Immunohistochemical study on the expression of matrix metalloproteinase 2 and high-risk human papilloma virus in the malignant progression of papillomas
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Objectives: Papilloma frequently develops as a benign tumor of the head and neck area, but its potential for malignant transformation has yet to be studied. This study aims to provide basic information for papillomas using the immunohistochemical staining of matrix metalloproteinase 2 (MMP-2) and human papilloma virus (HPV) 16 and 18. Materials and Methods: To evaluate the malignant transformation of papillomas, the selected tissue samples were serially diagnosed with pre-cancerous papilloma (with epithelial dysplasia, pseudo-epitheliomatous hyperplasia) or malignant lesion (squamous cell carcinoma, SCC) after the first diagnosis (squamous papilloma, inverted papilloma). The selected tissues were stained with an antibody to MMP-2 and HPV 16-E7, HPV 18-L1. A statistical analysis was performed according to each transformation step. Results: The epithelial layer of papilloma and pre-cancerous papilloma lesions had a similar MMP-2 expression, but that of the malignant lesion had a significantly increased MMP-2 expression. HPV 16 and 18 infection rates were 28.6%, 33.3% and 63.6% in papillomas, pre-cancerous papilloma lesions,and SCC. Conclusions: A relatively high MMP-2 expression and HPV 16 or 18 infection of papillomas may be associated with early events in the multistep processes of malignant transformation of papillomas.
Abstract
Objectives: Papilloma frequently develops as a benign tumor of the head and neck area, but its potential for malignant transformation has yet to be studied. This study aims to provide basic information for papillomas using the immunohistochemical staining of matrix metalloproteinase 2 (MMP-2) and human papilloma virus (HPV) 16 and 18. Materials and Methods: To evaluate the malignant transformation of papillomas, the selected tissue samples were serially diagnosed with pre-cancerous papilloma (with epithelial dysplasia, pseudo-epitheliomatous hyperplasia) or malignant lesion (squamous cell carcinoma, SCC) after the first diagnosis (squamous papilloma, inverted papilloma). The selected tissues were stained with an antibody to MMP-2 and HPV 16-E7, HPV 18-L1. A statistical analysis was performed according to each transformation step. Results: The epithelial layer of papilloma and pre-cancerous papilloma lesions had a similar MMP-2 expression, but that of the malignant lesion had a significantly increased MMP-2 expression. HPV 16 and 18 infection rates were 28.6%, 33.3% and 63.6% in papillomas, pre-cancerous papilloma lesions,and SCC. Conclusions: A relatively high MMP-2 expression and HPV 16 or 18 infection of papillomas may be associated with early events in the multistep processes of malignant transformation of papillomas.
참고문헌 (25)
- Sapp JP, "Contemporary oral and maxillofacial pathology" (1997), Mosby
- Underbrink MP, "Viral interaction: a possible contributing factor in head and neck cancer progression", Acta Otolaryngol, 128, pp.1361~1369 (2008)
- Atula S, "Human papillomavirus and Epstein-Barr virus in epithelial carcinomas of the head and neck region", Anticancer Res, 17, pp.4427~4433 (1997)
- Umudum H, "Human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomas", Cancer, 105, pp.171~177 (2005)
- Capone RB, "Detection and quantitation of human papillomavirus (HPV) DNA in the sera of patients with HPV-associated head and neck squamous cell carcinoma", Clin Cancer Res, 6, pp.4171~4175 (2000)
- Gillison ML, "Evidence for a causal association between human papillomavirus and a subset of head and neck cancers", J Natl Cancer Inst, 92, pp.709~720 (2000)
- Scheckenbach K, "p53 codon 72 polymorphic variants, loss of allele-specific transcription, and human papilloma virus 16 and", or 18 E6 messenger RNA expression in squamous cell carcinomas of the head and neck, 2004, pp.13, 1805~1809
- Kähäri VM, "Matrix metalloproteinases and their inhibitors in tumour growth and invasion", Ann Med, 31, pp.34~45 (1999)
- Mook OR, "The role of gelatinases in colorectal cancer progression and metastasis", Biochim Biophys Acta, 1705, pp.69~89 (2004)
- Roomi MW, "Modulation of human renal cell carcinoma 786-0 MMP-2 and MMP-9 activity by inhibitors and inducers in vitro", Med Oncol, 23, pp.245~250 (2006)
- Thomas GT, "Matrix metalloproteinases and oral cancer", Oral Oncol, 35, pp.227~233 (1999)
- 김문기, "자극성 섬유종, 구강백반증 및 구강편평세포암종에서 MMP-2 및MMP-9 발현에 대한 면역조직화학적 연구", 대한구강악안면외과학회지, 32(4), pp.352~359 (2006), 대한구강악안면외과학회
- da Silva Cardeal LB, "Higher expression and activity of metalloproteinases in human cervical carcinoma cell lines is associated with HPV presence", Biochem Cell Biol, 84, pp.713~719 (2006)
- Katori H, "Increased expression of matrix metalloproteinase-2 and 9 and human papilloma virus infection are associated with malignant transformation of sinonasal inverted papilloma", J Surg Oncol, 93, pp.80~85 (2006)
- Chambers AF, "Changing views of the role of matrix metalloproteinases in metastasis", J Natl Cancer Inst, 89, pp.1260~1270 (1997)
- Curran S, "Matrix metalloproteinases in tumour invasion and metastasis", J Pathol, 189, pp.300~308 (1999)
- Davies B, "Activity of type IV collagenases in benign and malignant breast disease", Br J Cancer, 67, pp.1126~1131 (1993)
- Tokuraku M, "Activation of the precursor of gelatinase A", 72 kDa type IV collagenase, Int J Cancer, pp.1995, 64[DOI]
- Duffy MJ, "Metalloproteinases: role in breast carcinogenesis, invasion and metastasis", Breast Cancer Res, 2, pp.252~257 (2000)
- Jones JL, "Control of matrix metalloproteinase activity in cancer", J Pathol, 183, pp.377~379 (1997)
- Gissmann L, "Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma", J Virol, 44, pp.393~400 (1982)
- IARC Working Group on the Evaluation of Carcinogenic Risks to Human, "Human papillomaviruses", IARC Monogr Eval Carcinog Risks Hum, 64, pp.1~378 (1995)
- Arndt O, "High-risk HPV types in oral and laryngeal papilloma and leukoplakia", Laryngorhinootologie, 76, pp.142~149 (1997)
- Miller CS, "Human papillomavirus expression in oral mucosa, premalignant conditions, and squamous cell carcinoma: a retrospective review of the literature", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 82, pp.57~68 (1996)
- Miller CS, "Human papillomavirus as a risk factor for oral squamous cell carcinoma: a meta-analysis, 1982-1997", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 91, pp.622~635 (2001)