본문 바로가기
KERD
논문KCI 등재

각화낭치성종양의 이장상피에서 Bcl-2 발현양상과 임상지표

Expression of Bcl-2 in the epithelial lining and clinical findings of keratocystic odotogenic tumor

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류치의학

초록

Purpose: The odontogenic keratocysts demonstrated a high recurrence rate and a biologically aggressive nature. This might be due to unknown factors inherent in the epithelium or enzymatic activity in the fibrous wall. Bcl-2 protein is characterized by its ability to inhibit apoptosis. The aim of this study was to evaluate the expression and distribution of bcl-2 in the OKCs, its possible relationship with the tumorous characteristics, such as the aggressive nature and high recurrence rate, and its usefulness to differentiate OKCs from dentigerous cysts. Materials and Methods: Formalin fixed paraffin-embedded tissue sections of 53 OKCs, and 44 dentigerous cyst were immunohistochemically analyzed quantitatively for the immunoreactivity of the bcl-2 protein with i-solution. Results: More Bcl-2 expression was observed in the OKCs (mean34.387%) than dentigerous cyst (mean11.144%) with statistical significance (P<0.001). Seventeen and 15 of the 32 OKCs in this study showed positivity in the basal layer and basal/suprabasal layers, respectively. In dentigerous cyst, 2 of 3 showed positivity in the basal cell layer. Conclusion: Considering that bcl-2 over expression may lead to the increased survival of epithelial cells, this study demonstrated a possible relationship between the aggressive nature of OKC and the intrinsic growth potential of its lining epithelium. Furthermore, the basal/suprabasal distribution of bcl-2 positive cells was observed in some OKCs, which might have a significant impact on the behavior of cysts. The bcl-2 expression of OKCs can be useful for differentiating OKCs from dentigerous cysts.

Abstract

Purpose: The odontogenic keratocysts demonstrated a high recurrence rate and a biologically aggressive nature. This might be due to unknown factors inherent in the epithelium or enzymatic activity in the fibrous wall. Bcl-2 protein is characterized by its ability to inhibit apoptosis. The aim of this study was to evaluate the expression and distribution of bcl-2 in the OKCs, its possible relationship with the tumorous characteristics, such as the aggressive nature and high recurrence rate, and its usefulness to differentiate OKCs from dentigerous cysts. Materials and Methods: Formalin fixed paraffin-embedded tissue sections of 53 OKCs, and 44 dentigerous cyst were immunohistochemically analyzed quantitatively for the immunoreactivity of the bcl-2 protein with i-solution. Results: More Bcl-2 expression was observed in the OKCs (mean34.387%) than dentigerous cyst (mean11.144%) with statistical significance (P<0.001). Seventeen and 15 of the 32 OKCs in this study showed positivity in the basal layer and basal/suprabasal layers, respectively. In dentigerous cyst, 2 of 3 showed positivity in the basal cell layer. Conclusion: Considering that bcl-2 over expression may lead to the increased survival of epithelial cells, this study demonstrated a possible relationship between the aggressive nature of OKC and the intrinsic growth potential of its lining epithelium. Furthermore, the basal/suprabasal distribution of bcl-2 positive cells was observed in some OKCs, which might have a significant impact on the behavior of cysts. The bcl-2 expression of OKCs can be useful for differentiating OKCs from dentigerous cysts.

참고문헌 (51)

  1. Philipsen HP, "Om Keratocyster(Kolesteatomer) I Kaeberne", Tandlaegebladet, 60, pp.963~980 (1956)
  2. Pindborg JJ, "Studies on odontogenic cyst epithelium: 2.Clinical and roentgenologic aspects of odontogenic keratocysts", Acta Pathol Microbiol Scand, 58, pp.283~294 (1963)
  3. Giuliani M, "Conservative management of a large odontogenic keratocyst: report of a case and review of the literature", J Oral Maxillofac Surg, 64, pp.308~316 (2006)
  4. Barnes L, "Surgical pathology of the head and neck. 1st ed" (1985), Marcel Dekker
  5. el-Hajj G, "Odontogenic keratocysts-a retrospective clinical and histologic study", Int J Oral Maxillofac Surg, 25, pp.124~129 (1996)
  6. Chow HT, "Odontogenic keratocyst: a clinical experience in Singapore", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 86, pp.573~577 (1998)
  7. 천상득, "치성각화낭의 방사선학적 연구", Imaging Science in Dentistry, 35(1), pp.51~54 (2005), 대한구강악안면방사선학회
  8. Shear M, "The aggressive nature of the odontogenic keratocyst: Is it a benign cystic neoplasm? Part 2. Proliferation and genetic studies", Oral Oncol, 38, pp.323~331 (2002)
  9. Madras J, "Keratocystic Odontogenic Tumour: reclassification of the odontogenic keratocyst from cyst to tumour", J Can Dent Assoc, 74, pp.165~165h (2008)
  10. Stoll C, "Cytokeratin expression patterns for distinction of odontogenic keratocysts from dentigerous and radicular cysts", J Oral pathol Med, 34, pp.558~564 (2005)
  11. Meara JG, "Cytokeratin expression in the odontogenic keratocyst", J Oral Maxillofac Surg, 58, pp.862~865 (2000)
  12. August M, "Differentiation of odontogenic keratocysts from nonkeratinizing cyst by use of fineneedle aspiration biopsy and cytokeratin-10 staining", J Oral Maxilloc Surg, 58, pp.935~940 (2000)
  13. 송만용, "낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구", Imaging Science in Dentistry, 34(2), pp.75~79 (2004), 대한구강악안면방사선학회
  14. 이상구, "치성 각화낭종의 면역조직화학적 연구", 대한악안면성형재건외과학회지, 26(5), pp.469~476 (2004), 대한악안면성형재건외과학회
  15. Slootweg PJ, "P53 protein and Ki-67 reactivity in epithelial odontogenic lesions. An immunohistochemical study", J Oral Pathol Med, 24(9), pp.393~397 (1995)
  16. Hockenbery DM, "BCL2 protein is topographically restricted in tissues characterized by apoptotic cell death", Proc Natl Acad Sci USA, 88(16), pp.6961~6965 (1991)
  17. Gee JM, "Immunocytochemical localization of BCL-2 protein in human breast cancers and its relationship to a series of prognostic markers and response to endocrine therapy", Int J Cancer, 59, pp.619~628 (1994)
  18. Colombel M, "Detection of the apoptosis-suppressing oncoprotein bcl-2 in hormone-refractory human prostate cancers", Am J Pathol, 143, pp.390~340 (1993)
  19. Pezzella F, "BCL-2 protein in nonsmall- cell lung carcinoma", N Engle J Med, 329, pp.690~694 (1993)
  20. Skopelitou A, "Topographical immunohistochemical expression of bcl-2 protein in human liver lesions", Anticancer Res, 16, pp.975~978 (1996)
  21. Lauwers GY, "Immunohistochemical evidence of aberrant bcl-2 protein expression in gastric epithelial dysplasia", Cancer, 73, pp.2900~2904 (1994)
  22. Virkaja¨rvi N, "Apoptotic index and apoptosis influencing proteins bcl-2, mcl-1, bax and caspases 3, 6 and 8 in pancreatic carcinoma", Histopathology, 33, pp.432~439 (1998)
  23. Eid H, "Expression of bcl-2 in testicular carcinoma: correlation with tumor progression and MDR1", Pgp, 1998, pp.83, 331~336
  24. Lu QL, "Bcl-2: role in epithelial differentiation and oncogenesis", Hum Pathol, 27, pp.102~110 (1996)
  25. Piattelli A, "Differentiation of odontogenic keratocysts from other odontogenic cysts by the expression of bcl-2 immunoreactivity", Oral Oncol, 34, pp.404~407 (1998)
  26. Toller P, "Origin and growth of cysts of the jaws", Ann R Coll Surg Engl, 40, pp.306~336 (1967)
  27. Gonza′lez-Alva P, "Keratocystic odontogenic tumor: a retrospective study of 183 cases", J Oral Sci, 50, pp.205~212 (2008)
  28. Ogden GR, "P53 protein in odontogenic cysts: increased expression in some odontogenic keratocysts", J Clin Pathol, 45, pp.1007~1010 (1992)
  29. Ahlfors E, "The odontogenic keratocyst: a benign cystic tumor?", J Oral Maxillofac Surg, 42, pp.10~19 (1984)
  30. Brannon RB, "The odontogenic keratocyst; a clinicopathologic study of 312 cases. Part Ⅰ. Clinical features", Oral Surg Oral Med Oral Pathol, 42, pp.54~72 (1976)
  31. Browne RM, "The odontogenic keratocyst: clinical aspects", Br Dent J, 128, pp.225~231 (1970)
  32. Browne RM, "The odontogenic keratocyst: histological features and their correlation with clinical behaviour", Br Dent J, 131, pp.249~259 (1971)
  33. Magnusson BC, "Odontogenic keratocysts: a clicical and histological study with special reference to enzyme histochemistry", J Oral Pathol, 7, pp.8~18 (1978)
  34. Forssell K, "A clinical and radiographic study of odontogenic keratocysts in jaws", Proc Finn Dent Soc, 통권 : 70, pp.121~134 (1974)
  35. Shear M, "Developmental odontogenic cysts: an update", J Oral Pathol Med, 23, pp.1~11 (1994)
  36. Arafat A, "Odontogenic keratocysts", J Baltimore Coll Dental Surg, 36, pp.5~13 (1984)
  37. Philipsen HP, "Keratocystic odontogenic tumour. In World Health Organization classification of tumours: pathology and genetics of head and neck tumours", pp.306~307 (2005), IARC Press
  38. Marker P, "Treatment of large odontogenic keratocysts by decompression and later cystectomy: a long-term follow-up and a histologic study of 23cases", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 82, pp.122~131 (1996)
  39. Donoff RB, "Keratocysts of the jaws", J Oral Surg, 30, pp.800~804 (1972)
  40. Omura S, "Odontogenic keratocyst appearing as a soap-bubble or honeycomb radiolucency: report of a case", J Oral Maxillofac Surg, 55, pp.185~189
  41. Kumar V, "Robbinson and Cotran pathologic basis of disease. 7th ed", pp.26~32 (2005), Elsevier Saunders
  42. Perumalsamy LR, "Notch-activated signaling cascade interacts with mitochondrial remodeling proteins to regulate cell survival", Proc Natl Acad Sci USA, 통권 : 107, pp.6882~6887 (2010)
  43. Kernohan NM, "Regulation of apoptosis by bcl-2 and its related proteins: immunochemical challenges and therapeutic implications", J Pathol, 179, pp.1~3 (1996)
  44. Nicholson DW, "Caspases: killer proteases", Trends Biochem Sci, 22, pp.299~306 (1997)
  45. Villa P, "Caspases and caspase inhibitors", Trends Biochem Sci, 22, pp.388~393 (1997)
  46. Singh BB, "Immunohistochemical evaluation of bcl-2 oncoprotein in oral dysplasia and carcinoma", Oral Surg Oral Med Oral Pathol Oral Oadiol Endod, 85, pp.692~698 (1998)
  47. Lo Muzio L, "Expression of cell cycle and apoptosis-related proteins in sporadic odontogenic keratocysts and odontogenic keratocysts associated with the nevoid basal cell carcinoma syndrome", J Dent Res, 78, pp.1345~1353 (1999)
  48. Jahanshahi GH, "Expression of bcl-2 in the epithelial lining of odontogenic keratocysts", J Dent Tehran Univ Medical Sci, 3(1) (2006)
  49. Singh B, "mmunohistochemical status of bcl-2 oncoprotein in the odontogenic keratocyst. In: Abstract and clinical pathologic conference cases presented at the 52nd Academy of Oral and Maxillofacial Pathology", Oral Surg Oral Med Oral Pathol, 통권 : 86, pp.207~219 (1998)
  50. Birchall MA, "Apoptosis in normal epithelium, premalignant and malignant lesions of the oropharynx and oral cavity: a preliminary study", Eur J Cancer B Oral Oncol, 31B(6), pp.380~383 (1995)
  51. Rodriguez-Villanueva J, "The expression and localization of bcl-2 protein in normal skin and in non-melanoma skin cancers", Pathol Res Pract, 191, pp.391~398 (1995)

관련 논문AI추천

Expansile keratocystic odontogenic tumor in the maxilla: immunohistochemical studies and review of literature강영훈, 박봉욱, 변준호, 최문정대한구강악안면외과학회 · 대한구강악안면외과학회지2013 · 제39권 제4호Expression of cytokeratin 10, 16 and 17 as biomarkers differentiating odontogenic keratocysts from dentigerous cysts최소영, Chin-Soo Kim, Jung-Min Kim대한구강악안면외과학회 · 대한구강악안면외과학회지2012 · 제38권 제2호치성 각화 낭종의 술후 재발양상과 추적관찰 기간의 제안남웅, 강상훈, 김기호, 김남균, 김형준, 박세현, 박형식, 차인호대한구강악안면외과학회 · 대한구강악안면외과학회지2008 · 제34권 제4호치성 각화낭종의 임상적 연구김용덕, 김욱규, 김종렬, 성화식, 신상훈, 이주민, 정인교, 황대석대한구강악안면외과학회 · 대한구강악안면외과학회지2009 · 제35권 제2호Current Concepts and Occurrence of Epithelial Odontogenic Tumors: II. Calcifying Epithelial Odontogenic Tumor Versus Ghost Cell Odontogenic Tumors Derived from Calcifying Odontogenic Cyst김연숙, 이석근대한병리학회 · Journal of Pathology and Translational Medicine2014 · 제48권 제3호Clinical study of keratocystic odontogenic tumorsKazuto Kurohara, Narikazu Uzawa, Nobuyoshi Tomomatsu, Norihiko Okada, Teruo Amagasa, Yasuyuki Michi대한구강악안면외과학회 · 대한구강악안면외과학회지2012 · 제38권 제1호

이 간행물의 다른 논문 (11건)