Rabbit submandibular salivary gland replantation
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초록
Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy.Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, 99mTc scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation.Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight varia-tions from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini. Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.
Abstract
Objectives: To test the feasibility of submandibular salivary gland (SMG) replantation techniques and the survival of the replanted glands. Such a study can provide a rationale for later allotransplantation procedures, along with implementation of conventional and advanced immunosuppression therapy.Materials and Methods: Six SMG replantations were performed in New Zealand white rabbits. One week postoperatively, 99mTc scintigraphy was performed and the uptake ratio and salivary excretion fraction were calculated. Two to four weeks later, submandibular glands were excised, fixed, and stained with H&E for histomorphometric evaluation.Results: Intraoperatively, all glands showed patent blood perfusion except gland 5. Positive tracer uptake and saliva excretion were documented by scintigraphy. On excision, all of the glands except glands 4 and 5 looked viable, with a red color and patent pedicles. Gland 4 was infected and filled with creamy pus, while gland 5 looked pale and necrotic. Histologically, glands 1, 2, 3, and 6 had preserved normal glandular tissue with slight varia-tions from the contralateral normal glands, as their parenchyma was composed of mildly atrophic acini. Conclusion: Four out of six replanted SMGs successfully survived. The glands maintained good viability and function. Such success depends on safe harvesting, short anastomosis time, and strict control of infection.
참고문헌 (13)
- Hanley O, "Reduction of xerostomia in head and neck cancer patients. A critical review of the literature", Radiography, 22(Suppl 1), pp.S57~S63 (2016)
- Mercadante V, "Interventions for the management of radiotherapy-induced xerostomia and hyposalivation: a systematic review and meta-analysis", Oral Oncol, 66, pp.64~74 (2017)
- Kumar PA, "Microvascular submandibular gland transfer for the management of xerophthalmia; an experimental study", Br J Plast Surg, 43, pp.431~436 (1990)
- Spiegel JH, "Microvascular transplantation and replantation of the rabbit submandibular gland", Arch Otolaryngol Head Neck Surg, 127, pp.991~996 (2001)
- Hakim SG, "Radioprotective effect of lidocaine on function and ultrastructure of salivary glands receiving fractionated radiation", Int J Radiat Oncol Biol Phys, 82, pp.e623~e630 (2012)
- Liu XJ, "Carbachol improves the secretion of transplanted submandibular glands during the latent period after microvascular autologous transplantation for severe keratoconjunctivitis sicca", Int J Oral Maxillofac Surg, 45, pp.1273~1279 (2016)
- Su WF, "Microvascular transplantation and replantation of the dog submandibular gland", Eur Arch Otorhinolaryngol, 263, pp.490~494 (2006)
- Lee JS, "Variations in the anatomy of the rabbit cervical carotid artery", Stroke, 25, pp.501~503 (1994)
- Sieg P, "Microvascular submandibular gland transfer for severe cases of keratoconjunctivitis sicca", Plast Reconstr Surg, 106, pp.554~560 (2000)
- Bohuslavizki KH, "Radioprotection of salivary glands by amifostine in high-dose radioiodine therapy", J Nucl Med, 39, pp.1237~1242 (1998)
- Bohuslavizki KH, "Radioprotection of salivary glands by S-2-(3-aminopropylamino)-ethylphosphorothioic (amifostine) obtained in a rabbit animal model", Int J Radiat Oncol Biol Phys, 45, pp.181~186 (1999)
- Hakim SG, "The superficial mandibular gland of the rabbit: a new experimental model for scintigraphic evaluation of salivary glands", Int J Oral Maxillofac Surg, 31, pp.303~308 (2002)
- Geerling G, "Transplantation of the major salivary glands", Dev Ophthalmol, 41, pp.255~268 (2008)