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노화초기 백서 신장에서의 Nitric Oxide Synthase 발현

Nitric Oxide Synthase Expression in Early Stage of Aging Rat Kidney

정현주|권계원
대한병리학회|2004.04|Journal of Pathology and Translational Medicine|제38권 제2호|pp. 86-92

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참고문헌

주제분류병리학

초록

Abstract

Background : Nitric oxide synthase (NOS) has been suggested to have a role in renal injury of aging rats. Methods : Renal function and histology were compared between 12 monthand 7-9 week-old rats. Proliferating activity and cell death were evaluated by PCNA index and apoptosis. Three isoforms of NOS (eNOS, iNOS, and nNOS) were stained by immunohistochemistry. Results : Serum creatinine level was increased in old rats (1.0 mg/dL vs 0.5 mg/dL, p=0.000). 24 h proteinuria and urinary NO were comparable between the two groups. The percentage of global and segmental glomerulosclerosis increased in old rats. PCNA index decreased in the glomeruli (0.1 vs 0.6/glomerulus, p=0.005) and the tubulointerstitium (10.2 vs 19.2/mm2, p=0.019) of old rats compared to that of young rats. However, no difference was observed in the number of TUNEL positive cells. eNOS was not stained in young and old rat kidney, whereas iNOS was stained in the interstitial inflammatory cells of old rats (0.3 vs 0.0 of young rats/mm2, p=0.188). Macula densa nNOS staining significantly decreased in old rats compared to young rats (5.6 vs 9.5/mm2, p=0.009). Conclusions : Proliferating activity is more affected than cell death with aging. Decreased nNOS expression without alteration of eNOS and iNOS expressions may implicate nNOS as a marker of renal injury in the early stage of aging.

참고문헌 (29)

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관련 논문AI추천

짧은 허혈과 재관류 횟수에 따라 흰쥐의 뼈대근육에서 나타나는 산화질소합성효소의 발현 변화고봉철, 박현주, 백두진, 서윤경, 이덕구, 이상완, 이완희, 장남진, 전수경대한체질인류학회 · 해부·생물인류학2006 · 제19권 제2호노화가 사람 치은섬유아세포의 nitric oxide와 inducible nitric oxide synthase 발현에 끼치는 영향지숙, 국중기, 김병옥, 김종관, 김흥중, 박주철, 장현선대한치주과학회 · Journal of Periodontal & Implant Science2006 · 제36권 제2호분화중인 흰쥐 콩팥의 요세관에서 nNOS의 발현김진, 류시윤, 송지현, 정주영한국현미경학회 · Applied Microscopy2008 · 제38권 제2호기니픽의 정상 내이에서의 NOS의 분포 양상박숙경, 정원호, 조도연, 홍성화대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2003 · 제46권 제8호백서 뇌의 정상 노화와 병적 노화 과정의 조직학적 및 약물효과길영기, 김순옥, 장희경, 전진숙, 한호성대한생물치료정신의학회 · 생물치료정신의학1997 · 제3권 제1호補中治濕湯의 백서 신장 수분채널, Na, K-ATPase, 산화질소 합성효소 발현에 미치는 영향강대길, 김복해, 김종균, 류도곤, 손은진, 이호섭, 조동기한의병리학회 · 동의생리병리학회지2002 · 제16권 제1호

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