본문 바로가기
KERD
논문KCI 등재

섬유모세포의 종류에 따른 불멸화된 구강 각화세포의 삼차원적 배양에 관한 연구

Role of fibroblasts in organotypic cultures of immortalized human oral keratinocytes

2

조회

0

다운로드

0

인용

0

참고문헌

주제분류치의학

초록

Abstract

Objective: In organotypic culture of immortalized human oral keratinocytes (IHOK), the change of the growth and differentiation was investigated according to the fibroblast type and the involvement of mitogen-activated protein (MAP) kinase. Materials & Methods: IHOK was cultured three dimensionally with gingival fibroblast (GF), dermal fibroblast (DF) and immortalized gingival fibroblast (IGF). We characterized biologic properties of three dimensionally reconstructed IHOK by histological, immunohistochemical, and Western blot analysis. We also investigated whether MAP kinase pathway was involved in epithelial-mesenchymal interaction by Western blot analysis. Results: The best condition of three dimensionally cultured IHOK was the dermal equivalent consisting of type I collagen and IGF. IGF increased the expression of more proliferating cell nuclear antigen (PCNA), involucrin than GF and DF in response to co-culture with IHOK. Extracellularly regulated kinase (ERK) pathway was activated in organotypic co-culture with IGF. Conclusion: The organotypic co-culture of IHOK with dermal equivalent consisting of type I collagen and IGF resulted in excellent morphologic and immunohistochemical characteristics and involved ERK pathway. The epithelial-mesenchymal interaction was activated according to the fibroblast type.

참고문헌 (36)

  1. "Multiple primary cancer in patients with cancer of the head and neck Second cancer of the head and neck", pp.467~476
  2. "HPV immortalization of human oral cells: a model for carcinogenesis", 226, pp.164~169 (1996)
  3. "The progression of head and neck cancer in vitro model", 126, pp.1313~1318 (2000)
  4. "Serial cultivation of strains of human epidermal keratinocytes the formation of keratinizing colonies from single cells. Cell 1975", pp.331~343
  5. "and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6 sulfate substrate. Surgery 1988", pp.421~431
  6. "Fibroblast behavior on gels of type I", pp.95~104
  7. "In-vitro models of stroma-dependent lymphopoiesis", 7, pp.169~177 (1995)
  8. "origins and applications in cancer reserch. Cancer Cell 1991", pp.86~92
  9. "Sponge-gel supported histoculture drug response assay for head and neck cancer. Correlations with clinical response to cisplatin. Arch Otolaryngol Head Neck Surg 1994", pp.288~292
  10. "Drug response of head and neck tumors in native-state histoculture. Arch Otolaryngol Head Neck Surg 1991", pp.83~86
  11. "Mesenchymemediated and endogenous regulation of growth and differentiation of human skin keratinocytes derived from different body sites.Differentiation 1990", pp.150~161
  12. "Mutual induction of growth factor gene expression by epidermal cell interaction. J Cell Biol 1993", pp.417~429
  13. "Clonal heterogeneity in fibroblast phenotype implications for the control of epithelial-mesenchymal interactions. Bioessays 1987"
  14. "Collagen synthesis by human skin fibroblasts in culture studies of fibroblasts explanted from papillary and reticular dermis. J Invest Dermatol 1991", pp.410~412
  15. "Fibroblast heterogeneity in the periodontium and other tissues", 32, pp.159~165 (1997)
  16. "Hepatocyte growth factor", scatter factor, a cytokine playing multiple and converse roles, 1997, pp.12, 537~555
  17. "Human KGF is FGF-related with properties of a paracrine effector of epithelial cell growth. Science 1989", pp.752~755, Finch PW Aaronson SA
  18. "Keratinocyte growth factor mRNA expression in periodontal ligament fibroblasts", 105, pp.593~598 (1997)
  19. "Up-regulation of inducible nitric oxide synthase and nitric oxide in Helicobacter pylori-infected human gastric epithelial cells:possible role of interferon-γin polarized nitric oxide secretion", 7, pp.116~128 (2002)
  20. "p38 MAP kinase activation by Clostridium difficile toxin A mediates monocyte necrosis, IL-8 production, and enteritis", pp.1147~1156 (2000)
  21. "The chlorophyllin-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells is associated with ERK deactivation and Cyclin D1 depletion", 16, pp.735~740 (2005)
  22. "Immortalization of normal human oral keratinocyte with type 16 human papillomavirus. Cariconogensis 1991", pp.1627~1631
  23. "Transformation of murine melanocytes by basic fibroblast growth factor cDNA and oncogenes and selective suppression of the transformed phenotype in a reconstituted cutaneous environment. J Cell Biol 1989", pp.3115~3128
  24. "In vivo-like drug responses of human tumors growing in threedimensional", pp.5029~5033
  25. "Organotypic keratinocyte coculture I defined medium with regular epidermal morhogenesis and differentiation", 112, pp.681~691 (1999)
  26. "Epidermal organization and differentiation of HaCaT keratinocytes in organotypic coculture with human dermal fibroblasts", 112, pp.343~353 (1999)
  27. "Induction of PCNA in differentiated kerationcytes of human papilloma virus infected lesions", pp.343~348
  28. "Differentiation dependent up regulation of the human papilloma virus E7 gene reactivates cellular DNA replication in suprabasilar differentiated keratinocytes. Genes Dev 1995", 9, pp.2335~2349 (1995)
  29. "of human skin in organ culture: role for Insulin-like growth factor-1 receptor and EGFR", 291, pp.643~651 (1999)
  30. "A new skin equivalent model: dermal substrate that combines de-epidermized dermis with fibroblasts-populated collagen matrix", 23, pp.132~137 (2000)
  31. "Human oral buccal mucosa reconstructed on dermal substrates: a model for oral epithelial differentiation", 289, pp.677~685 (1997)
  32. "Expression of keratin in normal, immortalized and malignant oral epithelia in organotypic culture", 37, pp.419~430 (2001)
  33. "Reconstituted human oral and esophageal mucosa", 34, pp.46~52 (1998)
  34. "Gene expression of the differentiation-specific keratins (4", 13, and 1, pp.1998, 78[DOI]
  35. "Keratin 14 and 19 expression in normal, dysplastic and malignant oral epithelia. a study using in situ hybridization and immunohistochemistry", 25, pp.293~301 (1996)
  36. "Chracterization of keratin and cell cycle protein expression in cell lines from squamous intraepithelial lesions progressing towards a malignant phenotype", 77, pp.766~775 (1998)

관련 논문AI추천

이 간행물의 다른 논문 (11건)