Dent J, 23, pp.432~437 (1973)
- Lindhe J, "Effect of ligature placement and dental plaque on periodontal tissue breakdown in the dog", J Periodontol, 49, pp.343~350 (1978)
- Lindemann RA, "Production of interleukin-1 and tumor necrosis factor by human peripheral monocytes activated by periodontal bacteria and extracted lipopolysaccharides", J Dent Res, 67, pp.1131~1135 (1988)
- Garrison SW, "Lipopolysaccharidestimulated PGE2 release from human monocytes. Comparison of lipopolysaccharides prepared from suspected periodontal pathogens", J Periodontol, 59, pp.684~687 (1988)
- Jiang Y, "Bacteria induce osteoclastogenesis via an osteoblast-independent pathway", Infect Immun, 70, pp.3143~3148 (2002)
- Page RC, "Spontaneous chronic periodontitis in adult dogs : a clinical and histopathological survey", J Periodontol, 52, pp.60~73 (1981)