본문 바로가기
KERD
논문KCI 등재

Subgingival pathogens in chronic periodontitis patients affected by type 2 diabetes mellitus: a retrospective case-control study

0

조회

0

다운로드

2

인용

0

참고문헌

키워드

주제분류치의학

초록

Purpose: The aim of this study was to compare the prevalence and bacterial load of 6 main periodontal pathogens between pairs of periodontal patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated. Methods: Twenty patients affected by chronic periodontitis and type 2 diabetes were retrospectively selected and matched to 20 patients without diabetes on the basis of the degree and severity of periodontal disease. Microbiological data of subgingival biofilms were analysed and compared for the examined pathogens: A. actinomycetemcomitans, P. gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum, and Tannerella forsythia. Results: The pairs were balanced in terms of demographic and clinical parameters, except for bleeding on probing and suppuration. In the microbiological test sites (4 for each patient), the mean probing pocket depth was 6.34±1.63 mm in patients with diabetes and 6.41±1.78 mm in patients without diabetes. No significant difference between pairs in the prevalence of P. gingivalis or the distribution of its genotypes was recorded. Patients with diabetes had a significantly greater amount of total bacterial load, P. gingivalis, T. denticola, T. forsythia, and F. nucleatum (P<0.05). Moreover, patients with diabetes had a higher number of sites with a greater cell count than patients without diabetes. When compared to the total bacterial load, only T. forsythia maintained its relative load in patients with diabetes (P=0.001). Conclusions: This retrospective matched study supports the hypothesis that microbiological differences exist among periodontal patients with and without diabetes mellitus. Trial Registration: ClinicalTrials.gov Identifier: NCT03786133

Abstract

Purpose: The aim of this study was to compare the prevalence and bacterial load of 6 main periodontal pathogens between pairs of periodontal patients with and without type 2 diabetes mellitus. Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans genotypes were also investigated. Methods: Twenty patients affected by chronic periodontitis and type 2 diabetes were retrospectively selected and matched to 20 patients without diabetes on the basis of the degree and severity of periodontal disease. Microbiological data of subgingival biofilms were analysed and compared for the examined pathogens: A. actinomycetemcomitans, P. gingivalis, Prevotella intermedia, Treponema denticola, Fusobacterium nucleatum, and Tannerella forsythia. Results: The pairs were balanced in terms of demographic and clinical parameters, except for bleeding on probing and suppuration. In the microbiological test sites (4 for each patient), the mean probing pocket depth was 6.34±1.63 mm in patients with diabetes and 6.41±1.78 mm in patients without diabetes. No significant difference between pairs in the prevalence of P. gingivalis or the distribution of its genotypes was recorded. Patients with diabetes had a significantly greater amount of total bacterial load, P. gingivalis, T. denticola, T. forsythia, and F. nucleatum (P<0.05). Moreover, patients with diabetes had a higher number of sites with a greater cell count than patients without diabetes. When compared to the total bacterial load, only T. forsythia maintained its relative load in patients with diabetes (P=0.001). Conclusions: This retrospective matched study supports the hypothesis that microbiological differences exist among periodontal patients with and without diabetes mellitus. Trial Registration: ClinicalTrials.gov Identifier: NCT03786133

참고문헌 (40)

  1. Fox CH, "New considerations in the prevalence of periodontal disease", Curr Opin Dent, 2, pp.5~11 (1992)
  2. International Diabetes Federation, "IDF Diabetes Atlas", International Diabetes Federation, ~ (2017)
  3. Kassebaum NJ, "Global burden of severe periodontitis in 1990–2010 : a systematic review and meta-regression", J Dent Res, 93, pp.1045~1053 (2014)
  4. Taylor GW, "Bidirectional interrelationships between diabetes and periodontal diseases : an epidemiologic perspective", Ann Periodontol, 6, pp.99~112 (2001)
  5. Hallmon WW, "Implications of diabetes mellitus and periodontal disease", Diabetes Educ, 18, pp.310~315 (1992)
  6. Engebretson SP, "The influence of diabetes on gingival crevicular fluid beta-glucuronidase and interleukin-8", J Clin Periodontol, 33, pp.784~790 (2006)
  7. Ficara AJ, "A comparison of the glucose and protein content of gingival fluid from diabetics and nondiabetics", J Periodontal Res, 10, pp.171~175 (1975)
  8. Pihlstrom BL, "Periodontal diseases", Lancet, 366, pp.1809~1820 (2005)
  9. Socransky SS, "Microbial complexes in subgingival plaque", J Clin Periodontol, 25, pp.134~144 (1998)
  10. Asai Y, "Genetic variation of a fimbrial protein from Porphyromonas gingivalis and its distribution in patients with periodontal diseases", Microbiol Res, 160, pp.257~263 (2005)
  11. Höglund Åberg C, "Progression of attachment loss is strongly associated with presence of the JP2 genotype of Aggregatibacter actinomycetemcomitans : a prospective cohort study of a young adolescent population", J Clin Periodontol, 41, pp.232~241 (2014)[DOI]
  12. Preshaw PM, "Periodontitis and diabetes : a two-way relationship", Diabetologia, 55, pp.21~31 (2012)
  13. Taylor JJ, "A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes", J Clin Periodontol, 40(Suppl 14), pp.S113~134 (2013)
  14. Polak D, "An update on the evidence for pathogenic mechanisms that may link periodontitis and diabetes", J Clin Periodontol, 45, pp.150~166 (2018)
  15. Lalla E, "Periodontal infection profiles in type 1 diabetes", J Clin Periodontol, 33, pp.855~862 (2006)
  16. Armitage GC, "Development of a classification system for periodontal diseases and conditions", Ann Periodontol, 4, pp.1~6 (1999)
  17. "American Academy of Periodontology task force report on the update to the 1999 Classification of Periodontal Diseases and Conditions", J Periodontol, 86, pp.835~838 (2015)
  18. Yuan K, "Detection of putative periodontal pathogens in non-insulin-dependent diabetes mellitus and non-diabetes mellitus by polymerase chain reaction", J Periodontal Res, 36, pp.18~24 (2001)
  19. Khader YS, "Periodontal status of diabetics compared with nondiabetics : a meta-analysis", J Diabetes Complications, 20, pp.59~68 (2006)
  20. Ebersole JL, "Microbiologic and immunologic characteristics of periodontal disease in Hispanic Americans with type 2 diabetes", J Periodontol, 79, pp.637~646 (2008)
  21. Casarin RC, "Subgingival biodiversity in subjects with uncontrolled type-2 diabetes and chronic periodontitis", J Periodontal Res, 48, pp.30~36 (2013)
  22. Zhou M, "Investigation of the effect of type 2 diabetes mellitus on subgingival plaque microbiota by high-throughput 16S rDNA pyrosequencing", PLoS One, 8, pp.e61516~ (2013)
  23. Field CA, "Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes", J Periodontal Res, 47, pp.470~478 (2012)
  24. Liu LS, "Differences in the subgingival microbial population of chronic periodontitis in subjects with and without type 2 diabetes mellitus-a systematic review", Clin Oral Investig, 22, pp.2743~2762 (2018)
  25. Tonetti MS, "Staging and grading of periodontitis : framework and proposal of a new classification and case definition", J Clin Periodontol, 45(Suppl 20), pp.S149~161 (2018)
  26. Joaquim CR, "The combined and individual impact of diabetes and smoking on key subgingival periodontal pathogens in patients with chronic periodontitis", J Periodontal Res, 53, pp.315~323 (2018)
  27. Gatto MR, "Prevalence of six periodontal pathogens in subgingival samples of Italian patients with chronic periodontitis", New Microbiol, 37, pp.517~524 (2014)
  28. Miranda TS, "Influence of glycemic control on the levels of subgingival periodontal pathogens in patients with generalized chronic periodontitis and type 2 diabetes", J Appl Oral Sci, 25, pp.82~89 (2017)
  29. Mealey BL, "Periodontal disease and diabetes. A two-way street", J Am Dent Assoc, 137(Suppl), pp.26S~31S (2006)
  30. Longo PL, "Glycaemic status affects the subgingival microbiome of diabetic patients", J Clin Periodontol, 45, pp.932~940 (2018)
  31. Li C, "The sociodemographic characteristics, periodontal health status, and subgingival microbiota of patients with chronic periodontitis and type 2 diabetes mellitus: a case-control study in a Chinese population", J Periodontol, 84, pp.1058~1066 (2013)
  32. Sardi JC, "Periodontal conditions and prevalence of putative periodontopathogens and Candida spp. in insulin-dependent type 2 diabetic and non-diabetic patients with chronic periodontitis--a pilot study", Arch Oral Biol, 56, pp.1098~1105 (2011)
  33. Montevecchi M, "Microbiological distribution of six periodontal pathogens between untreated Italian and Dutch periodontal patients", Oral Health Prev Dent, 14, pp.329~337 (2016)
  34. Nibali L, "Periodontal infectogenomics : systematic review of associations between host genetic variants and subgingival microbial detection", J Clin Periodontol, 43, pp.889~900 (2016)
  35. Ruscitto A, "Identification of a novel N-acetylmuramic acid transporter in Tannerella forsythia", J Bacteriol, 198, pp.3119~3125 (2016)
  36. Wyss C, "Dependence of proliferation of Bacteroides forsythus on exogenous N-acetylmuramic acid", Infect Immun, 57, pp.1757~1759 (1989)
  37. Maiden MF, "Glucose toxicity effect and accumulation of methylglyoxal by the periodontal anaerobe Bacteroides forsythus", Anaerobe, 10, pp.27~32 (2004)
  38. Herrera D, "The periodontal abscess (I). Clinical and microbiological findings", J Clin Periodontol, 27, pp.387~394 (2000)
  39. Davila-Perez C, "Distribution of genotypes of Porphyromonas gingivalis in type 2 diabetic patients with periodontitis in Mexico", J Clin Periodontol, 34, pp.25~30 (2007)
  40. Makiura N, "Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment", Oral Microbiol Immunol, 23, pp.348~351 (2008)

관련 논문AI추천

Subgingival microbiome in periodontitis and type 2 diabetes mellitus: an exploratory study using metagenomic sequencingJia Liu, Jiani Zhou, Lihong Guo, Tingjun Liu, Xianjun Lu, Zijian Yuan대한치주과학회 · Journal of Periodontal & Implant Science2022 · 제52권 제4호Efficacy of nonsurgical periodontal therapy on glycaemic control in type II diabetic patients: a randomized controlled clinical trialAmit Tirth, Pradeep Shankar Tangade, Ravishankar Lingesha Telgi, Sumit Kumar, Vaibhav Tandon, Vipul Yadav대한치주과학회 · Journal of Periodontal & Implant Science2013 · 제43권 제4호HbA1c changes in patients with diabetes following periodontal therapy김수환, 김영성, 김원경, 이영규, 이지혜대한치주과학회 · Journal of Periodontal & Implant Science2021 · 제51권 제2호Effects of non-surgical periodontal treatment with probiotic supplementation on metabolic dysfunction in patients with periodontitis: a retrospective studyChoi Eun-Mi, Cho Mu-Yeol, Eom Je-Hyun, Hwang In-Seong, Hwang Ji-Young, Hwang Ju-Young, Kim Hye-Sung, Kim Yeon-Jin, Lee Jeong-Hoo한국치위생학회 · 한국치위생학회지2025 · 제25권 제2호Efficacy of non-surgical treatment accompanied by professional toothbrushing in the treatment of chronic periodontitis in patients with type 2 diabetes mellitus: a randomized controlled clinical trial이재영, 진보형, 최영님, 최윤영대한치주과학회 · Journal of Periodontal & Implant Science2020 · 제50권 제2호Effect of scaling and root planing combined with systemic doxycycline therapy on glycemic control in diabetes mellitus subjects with chronic generalized periodontitis: a clinical studyAbhijeet R. Shete, Abhijit N. Gurav, Hitesh M. Desarda, Subodh P. Gaikwad대한치주과학회 · Journal of Periodontal & Implant Science2013 · 제43권 제2호

이 간행물의 다른 논문 (6건)