Expression of p63 and its Isoform, deltaNp63, in Non-Small Cell Lung Carcinoma
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Background : Several studies have been conducted on the role of the p63 gene family in nonsmall cell lung carcinoma (NSCLC). Nevertheless, the role of these genes in the development and progression of NSCLC remains controversial. This study was designed to examine the expression and clinicopathologic significance of the p63 family in NSCLC. Methods : Immunohistochemical staining was performed on 92 cases of NSCLC (47 squamous cell carcinomas [SqCCs] and 45 adenocarcinomas [ACs]) using tissue microarray blocks. The results were analyzed and correlated with clinicopathologic data. Results : The expression of deltaNp63 (△Np63) was elevated in SqCC (39/47), but not in AC (2/45; p<0.01). Both p63 and Np63 had high expression in 39 SqCCs; p63 and △Np63 also had a similar geomorphologic distribution in most positive tumors. The expression of △Np63 was correlated with histologic type, gender, pT stage, p53 expression, and p63 expression. pT and pN stages were independent factors in survival (p<0.05, respectively). Conclusions : The major p63 isoform in NSCLC, △Np63, had a strong correlation with p53 and p63, and was exclusively expressed in SqCC. However, our findings suggest that △Np63 was not an independent prognostic factor for NSCLC.
Abstract
Background : Several studies have been conducted on the role of the p63 gene family in nonsmall cell lung carcinoma (NSCLC). Nevertheless, the role of these genes in the development and progression of NSCLC remains controversial. This study was designed to examine the expression and clinicopathologic significance of the p63 family in NSCLC. Methods : Immunohistochemical staining was performed on 92 cases of NSCLC (47 squamous cell carcinomas [SqCCs] and 45 adenocarcinomas [ACs]) using tissue microarray blocks. The results were analyzed and correlated with clinicopathologic data. Results : The expression of deltaNp63 (△Np63) was elevated in SqCC (39/47), but not in AC (2/45; p<0.01). Both p63 and Np63 had high expression in 39 SqCCs; p63 and △Np63 also had a similar geomorphologic distribution in most positive tumors. The expression of △Np63 was correlated with histologic type, gender, pT stage, p53 expression, and p63 expression. pT and pN stages were independent factors in survival (p<0.05, respectively). Conclusions : The major p63 isoform in NSCLC, △Np63, had a strong correlation with p53 and p63, and was exclusively expressed in SqCC. However, our findings suggest that △Np63 was not an independent prognostic factor for NSCLC.
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