대장암에서 M30 면역 염색으로 검출된 세포 자멸사와 MSI및 임상 병리학적 특징과의 상관 관계
Assessment of Apoptosis by M30 Immunoreactivity and the Relationship with the MSI status and the Clinicopathological Characteristics of Colorectal Carcinomas
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Abstract
Background : The monoclonal antibody M30 recognizes a neoepitope of cytokeratin 18 that’s produced during the process of apoptosis, and it is reactive in formalin-fixed, paraffin-embedded tissue. The detailed nature of apoptosis in colorectal cancer is unclear, especially in regard to the MSI status and the clinicopathologic factors. The purpose of this study was to elucidate the apoptosis assessed by M30 immunoreactivity in colorectal cancer and its relationship with the MSI status and the various clinicopathologic factors of colorectal cancers. Methods : 101 colorectal cancers were classified according to levels of MSI as 12 MSI-H, 4 MSI-L and 85 MSS. Apoptosis was quantified by immunohistochemistry with using M30 CytoDEATH antibody. Results : The apoptotic index assessed by M30 was significantly increased in the MSIH and MSI-L colorectal cancer compared to that in the MSS colorectal cancer. Right sided colon cancer showed a significant higher apoptotic index than did the left sided colon cancer. There was also a tendency for decreased apoptosis in metastatic colorectal cancers (Duke’s stage D). There was somewhat of an increase of apoptosis in colorectal cancers with mucinous carcinoma and medullary carcinoma, and also in the colorectal cancers with an increased TIL count, but this was not statistically significant. Conclusion : M30 immunoreactivity is a valuable method to detect apoptosis in formalin-fixed, paraffin-embedded tissue and it might explain that MSI-H colorectal cancer shows better clinical behavior than MSS colorectal cancer in regard to the increased apoptosis.
참고문헌 (30)
- "Apoptosis in the pathogenesis and treatment of disease", 267, pp.1456~1462 (1995)
- "Review article:manipulation of cell death--the development of novel strategies for the treatment of gastrointestinal disease", 9, pp.215~226 (1995)
- "Apoptosis:a basic biological phenomenon with wide-ranging implications in tissue kinetics", 26, pp.239~257 (1972)
- "Changes in apoptosis during the development of colorectal cancer:a systematic review of the literature", 45, pp.37~53 (2003)
- "Quantitative differentiation of both free 3’-OH and 5’-OH DNA ends between heat-induced apoptosis and necrosis", 46, pp.1051~1059 (1998)
- "Caspase activation during spontaneous and radiation-induced apoptosis in the murine intestine", 195, pp.285~292 (2001)
- "Fractin immunostaining for the detection of apoptotic cells and apoptotic bodies in formalin-fixed and paraffin-embedded tissue", 79, pp.619~620 (1999)
- "Immunocytochemical detection and mapping of a cytokeratin 18 neo-epitope exposed during early apoptosis", 187, pp.567~572 (1999)
- "M30 expression demonstrates apoptotic cells,correlates with in situ end-labeling,and is associated with Ki-67 expression in large intestinal neoplasms", 124, pp.1768~1772 (2000)
- "Microsatellite instability in colorectal cancer:different mutator phenotypes and the principal involvement of hMLH1", 58, pp.1713~1718 (1998)
- "Morphology of sporadic colorectal cancer with DNA replication errors", 42, pp.673~679 (1998)
- "Characterization of a subtype of colorectal cancer combining features of the suppressor and mild mutator pathways", 52, pp.455~460 (1999)
- "Tumour infiltrating lymphocytes and apoptosis are independent features in colorectal cancer stratified according to microsatellite instability status", 48, pp.360~366 (2001)
- "Value of histopathology in predicting microsatellite instability in hereditary nonpolyposis colorectal cancer and sporadic colorectal cancer", 27, pp.1407~1417 (2003)
- "Tumor microsatellite instability and clinical outcome in young patients with colorectal cancer", 342, pp.69~77 (2000)
- "Microsatellite instability in sporadic mucinous colorectal carcinomas:relationship to clinicopathological variables", 182, pp.380~384 (1997)
- "Mismatch repair proficiency and in vitro response to 5-fluorouracil", 117, pp.123~131 (1999)
- "The role of DNA mismatch repair in drug resistance", 4, pp.1~6 (1998)
- "Apoptosis induced by overexpression of hMSH2 or hMLH1", 59, pp.3021~3027 (1999)
- "DNA mismatch repair genes and colorectal cancer", 47, pp.148~153 (2000)
- "High prevalence of activated intraepithelial cytotoxic T lymphocytes and increased neoplastic cell apoptosis in colorectal carcinomas with microsatellite instability", 154, pp.1805~1813 (1999)
- "In situ end-labelling detects DNA strand breaks in apoptosis and other physiological and pathological states", 170, pp.1~8 (1993)
- "Caspase cleavage of keratin 18 and reorganization of intermediate filaments during epithelial cell apoptosis", 138, pp.1379~1394 (1997)
- "The impact of microsatellite instability on the molecular phenotype of colorectal tumors", 63, pp.4577~4582 (2003)
- "Methylation of O-6-methylguanine DNA methyltransferase characterizes a subset of colorectal cancer with low-level DNA microsatellite instability", 61, pp.827~830 (2001)
- "Frequency of apoptosis relates inversely to invasiveness and metastatic activ-ity in human colorectal cancer", 431, pp.241~248 (1997)
- "Apoptosis as a prognostic factor in colorectal carcinoma", 28, pp.145~150 (1998)
- "Apoptosis of colon cancer:comparison with Ki-67 proliferative activity and expression of p53", 122, pp.166~170 (1996)
- "Pathological and genetic correlates of apoptosis in the progression of colorectal neoplasia", 18, pp.146~156 (1997)
- "Apoptosis and metastasis:increased apoptosis resistance of metastatic cancer cells is associated with the profound deficiency of apoptosis execution mechanisms", 115, pp.185~193 (1997)