본문 바로가기
KERD
논문KCI 등재

Ginsenoside Rb1이 C6 Glioma 세포주의 Nitric Oxide 생성과 세포자멸사에 미치는 효과

The Effects of Ginsenoside Rb1 on the Apoptosis and the Production of Nitric Oxide in Rat C6 Glioma Cells

2

조회

0

다운로드

3

인용

0

참고문헌

주제분류병리학

초록

Abstract

Background : Ginsenosides, the extract of Panax ginseng, exert various pharmacological effects such as anticancer activity by the mechanism that is not yet defined. In this study, we proposed that the anticancer effect of ginsenoside Rb1 is related to tumor cell apoptosis and ginsenoside Rb1 induces the tumor cell apoptosis via the nitric oxide (NO) production. Methods : Rat C6 glioma cells were activated by treating with lipopolysaccharide (LPS), interferon (IFN)- , and tumor necrosis factor (TNF)- on the culture medium to investigate the effects of ginsenoside Rb1. Results : Compared with C6 glioma cells treated with LPS/IFN- /TNF- , C6 glioma cells treated with LPS/IFN- /TNF- /ginsenoside Rb1 showed marked increase in the NO production and apoptosis. Ginsenoside Rb1 induces the NO production in C6 glioma cells in dose-dependent manner. When C6 glioma cells treated with LPS/IFN- /TNF- /ginsenoside Rb1 were incubated with the specific inhibitor of iNOS, S-Methyl-2-thiopseudoureasulfate (SMT), both NO production and apoptosis in C6 glioma cells was significantly decreased. Ginsenoside Rb1 induced the expression of iNOS mRNA and iNOS protein in C6 glioma cells. Conclusions : These results suggest that the induction of iNOS expression and subsequent

참고문헌 (27)

  1. "Ginseng pharmacology: multiple constituentsand multiple actions.", 58, pp.1685~1693 (1999)
  2. "Panax ginseng pharmacology: a nitric oxide link?", 54, pp.1~8 (1997)
  3. "Effects of ginsenosides onimpaired performance caused by scopolamine in rats", 312, pp.149~51 (1996)
  4. "influenceon neurotransmitter uptake in rat brain synaptosomes", 3, pp.221~4 (1985)
  5. "Adjuvant effect of ginsengextracts on the immune responses to immunisation against Staphylococcusaureus in dairy cattle", 91, pp.29~37 (2003)
  6. "Protection of ischemic hippocampalneurons by ginsenoside Rb1 a main ingredient of ginseng root", 28, pp.191~200 (1997)
  7. "Protective effectof ginsenosides,active ingredients of Panax ginseng on kainic acidinducedneurotoxicity in rat hippocampus", 325, pp.129~33 (2002)
  8. "Neuroprotective effects of ginseng totalsaponin and ginsenosides Rb1 and Rg1 on spinal cord neurons invitro", 173, pp.224~34 (2002)
  9. "Ginsenosides Rb1 and Rg3protect cultured rat cortical cells from glutamate-induced neurodegeneration", 53, pp.426~32 (1998)
  10. "Inhibition of apoptosis by ginsenoside Rg1 in culturedcortical neurons", 110, pp.535~9 (1997)
  11. "Protective effectof ginsenoside Rg1 on dopamine-induced apoptosis in PC12 cells", 22, pp.673~8 (2001)
  12. "Plant-glycoside modulationof cell surface related to control of differentiation in culturedB16 melanoma cells", 47, pp.3863~7 (1987)
  13. "G1phase-specific suppression of the Cdk2 activity by ginsenoside Rh2in cultured murine cells", 60, pp.39~44 (1997)
  14. "Anti-proliferative effect of ginseng saponinson human prostate cancer cell line", 67, pp.1297~306 (2000)
  15. "Induction ofapoptosis by a novel intestinal metabolite of ginseng saponin viacytochrome c-mediated activation of caspase-3 protease", 60, pp.677~85 (2000)
  16. "Inhibitoryeffects by oral administration of ginsenoside Rh2 on the growth ofhuman ovarian cancer cells in nude mice", 120, pp.24~6 (1993)
  17. "Inhibitory effect of tumormetastasis in mice by saponins,ginsenoside-Rb2 20(R)-and 20(S)-ginsenoside-Rg3, of red ginseng", 18, pp.1197~202 (1995)
  18. "Experimental and epidemiological evidence of the cancerpreventiveeffects of Panax ginseng C.A. Meyer", 54, pp.71~81 (1996)
  19. "Trial of a new medium-term model usingbenzo pyrene induced lung tumor in newborn mice", 15, pp.839~45 (1995)
  20. "Ginseng extract scavenges hydroxylradical and protects unsaturated fatty acids from decompositioncaused by iron-mediated lipid peroxidation", 20, pp.145~50 (1996)
  21. "Transcriptional activation of the Cu Zn-superoxide dismutase gene through the AP2 site by ginsenosideRb2 extracted from a medicinal plant", 271, pp.24539~43 (1996)
  22. "Ginsenosides stimulate endogenous productionof nitric oxide in rat kidney", 28, pp.573~80 (1996)
  23. "Cerebral vasodilationinduced by stimulation of the pterygopalatine ganglion and greaterpetrosal nerve in anesthetized monkeys", 96, pp.393~8 (2000)
  24. "Inflammation, free radicals, and antioxidants", 12, pp.274~7 (1996)
  25. "Activation-induced cell death of rat astrocytes", 900, pp.342~7 (2001)
  26. "Ginsenoside RH-2 induces apoptoticcell death in rat C6 glioma via a reactive oxygen- and caspase-dependentbut BCL-X(L)-independent pathway", 65, pp.33~40 (1999)
  27. "Activation of caspase-3protease via a Bcl-2-insensitive pathway during the process of ginsenosideRh2-induced apoptosis", 121, pp.73~81 (1997)

관련 논문AI추천

이 간행물의 다른 논문 (12건)