B형 미만성 대세포 림프종에서 Smad 단백의 발현
Expression Pattern of Smad Proteins in Diffuse Large B-cell Lymphomas
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Abstract
Background : Smad proteins mediate the cellular signaling from members of transforming growth factor-β family (TGF-βs). Smads 2 and 3 transmit signals from TGF-β and activin, and Smads 1, 5, and 8 transmit signals from the bone morphogenetic protein. Smad4 is known to be a common mediator of both pathways, yet little is known about the expression pattern of Smad proteins in normal lymphoid tissue and malignant lymphoma. Methods : Immunohistochemistry was performed for Smad3 and Smad4 on the paraffin-embedded tissue sections from 32 cases of diffuse large B-cell lymphomas. Results : In reactive lymphoid tissue, nearly all cells of the germinal centers were positive for Smad3 and more than 50% of paracortical cells were positive for Smad3. For Smad4 immunostaining, nearly all cells of the germinal centers showed diffuse cytoplasmic staining, and most of them revealed nuclear positivity as well. Most of the cells in the paracortex regions were positive for Smad4. For the malignant lymphomas, all the cases were positive for Smad3, but 26 cases were positive for Smad4 and 6 cases (19%) were negative for Smad4. Conclusions : These results suggest that TGF-β-specific Smads may be actively involved for signal transduction in lymphoid organs, and the TGF-β signaling pathway through Smads is operative in malignant lymphoma. The loss of Smad4 expression might be associated with development of some diffuse large B-cell lymphomas.
참고문헌 (17)
- "The Smad4 activationdomain (SAD) is a proline-rich, p300-dependent transcriptional activationdomain.", 275, pp.2115~2122 (2000)
- "Immunohistochemical expressionof Smads 1-6 in the 15-day gestation mouse embryo: signalingby BMPs and TGF-betas.", 220, pp.141~154 (2001)
- "Selective loss of thetransforming growth factor-beta apoptotic signaling pathway inmutant NRP-154 rat prostatic epithelial cells", 11, pp.1~10 (2000)
- "Smad7is induced by CD40 and protects WEHI 231 B-lymphocytes fromtransforming growth factor-beta -induced growth inhibition andapoptosis", 275, pp.38363~38370 (2000)
- "TGF-beta receptors and signal transduction.", pp.97~104 (1997)
- "A deletionin the gene for transforming growth factor beta type I receptorabolishes growth regulation by transforming growth factor beta ina cutaneous T-cell lymphoma", 94, pp.2854~2861 (1999)
- "Loss of receptors for transforming growth factor beta in humanT-cell malignancies", pp.6002~6006 (1994)
- "A dominant inhibitorymutant of the type II transforming growth factor beta receptor in themalignant progression of a cutaneous T-cell lymphoma.", 16, pp.3480~3489 (1996)
- "Intestinal tumorigenesis in compound mutant mice of both Dpc4(Smad4)and Apc genes.", 92, pp.645~656 (1998)
- "Smad3 mutant micedevelop metastatic colorectal cancer.", 94, pp.703~714 (1998)
- "Cyclin Adown-regulation in TGFbeta1-arrested follicular lymphoma cells.", 261, pp.250~259 (2000)
- "Abundant expression of transforminggrowth factor-beta 1 and -beta 2 by Hodgkin’s Reed-Sternbergcells and by reactive T lymphocytes in Hodgkin’s disease", pp.249~55
- "Decreased expression of TGF- type 2 receptor in primaryB-cell lymphomas of the stomach,", 198, pp.333~337 (2002)
- "Immunohistochemicalevidence of a role for transforming growth factor betain the pathogenesis of nodular sclerosing Hodgkin’s disease", 136, pp.1209~1214 (1990)
- "Transforming growth factor-beta signaling in normaland malignant hematopoiesis.", 17, pp.1731~1737 (2003)
- "Transforming growth factor: signal transductionpathways, cell cycle mediation, and effects on hematopoiesis.", 10, pp.67~74 (2001)
- "Targeted disruption of SMAD3results in impaired mucosal immunity and diminished T cell responsiveness", 18, pp.1280~1291 (1999)