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논문KCI 등재

안면신경 압박손상 후 Adenovirus 매개 BDNF 유전자 전달을 통한 신경손상 회복에 관한 연구

Adenoviral vector mediated in vivo gene transfer of BDNF promotefunctional recovery after facial nerve crush injury

이종호|양병은
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2006.08|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제32권 제4호|pp. 308-316

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참고문헌

주제분류치의학

초록

Abstract

Objectives Despite considerable advances in technique, experience and skill, the precise place of surgery in the treatment of facial nerve injury remains uncertain. We designed a facial nerve crush injury model in rats and evaluated the recovery of crushed nerve which is the most common injury type of facial nerve using adenovirus vector mediated in vivo gene transfer of Brain derived neurotrophic factor(BDNF). Materials and methods In 48 Sprague Dawley rats, we made a facial nerve crush injury model to main trunk before the furcation, and injected a 1011pfu adenoviral BDNF in experimental group(BDNF adenoviral injection group; ad-BDNF) and 3㎕ saline in control group(Saline injection group; saline). After a period of regeneration from 10 to 40 days, nerve regeneration was evaluated with functioinal test (vibrissae and ocular movement), electrophysiologic study(threshold, peak voltage, conduction velocity) and histomorphometric study of axon density. Results Vibrissae and ocular movement, threshold and conduction velocity improved as time elapse in both group, however axon density was increased significantly only in experimental group. Functional test in 10 days and 20 days showed no difference between experimental group and control group. Vibrissae movement, threshold, conduction velocity and axon density in 30 days revealed that the regeneration in quality of experimental group was significantly superior to that of control group. Conclusion In general, there is tendency for nerve regeneration in experimental group (BDNF-adenovirus injection group) during 40 days, functional recovery was detected successfully after facial nerve crush in 30 days postoperatively.

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손상된 얼굴신경의 재생에 관한 고찰박문성, 박영석, 백기석, 이승표, 장미숙대한구강해부학회 · 대한구강해부학회지2012 · 제33권 제1호인회석 박막 피복 도관과 Brain-derived neurotrophic factor(BDNF) 유전자 이입 슈반세포를 이용한 백서 좌골신경 재생에 관한 연구김남열, 김명진, 김성민, 김성준, 김윤희, 김중수, 김현만, 안강민, 양병은, 오승재, 유상배, 이종호, 장정원, 정필훈, 최원재, 황순정대한구강악안면외과학회 · 대한구강악안면외과학회지2005 · 제31권 제3호BDNF 유전자 이입 슈반세포와 PGA 도관을 이용한 백서 좌골신경 재생에 관한 연구고은봉, 김남열, 김명진, 김병수, 김성민, 김윤태, 김윤희, 신영민, 안강민, 이종호, 장정원, 조승우, 최원재, 황순정대한구강악안면외과학회 · 대한구강악안면외과학회지2004 · 제30권 제6호Changes in Expression of High Voltage-Activated Calcium Channel in Facial Motoneurons Following Facial Nerve Injury오석배대한구강생물학회 · International Journal of Oral Biology2004 · 제29권 제4호급성 안면신경 손상 모델에서 혈소판 풍부 혈장의 신경재생 효과김송희, 박종성, 이경화, 이상철, 장수정, 정한성, 조용범, 조형호, 한재영대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2009 · 제52권 제6호안면신경 손상 후 안면신경 원위부에서 톨 유사 수용체(Toll-Like Receptor) Messenger Ribonucleic Acid의 발현 양상김상훈, 김영일, 민혜규, 여승근, 이재민, 정준양대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2022 · 제65권 제8호

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