The activin/BMP-2 chimera AB204 promotes periodontal tissue regeneration in a buccal dehiscence model: a pilot study
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Purpose: A combination of activin and bone morphogenetic protein-2 (BMP-2), termed AB204, has been shown to improve osteogenic potential with fewer side effects than BMP-2 alone. This study was performed to evaluate the effect of AB204 on periodontal tissue regeneration in a dog buccal dehiscence model. Methods: Buccal dehiscence defects were created on the maxillary premolars (P1, P2, and P3) of 6 mongrel dogs. After 5 weeks, the dogs were randomly assigned to 1 of 3 groups: the control, collagen matrix (CM), and CM/AB204 groups. Grafting procedures were then performed. The dogs were sacrificed 8 weeks after the grafting procedure, and volumetric and histological analyses were conducted. Results: The thickness of the buccal gingiva in the CM/AB204 group was greater than those in the other groups at 2 weeks (P<0.05). The ridge width in the AB204/CM group exceeded the width in the other groups at 4 and 8 weeks; however, the difference was not statistically significant. Histological analysis revealed that the CM/AB204 group demonstrated the formation of new bone surrounded by newly formed periodontal ligament and cementum (P=0.035). Conclusions: The combined application of CM and AB204 shows promise in facilitating the regeneration of periodontal attachment, including the formation of new bone, cementum, and periodontal ligament.
Abstract
Purpose: A combination of activin and bone morphogenetic protein-2 (BMP-2), termed AB204, has been shown to improve osteogenic potential with fewer side effects than BMP-2 alone. This study was performed to evaluate the effect of AB204 on periodontal tissue regeneration in a dog buccal dehiscence model. Methods: Buccal dehiscence defects were created on the maxillary premolars (P1, P2, and P3) of 6 mongrel dogs. After 5 weeks, the dogs were randomly assigned to 1 of 3 groups: the control, collagen matrix (CM), and CM/AB204 groups. Grafting procedures were then performed. The dogs were sacrificed 8 weeks after the grafting procedure, and volumetric and histological analyses were conducted. Results: The thickness of the buccal gingiva in the CM/AB204 group was greater than those in the other groups at 2 weeks (P<0.05). The ridge width in the AB204/CM group exceeded the width in the other groups at 4 and 8 weeks; however, the difference was not statistically significant. Histological analysis revealed that the CM/AB204 group demonstrated the formation of new bone surrounded by newly formed periodontal ligament and cementum (P=0.035). Conclusions: The combined application of CM and AB204 shows promise in facilitating the regeneration of periodontal attachment, including the formation of new bone, cementum, and periodontal ligament.
참고문헌 (39)
- Wei L, "Periodontal regeneration using bone morphogenetic protein 2 incorporated biomimetic calcium phosphate in conjunction with barrier membrane : a pre-clinical study in dogs", J Clin Periodontol, 46, pp.1254~1263 (2019)
- James AW, "A review of the clinical side effects of bone morphogenetic protein-2", Tissue Eng Part B Rev, 22, pp.284~297 (2016)
- Miyaji H, "Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein-2 in the connective tissue of the palate", J Periodontal Res, 37, pp.204~209 (2002)
- Miyaji H, "Dentin resorption and cementum-like tissue formation by bone morphogenetic protein application", J Periodontal Res, 41, pp.311~315 (2006)
- Miyaji H, "Root surface conditioning with bone morphogenetic protein-2 facilitates cementum-like tissue deposition in beagle dogs", J Periodontal Res, 45, pp.658~663 (2010)
- Zaman KU, "Effect of recombinant human platelet-derived growth factor-BB and bone morphogenetic protein-2 application to demineralized dentin on early periodontal ligament cell response", J Periodontal Res, 34, pp.244~250 (1999)
- Yoon BH, "An activin A", BMP2 chimera, AB204, displays bone-healing properties superior to those of BMP2, 2014, pp.29, 1950~1959
- Ryu D, "Activin A", BMP2 Chimera(AB204)exhibits better spinal bone fusion properties than rhBMP2, 2018, pp.61, 669~679
- Son S, "Activin A and BMP chimera(AB204)induced bone fusion in osteoporotic spine using an ovariectomized rat model", Spine J, 20, pp.809~820 (2020)
- Zheng GB, "In vitro and in vivo evaluation of osteoinductivity and bone fusion ability of an Activin A", BMP2 Chimera(AB204) : a comparison study between AB204 and rhBMP-2, 2017, pp.35, 249~258
- Polo CI, "Synergism between recombinant human bone morphogenetic protein 2", absorbable collagen sponge and bone substitutes favors vertical bone augmentation and the resorption rate of the biomaterials : histomorphometric and 3D microcomputed tomography analysis, 2020, pp.91, 1295~1306
- Choi SH, "Effect of recombinant human bone morphogenetic protein-2", absorbable collagen sponge(rhBMP-2, J Periodontol, pp.2002, 73[DOI]
- Xiao Y, "Tissue engineering for bone regeneration using differentiated alveolar bone cells in collagen scaffolds", Tissue Eng, 9, pp.1167~1177 (2003)
- Bauer-Kreisel P, "Cell-delivery therapeutics for adipose tissue regeneration", Adv Drug Deliv Rev, 62, pp.798~813 (2010)
- Asparuhova MB, "A novel volume-stable collagen matrix induces changes in the behavior of primary human oral fibroblasts, periodontal ligament, and endothelial cells", Int J Mol Sci, 22, pp.4051~ (2021)
- Imber JC, "Pre-clinical evaluation of the effect of a volume-stable collagen matrix on periodontal regeneration in two-wall intrabony defects", J Clin Periodontol, 48, pp.560~569 (2021)
- Sorushanova A, "The collagen suprafamily : from biosynthesis to advanced biomaterial development", Adv Mater, 31, pp.e1801651~ (2019)
- AlSarhan MA, "Xenogeneic collagen matrix versus connective tissue graft for the treatment of multiple gingival recessions : a systematic review and meta-analysis", Clin Exp Dent Res, 5, pp.566~579 (2019)
- Yoo SY, "Periodontal healing using a collagen matrix with periodontal ligament progenitor cells in a dehiscence defect model in beagle dogs", J Periodontal Implant Sci, 49, pp.215~227 (2019)
- Wikesjö UM, "Periodontal repair in dogs : effect of rhBMP-2 concentration on regeneration of alveolar bone and periodontal attachment", J Clin Periodontol, 26, pp.392~400 (1999)
- Shirakata Y, "Healing of localized gingival recessions treated with a coronally advanced flap alone or combined with an enamel matrix derivative and a porcine acellular dermal matrix : a preclinical study", Clin Oral Investig, 20, pp.1791~1800 (2016)
- Suárez-López Del Amo F, "Comparison of two soft tissue substitutes for the treatment of gingival recession defects : an animal histological study", J Appl Oral Sci, 27, pp.e20180584~ (2019)
- Shirakata Y, "Split-mouth evaluation of connective tissue graft with or without enamel matrix derivative for the treatment of isolated gingival recession defects in dogs", Clin Oral Investig, 23, pp.3339~3349 (2019)
- Schmitt CM, "Soft tissue volume alterations after connective tissue grafting at teeth : the subepithelial autologous connective tissue graft versus a porcine collagen matrix-a pre-clinical volumetric analysis", J Clin Periodontol, 43, pp.609~617 (2016)
- Naenni N, "Volumetric and linear changes at dental implants following grafting with volume-stable three-dimensional collagen matrices or autogenous connective tissue grafts : 6-month data", Clin Oral Investig, 22, pp.1185~1195 (2018)
- Lee D, "Evaluation of regeneration after the application of 2 types of deproteinized bovine bone mineral to alveolar bone defects in adult dogs", J Periodontal Implant Sci, 52, pp.370~382 (2022)
- Miyaji H, "Influence of root dentin surface conditioning with bone morphogenetic protein-2 on periodontal wound healing in beagle dogs", J Oral Tissue Eng, 8, pp.173~180 (2011)
- Kato A, "Combination of root surface modification with BMP-2 and collagen hydrogel scaffold implantation for periodontal healing in beagle dogs", Open Dent J, 9, pp.52~59 (2015)
- Hoodless PA, "MADR1, a MAD-related protein that functions in BMP2 signaling pathways", Cell, 85, pp.489~500 (1996)
- Nishimura R, "Smad5 and DPC4 are key molecules in mediating BMP-2-induced osteoblastic differentiation of the pluripotent mesenchymal precursor cell line C2C12", J Biol Chem, 273, pp.1872~1879 (1998)
- Harrington AE, "Structural basis for the inhibition of activin signalling by follistatin", EMBO J, 25, pp.1035~1045 (2006)
- Greenwald J, "A flexible activin explains the membrane-dependent cooperative assembly of TGF-beta family receptors", Mol Cell, 15, pp.485~489 (2004)
- Gray PC, "Identification of a binding site on the type II activin receptor for activin and inhibin", J Biol Chem, 275, pp.3206~3212 (2000)
- Massagué J, "Serine", threonine kinase receptors : mediators of transforming growth factor beta family signals, 1996, pp.27, 41~64
- Allendorph GP, "Designer TGFβ superfamily ligands with diversified functionality", PLoS One, 6, pp.e26402~ (2011)
- Danesh-Meyer MJ, "Tissue engineering in periodontics using rhBMP-2", J N Z Soc Periodontol, (85), pp.10~14 (2000)
- Vignoletti F, "Clinical and histological healing of a new collagen matrix in combination with the coronally advanced flap for the treatment of Miller class-I recession defects : an experimental study in the minipig", J Clin Periodontol, 38, pp.847~855 (2011)
- Kloukos D, "Transgingival probing : a clinical gold standard for assessing gingival thickness", Quintessence Int, 52, pp.394~401 (2021)
- Couso-Queiruga E, "Assessment of gingival thickness using digital file superimposition versus direct clinical measurements", Clin Oral Investig, 25, pp.2353~2361 (2021)