Phelligridin D maintains the function of periodontal ligament cells through autophagy in glucose-induced oxidative stress
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Purpose: The objective of this study was to investigate whether phelligridin D could reduce glucose-induced oxidative stress, attenuate the resulting inflammatory response, and restore the function of human periodontal ligament cells (HPDLCs). Methods: Primary HPDLCs were isolated from healthy human teeth and cultured. To investigate the effect of phelligridin D on glucose-induced oxidative stress, HPDLCs were treated with phelligridin D, various concentrations of glucose, and glucose oxidase. Glucose-induced oxidative stress, inflammatory molecules, osteoblast differentiation, and mineralization of the HPDLCs were measured by hydrogen peroxide (H2O2) generation, cellular viability, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot analyses. Results: Glucose-induced oxidative stress led to increased production of H2O2, with negative impacts on cellular viability, ALP activity, and calcium deposition in HPDLCs. Furthermore, HPDLCs under glucose-induced oxidative stress showed induction of inflammatory molecules (intercellular adhesion molecule-1, vascular cell adhesion protein-1, tumor necrosis factor-alpha, interleukin-1-beta) and disturbances of osteogenic differentiation (bone morphogenetic protein-2, and -7, runt-related transcription factor-2), cementogenesis (cementum protein-1), and autophagy-related molecules (autophagy related 5, light chain 3 I/II, beclin-1). Phelligridin D restored all these molecules and maintained the function of HPDLCs even under glucose-induced oxidative stress. Conclusions: This study suggests that phelligridin D reduces the inflammation that results from glucose-induced oxidative stress and restores the function of HPDLCs (e.g., osteoblast differentiation) by upregulating autophagy.
Abstract
Purpose: The objective of this study was to investigate whether phelligridin D could reduce glucose-induced oxidative stress, attenuate the resulting inflammatory response, and restore the function of human periodontal ligament cells (HPDLCs). Methods: Primary HPDLCs were isolated from healthy human teeth and cultured. To investigate the effect of phelligridin D on glucose-induced oxidative stress, HPDLCs were treated with phelligridin D, various concentrations of glucose, and glucose oxidase. Glucose-induced oxidative stress, inflammatory molecules, osteoblast differentiation, and mineralization of the HPDLCs were measured by hydrogen peroxide (H2O2) generation, cellular viability, alkaline phosphatase (ALP) activity, alizarin red staining, and western blot analyses. Results: Glucose-induced oxidative stress led to increased production of H2O2, with negative impacts on cellular viability, ALP activity, and calcium deposition in HPDLCs. Furthermore, HPDLCs under glucose-induced oxidative stress showed induction of inflammatory molecules (intercellular adhesion molecule-1, vascular cell adhesion protein-1, tumor necrosis factor-alpha, interleukin-1-beta) and disturbances of osteogenic differentiation (bone morphogenetic protein-2, and -7, runt-related transcription factor-2), cementogenesis (cementum protein-1), and autophagy-related molecules (autophagy related 5, light chain 3 I/II, beclin-1). Phelligridin D restored all these molecules and maintained the function of HPDLCs even under glucose-induced oxidative stress. Conclusions: This study suggests that phelligridin D reduces the inflammation that results from glucose-induced oxidative stress and restores the function of HPDLCs (e.g., osteoblast differentiation) by upregulating autophagy.
참고문헌 (40)
- Bartold PM, "Tissue engineering : a new paradigm for periodontal regeneration based on molecular and cell biology", Periodontol 2000, 24, pp.253~269 (2000)
- Hou J, "Role of ferritin in the cytodifferentiation of periodontal ligament cells", Biochem Biophys Res Commun, 426, pp.643~648 (2012)
- Hoz L, "Cementum protein 1(CEMP1)induces differentiation by human periodontal ligament cells under three-dimensional culture conditions", Cell Biol Int, 36, pp.129~136 (2012)
- 최희동, "Analysis of gene expression during mineralization of cultured human periodontal ligament cells", Journal of Periodontal & Implant Science, 41(1), pp.30~43 (2011), 대한치주과학회
- Dahiya P, "Reactive oxygen species in periodontitis", J Indian Soc Periodontol, 17, pp.411~416 (2013)
- Polak D, "An update on the evidence for pathogenic mechanisms that may link periodontitis and diabetes", J Clin Periodontol, 45, pp.150~166 (2018)
- Zheng J, "Diabetes activates periodontal ligament fibroblasts via NF-κB in vivo", J Dent Res, 97, pp.580~588 (2018)
- Preshaw PM, "Periodontitis and diabetes", Br Dent J, 227, pp.577~584 (2019)
- Lee YH, "Nanoparticle mediated PPARγ gene delivery on dental implants improves osseointegration via mitochondrial biogenesis in diabetes mellitus rat model", Nanomedicine(Lond), 13, pp.1821~1832 (2017)
- Covani U, "Relationship between human periodontitis and type 2 diabetes at a genomic level : a data-mining study", J Periodontol, 80, pp.1265~1273 (2009)
- Jansson H, "Type 2 diabetes and risk for periodontal disease : a role for dental health awareness", J Clin Periodontol, 33, pp.408~414 (2006)
- Mealey BL, "Periodontal disease and diabetes. A two-way street", J Am Dent Assoc, 137(Suppl), pp.26S~31S (2006)
- Hale AN, "Autophagy : regulation and role in development", Autophagy, 9, pp.951~972 (2013)
- White E, "Role of autophagy in suppression of inflammation and cancer", Curr Opin Cell Biol, 22, pp.212~217 (2010)
- Mizushima N, "Autophagy fights disease through cellular self-digestion", Nature, 451, pp.1069~1075 (2008)
- Wei W, "Activation of autophagy in periodontal ligament mesenchymal stem cells promotes angiogenesis in periodontitis", J Periodontol, 89, pp.718~727 (2018)
- Kamruzzaman SM, "Antiplatelet activity of Phellinus baummii methanol extract is mediated by cyclic AMP elevation and inhibition of collagen-activated integrin-α(IIb)β3 and MAP kinase", Phytother Res, 25, pp.1596~1603 (2011)
- Hwang BS, "Anti-influenza activities of polyphenols from the medicinal mushroom Phellinus baumii", Bioorg Med Chem Lett, 25, pp.3256~3260 (2015)
- Jin QL, "Antioxidant and DNA damage protecting potentials of polysaccharide extracted from Phellinus baumii using a delignification method", Carbohydr Polym, 152, pp.575~582 (2016)
- Lee S, "Anti-inflammatory phenolic metabolites from the edible fungus Phellinus baumii in LPS-stimulated RAW264.7 cells", Molecules, 22, pp.1583~ (2017)
- Kim JE, "Anti-inflammatory character of phelligridin D modulates periodontal regeneration in lipopolysaccharide-induced human periodontal ligament cells", J Periodontal Res, 53, pp.816~824 (2018)
- Kim JE, "Phelligridin D-loaded oral nanotube titanium implant enhances osseointegration and prevents osteolysis in rat mandible", Artif Cells Nanomed Biotechnol, 46, pp.397~407 (2018)
- Cho EJ, "Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob", ob mice, 2007, pp.75, 1257~1265
- Lee IK, "Styrylpyrones from the medicinal fungus Phellinus baumii and their antioxidant properties", Bioorg Med Chem Lett, 20, pp.5459~5461 (2010)
- Lee YH, "Davallialactone reduces inflammation and repairs dentinogenesis on glucose oxidase-induced stress in dental pulp cells", J Endod, 39, pp.1401~1406 (2013)
- Yu MK, "Anti-inflammatory effect of peroxisome proliferator activated receptor gamma on human dental pulp cells", J Endod, 35, pp.524~528 (2009)
- Manfredi M, "Update on diabetes mellitus and related oral diseases", Oral Dis, 10, pp.187~200 (2004)
- Giugliano D, "Oxidative stress and diabetic vascular complications", Diabetes Care, 19, pp.257~267 (1996)
- Baynes JW, "Role of oxidative stress in development of complications in diabetes", Diabetes, 40, pp.405~412 (1991)
- Halliwell B, "Hydrogen peroxide in the human body", FEBS Lett, 486, pp.10~13 (2000)
- Nishimura F, "Glucose-mediated alteration of cellular function in human periodontal ligament cells", J Dent Res, 75, pp.1664~1671 (1996)
- Kook SH, "Activation of canonical Wnt", β-catenin signaling inhibits H2O2-induced decreases in proliferation and differentiation of human periodontal ligament fibroblasts, 2016, pp.411, 83~94
- Lee DH, "Effects of hydrogen peroxide(H2O2)on alkaline phosphatase activity and matrix mineralization of odontoblast and osteoblast cell lines", Cell Biol Toxicol, 22, pp.39~46 (2006)
- Kuang Y, "Metformin prevents against oxidative stress-induced senescence in human periodontal ligament cells", Biogerontology, 21, pp.13~27 (2020)
- Dene BA, "Effects of antioxidant treatment on normal and diabetic rat retinal enzyme activities", J Ocul Pharmacol Ther, 21, pp.28~35 (2005)
- Wang WH, "Hypoglycemic effect of ethanol and ethyl acetate extract of Phellinus baumii fruiting body in streptozotocin-induced diabetic mice", Evid Based Complement Alternat Med, 2015, pp.783460~ (2015)
- Minamikawa H, "Effect of N-acetylcysteine on rat dental pulp cells cultured on mineral trioxide aggregate", J Endod, 37, pp.637~641 (2011)
- Lee YH, "The survival role of peroxisome proliferator-activated receptor gamma induces odontoblast differentiation against oxidative stress in human dental pulp cells", J Endod, 39, pp.236~241 (2013)
- Boya P, "Emerging regulation and functions of autophagy", Nat Cell Biol, 15, pp.713~720 (2013)
- An Y, "Increased autophagy is required to protect periodontal ligament stem cells from apoptosis in inflammatory microenvironment", J Clin Periodontol, 43, pp.618~625 (2016)
