다형성 선종과 선양낭성 암종에서 상피성장인자 발현에 관한 연구
The study of EGF expression between human pleomorphic adenoma and adenoid cystic carcinoma
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Epidermal growth factor is a single-chain polypeptide consisting of 53 amino acids and has a potent mitogenic activity that stimulates proliferation of various normal and neoplastic cells through the interaction with its specific receptor(epidermal growth factor receptor, EGFR). Pleomorphic adenoma is the most common salivary benign tumor and histologically, it contains the epithelial cell, the myo-epithelial cell and mesenchymal ingredient, which is various aspect. Adenoid cystic carcinoma is an infiltrative malignant salivary gland tumor with three different histological patterns: cribriform, tubular or solid. The tumor cell structure composed of modified myoepithelial cell, and basaloid cell. In this study, we used an immunohistochemical technique to investigate the expression of EGF in 6 specimens of adenoid cystic carcinoma and 10 specimens of pleomorphic adenoma taken from patients treated at Dept. of Oral and Maxillofacial Surgery, Dankook University. The results were as follows. 1. In pleomorphic adenoma, ductal structure and scattered spindle cells in hyalinized stroma, disclosing myxoid stroma and hyalin, cartilage formation were observed. Immunohistologically, weak EGF expression in ductal structure and negative in stromal area were observed. 2. Cribriform type of adenoid cystic carcinoma showed numerous pseudocyst surrounded by dark small neoplastic cells in the back-ground of fibrous connective tissue and moderate EGF expression of dark cells adjacent to pseudo lumen in cribriform pattern, while weak expression in other most cells. 3. Tubular type of adenoid cystic carcinoma showed numerous ductal pattern surrounded by two layered neoplastic cells in the back-ground of fibrous connective tissue and strong EGF expression in luminal cells of ductal structure, while weak expression in outer cells. From the results obtained, we suggest that EGF is mainly biosynthesized in cells forming duct like structures of tubulo-ductal type or cribriform adenoid cystic carcinoma and it may play a role, as a cell mitogen in adenoid cystic carcinoma growth.
Abstract
Epidermal growth factor is a single-chain polypeptide consisting of 53 amino acids and has a potent mitogenic activity that stimulates proliferation of various normal and neoplastic cells through the interaction with its specific receptor(epidermal growth factor receptor, EGFR). Pleomorphic adenoma is the most common salivary benign tumor and histologically, it contains the epithelial cell, the myo-epithelial cell and mesenchymal ingredient, which is various aspect. Adenoid cystic carcinoma is an infiltrative malignant salivary gland tumor with three different histological patterns: cribriform, tubular or solid. The tumor cell structure composed of modified myoepithelial cell, and basaloid cell. In this study, we used an immunohistochemical technique to investigate the expression of EGF in 6 specimens of adenoid cystic carcinoma and 10 specimens of pleomorphic adenoma taken from patients treated at Dept. of Oral and Maxillofacial Surgery, Dankook University. The results were as follows. 1. In pleomorphic adenoma, ductal structure and scattered spindle cells in hyalinized stroma, disclosing myxoid stroma and hyalin, cartilage formation were observed. Immunohistologically, weak EGF expression in ductal structure and negative in stromal area were observed. 2. Cribriform type of adenoid cystic carcinoma showed numerous pseudocyst surrounded by dark small neoplastic cells in the back-ground of fibrous connective tissue and moderate EGF expression of dark cells adjacent to pseudo lumen in cribriform pattern, while weak expression in other most cells. 3. Tubular type of adenoid cystic carcinoma showed numerous ductal pattern surrounded by two layered neoplastic cells in the back-ground of fibrous connective tissue and strong EGF expression in luminal cells of ductal structure, while weak expression in outer cells. From the results obtained, we suggest that EGF is mainly biosynthesized in cells forming duct like structures of tubulo-ductal type or cribriform adenoid cystic carcinoma and it may play a role, as a cell mitogen in adenoid cystic carcinoma growth.
참고문헌 (30)
- J. Phillip Sapp et al, "Contemporary Oral and Maxillofacial pathology", pp.336~355 (1997), Mosby
- John E, "Color atlas and text of the salivary glands disease, disorders and surgery", Mosby-Wolfe, pp.63~65 (1995)
- Batasakis JG, "Histopathologic grading of salivarary gland neoplasm: adenoid cystic carcinomas", Ann Oto Rhinol Laryngol, 99, pp.1007~1009 (1990)
- Brookstone MS, "Central adenoid cystic carcinoma of mandible", J Oral Maxillofacial Surg, 48, pp.1329~1333 (1990)
- Adenoid cystic carcinoma of salivary glands: a study of 61 cases with clinicopathologic correlation, , Cancer, 57, pp.312~319 (1986)
- Perzin KH, "Adenoid cystic carcinoma arising in salivary glands: a correlation of histological features and clinical courses", Cancer, 42, pp.265~282 (1978)
- Szanto PA, "Histologic gradig of adenoid cystic carcinomas of the salivary gland", Cancer, 54, pp.1062~1069 (1984)
- Cohen S, "Isolation of submaxillary gland protein accelerating incisor eruption and eyelid opening in the newborn animal", J Biol Chem, 237, pp.1544~1562 (1962)
- Cohen S, "Human epidermal growth factor: isolation and chemical and biological properties", Proc Natl Acad Sci USA, 72, pp.1317~1321 (1975)
- Ishitoya J, "Gene amplification and overexpression of EGF receptor in squamous cell carcinomas of the head and neck", Br J Cancer, 59, pp.559~562 (1989)
- Christensen ME, "Epidermal growth factor receptor expression on oral mucosa dysplastic epithelia and squamous cell carcinomas", Eur Arch Otorhinolaryngol, 249, pp.243~247 (1992)
- Shirasuna K, "Immunohistochemical localization of epidermal growth factor (EGF) and EGF receptor in human oral mucosa and its malignancy", Virchows Archiv A Pathol Anat, 418, pp.349~353 (1991)
- James R, "Polypeptide growth factors", Ann Rev Biochem, 53, pp.259~292 (1984)
- Modjtahedi H, "Phase I trial and tumor localization of the anti-EGFR monoclonal antibody ICR62 in head and neck or lung cancer", Br J Cancer, 73, pp.228~235 (1996)
- Carpenter G, "Epidermal growth factor, its receptor, and related proteins", Experim Cell Res, 164, pp.1~10 (1986)
- Ino M, "Kinetics of epidermal growth factor in saliva", Acta Otolaryngol(Stockh) Suppl, 500, pp.126~130 (1993)
- Gardner DP, "Loss of cytotoxic effect of epidermal growth factor(EGF) on EGF receptor overexpressing cells is associated with attenuation of EGF receptor tyrosine kinase activity", J Cellular Physiol, 158, pp.245~255 (1994)
- Ozanne B, "Over-expression of the EGF receptor is a hallmark of squamous cell carcinomas", J Pathol, 149, pp.9~14 (1986)
- Kunikata M, "Epidermal growth factor receptor in squamous cell carcinoma and other epidermal lesions of squamous origin - an immunohistochemical study -", Acta Histochem Cytochem, 25, pp.387~394 (1992)
- Pusztai L, "Review article, Growth factors: Regulation of normal and neoplastic growth", J Pathol, 169, pp.191~201 (1993)
- Tahara E, "Human epidermal growth factor in gastric carcinoma as a biologic marker of high malignancy", Jpn J Cancer Res, 77, pp.145~152 (1986)
- Mori M, "Immunohistochemical distribution of human epidermal growth factor in salivary gland tumors. Virchows Arch", A Pathol Anat, 411, pp.499~507 (1987)
- Ellis GL, "Surgical Pathology of the Salivary Glands" (1991), Philadelphia, WB Saunders
- "1984", 403
- Toida M, "Histochemical studies on peudocysts in adenoid cystic carcinoma of the human salivary gland", Histochem J, 17, pp.913~924 (1985)
- Caseliz J, "Adenoid cystic carcinoma of the salivary glands: an immunohistochemical study", J Oral Pathol, 5, pp.308~318 (1986)
- Azumi N, "The cellular composition of adenoid cystic carcinoma. An immunohistochemical study", Cancer, 60, pp.1589~1598 (1987)
- Saku T, "A comparative study on the immunolocalization of keratin and myosin in salivary gland tumors", Acta Pathol Jpn, 4, pp.1031~1040 (1984)
- Busttil A, "Adenoid cystic carcinoma of minor salivary glands", J Laryngol Otol, 91, pp.41~53 (1977)
- Mochomovitz LE, "Adenoid cystic carcinoma of the salivaray gland and its histologic variants", Oral Surg Oral Med Oral Pathol, 44, pp.394~404 (1977)