내분비계 장애물질에 의한 고환 내 세포손상에서 아포토시스 관련 분자들의 변화
The Apoptotic Molecular Changes of Cellular Injury in Mouse Testis Induced by Endocrine Disrupting Chemicals
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Abstract
Background : Spermatogenesis is regulated by various cellular reactions, and especially cell proliferation and apoptosis. Methods : We investigated the morphological changes and the apoptotic molecular changes in mouse testis induced by the endocrine disrupting chemicals. ICR mice were treated with bisphenol A (BPA), 2-bromopropane (2-BP) and diethylstilbesterol (DES). Histological examination and immunohistochemical staining, TUNNEL staining and RNAse protection assay were conducted. Results : Testes treated with BPA showed normal spermatogenesis and the proliferation activity, and the density of the cells was similar with those in the control. 2-BP and DES groups, which showed a decrease of germ cells near the basal layer and degenerative changes. The proliferative activity identified by PCNA staining was significantly decreased in the 2-BP and DES groups (p<0.05). The apoptosis was significantly increased in the 2-BP group however, a significant decrease was noted in the BPA group (p<0.05). Among apoptosis-related molecules, the expression of Fas, Fas ligand, TRAIL, TNFp55 and caspase 1, 3, 6 and 8 were changed according to the change of the degree of apoptosis in all groups. Conclusions : Endocrine disrupting chemicals induced cellular injury in mouse testis through the changes of proliferative activity and apoptosis which was regulated by a number of apoptosis-related molecules. This probably results in the abnormality of spermatogenesis in mouse testis.
참고문헌 (20)
- "Are estrogens carcino-genic during development of the testes?", 106, pp.240~242 (1998)
- "Hematopoietic and reproductive toxicity of2-bromopropane,a recently introduced substitute for chlorofluoro-carbons", 108, pp.309~313 (1999)
- "Genetic vari-ation in susceptibility to endocrine disruption by estrogen in mice", 285, pp.1259~1261 (1999)
- "Exposure with the environmental estrogen bisphenol A disruptsthe male reproductive tract in young mice", 65, pp.2351~2357 (1999)
- "A role for estrogens in the malereproductive system.", 390, pp.509~512 (1997)
- "Targeted disruption of theestrogen receptor gene in male mice causes alteration of spermato-genesis and infertility.", 137, pp.4796~4805 (1996)
- "Effects of bisphenol Aon adult male mouse fertility.", 110, pp.163~167 (2002)
- "Bisphenol A inhibitstesticular functions and increases luteinizing hormone secretion inadult male rats.", 226, pp.216~221 (2001)
- "Mechanism of chronic obstruc-tive uropathy :increased expression of apoptosis-promoting mole-cules,", 58, pp.1481~1491 (2000)
- "A decade of caspases", pp.8543~8567 (2003)
- "Diethylstilbestrol induces rat spermatogenic cell236 왕은휘이권행양기화 외 4인apoptosis in vivo through increased expression of spermatogeniccell Fas", FasL system, 2003, pp.278, 6470~6481
- "Involvement of Bcl-2 family genesand Fas signaling system in primary and secondary male germ cellapoptosis induced by 2-bromopropane in rat", 174, pp.35~48 (2001)
- "Intrauterinebisphenol A exposure leads to stimulatory effects on Sertolicell number in rats.", 91, pp.95~103 (2003)
- "The occupational exposurelevel (OEL) for 2-bromopropane: the first OEL established byKorea.", 14, pp.356~358 (1999)
- "Effects of 2-bromopropaneon spermatogenesis in the Sprague-Dawley rat", 13, pp.179~187 (1999)
- "Dietarydiethylstilbestrol but not genistein adversely affects rat testiculardevelopment1,2,", 133 (2003)
- "Time- and dose-relatedeffects of estradiol and diethylstilbestrol on the morphology and functionof the fetal rat testis in culture", pp.160~169 (2003)
- "Leydig cell apoptosis in the rat testes after administrationof the cytotoxin ethane dimethanesulphonate role of the Bcl-2 familymembers", 157, pp.317~326 (1998)
- "Caspase-3 activation in required for Leydigcell apoptosis induced by ethane dimethanesulfonate", 142, pp.1846~1853 (2000)
- "A molecular titration assay to measure transcriptprevalence levels", pp.633~48