Physicochemical characterization of porcine bone-derived grafting material and comparison with bovine xenografts for dental applications
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Purpose: The physicochemical properties of a xenograft are very important because they strongly influence the bone regeneration capabilities of the graft material. Even though porcine xenografts have many advantages, only a few porcine xenografts are commercially available, and most of their physicochemical characteristics have yet to be reported. Thus, in this work we aimed to investigate the physicochemical characteristics of a porcine bone grafting material and compare them with those of 2 commercially available bovine xenografts to assess the potential of xenogenic porcine bone graft materials for dental applications. Methods: We used various characterization techniques, such as scanning electron microscopy, the Brunauer-Emmett-Teller adsorption method, atomic force microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and others, to compare the physicochemical properties of xenografts of different origins. Results: The porcine bone grafting material had relatively high porosity (78.4%) and a large average specific surface area (SSA; 69.9 m2/g), with high surface roughness (10-point average roughness, 4.47 μm) and sub-100-nm hydroxyapatite crystals on the surface. Moreover, this material presented a significant fraction of sub-100-nm pores, with negligible amounts of residual organic substances. Apart from some minor differences, the overall characteristics of the porcine bone grafting material were very similar to those of one of the bovine bone grafting material. However, many of these morphostructural properties were significantly different from the other bovine bone grafting material, which exhibited relatively smooth surface morphology with a porosity of 62.0% and an average SSA of 0.5 m2/g. Conclusions: Considering that both bovine bone grafting materials have been successfully used in oral surgery applications in the last few decades, this work shows that the porcine-derived grafting material possesses most of the key physiochemical characteristics required for its application as a highly efficient xenograft material for bone replacement.
Abstract
Purpose: The physicochemical properties of a xenograft are very important because they strongly influence the bone regeneration capabilities of the graft material. Even though porcine xenografts have many advantages, only a few porcine xenografts are commercially available, and most of their physicochemical characteristics have yet to be reported. Thus, in this work we aimed to investigate the physicochemical characteristics of a porcine bone grafting material and compare them with those of 2 commercially available bovine xenografts to assess the potential of xenogenic porcine bone graft materials for dental applications. Methods: We used various characterization techniques, such as scanning electron microscopy, the Brunauer-Emmett-Teller adsorption method, atomic force microscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, and others, to compare the physicochemical properties of xenografts of different origins. Results: The porcine bone grafting material had relatively high porosity (78.4%) and a large average specific surface area (SSA; 69.9 m2/g), with high surface roughness (10-point average roughness, 4.47 μm) and sub-100-nm hydroxyapatite crystals on the surface. Moreover, this material presented a significant fraction of sub-100-nm pores, with negligible amounts of residual organic substances. Apart from some minor differences, the overall characteristics of the porcine bone grafting material were very similar to those of one of the bovine bone grafting material. However, many of these morphostructural properties were significantly different from the other bovine bone grafting material, which exhibited relatively smooth surface morphology with a porosity of 62.0% and an average SSA of 0.5 m2/g. Conclusions: Considering that both bovine bone grafting materials have been successfully used in oral surgery applications in the last few decades, this work shows that the porcine-derived grafting material possesses most of the key physiochemical characteristics required for its application as a highly efficient xenograft material for bone replacement.
참고문헌 (33)
- Park SY, "Aspartic acid-assisted synthesis of multifunctional strontium-substituted hydroxyapatite microspheres", Cryst Growth Des, 16, pp.4318~4326 (2016)
- Kim DH, "Dental hetero-graft materials with nano hydroxyapatite surface treatment", J Nanosci Nanotechnol, 15, pp.7942~7949 (2015)
- Richardson CR, "Clinical evaluation of Bio-Oss® : a bovine-derived xenograft for the treatment of periodontal osseous defects in humans", J Clin Periodontol, 26, pp.421~428 (1999)
- Tadic D, "A thorough physicochemical characterisation of 14 calcium phosphate-based bone substitution materials in comparison to natural bone", Biomaterials, 25, pp.987~994 (2004)
- Hölzer A, "The interrelation of trabecular microstructural parameters of the greater tubercle measured for different species", J Orthop Res, 30, pp.429~434 (2012)
- Lorenzen E, "A review of the human vs. porcine female genital tract and associated immune system in the perspective of using minipigs as a model of human genital Chlamydia infection", Vet Res, 46, pp.116~ (2015)
- Salamanca E, "A novel porcine graft for regeneration of bone defects", Materials(Basel), 8, pp.2523~2536 (2015)
- ) : a radiological and histomorphometric study, "Clin Oral Implants Res", 2011, 767~773
- Go A, "Osteogenic effect of low-temperature-heated porcine bone particles in a rat calvarial defect model", J Biomed Mater Res A, 102, pp.3609~3617 (2014)
- Mochalov KE, "Combined scanning probe nanotomography and optical microspectroscopy : a correlative technique for 3D characterization of nanomaterials", ACS Nano, 7, pp.8953~8962 (2013)
- Mochalov KE, "High-resolution scanning near-field optical nanotomography : a technique for 3D multimodal nanoscale characterization of nano-biophotonic materials", Phys Procedia, 73, pp.168~172 (2015)
- Mariotti F, "Converting nitrogen into protein--beyond 6.25 and Jones' factors", Crit Rev Food Sci Nutr, 48, pp.177~184 (2008)
- Renders GA, "Porosity of human mandibular condylar bone", J Anat, 210, pp.239~248 (2007)
- Hing KA, "Microporosity enhances bioactivity of synthetic bone graft substitutes", J Mater Sci Mater Med, 16, pp.467~475 (2005)
- Lee DS, "Physicochemical characterization of InterOss® and Bio-Oss® anorganic bovine bone grafting material for oral surgery : a comparative study", Mater Chem Phys, 146, pp.99~104 (2014)
- Lowell S, "Powder surface area and porosity" (1991), Springers
- Webb PA, "Analytical methods in fine particle technology" (1997), Micromeritics Instrument Corporation
- Lee H, "Surface potential analysis of nanoscale biomaterials and devices using kelvin probe force microscopy", J Nanomater, 2016, pp.4209130~ (2016)
- Figueiredo M, "Physicochemical characterization of biomaterials commonly used in dentistry as bone substitutes--comparison with human bone", J Biomed Mater Res B Appl Biomater, 92, pp.409~419 (2010)
- LeGeros RZ, "Calcium phosphates in oral biology and medicine", Monogr Oral Sci, 15, pp.1~201 (1991)
- Berzina-Cimdina L, "Research of calcium phosphates using Fourier transform infrared spectroscopy" (2012), INTECH Open Access Publisher
- Rivera-Muñoz EM., "Biomedical engineering: frontiers and challenges" (2011), INTECH Open Access Publisher
- Figueiredo MM, "Infrared spectroscopy: life and biomedical sciences" (2012), INTECH Open Access Publisher
- Sáez-Plaza P, "An overview of the Kjeldahl method of nitrogen determination. Part II. Sample preparation, working scale, instrumental finish, and quality control", Crit Rev Anal Chem, 43, pp.224~272 (2013)
- Cohen SA, "Amino acid analysis utilizing phenylisothiocyanate derivatives", Anal Biochem, 174, pp.1~16 (1988)
- Crabb JW, "Amino acid analysis", Curr Protoc Protein Sci (2001)
- Cowin SC, "Bone mechanics" (1989), CRC Press
- da Cruz AC, "Physico-chemical characterization and biocompatibility evaluation of hydroxyapatites", J Oral Sci, 48, pp.219~226 (2006)
- Murugan R, "Nanoporous hydroxy-carbonate apatite scaffold made of natural bone", Mater Lett, 60, pp.2844~2847 (2006)
- Webster TJ, "Enhanced functions of osteoblasts on nanophase ceramics", Biomaterials, 21, pp.1803~1810 (2000)
- Li X, "The effect of calcium phosphate microstructure on bone-related cells in vitro", Biomaterials, 29, pp.3306~3316 (2008)
- Kubies D, "The interaction of osteoblasts with bone-implant materials: 1. The effect of physicochemical surface properties of implant materials", Physiol Res, 60, pp.95~111 (2011)
- Rupp F, "A review on the wettability of dental implant surfaces I : theoretical and experimental aspects", Acta Biomater, 10, pp.2894~2906 (2014)