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Random Amplified Polymorphic DNA 분석을통한 Methicillin 내성 황색포도구균의분자역학적 조사

Molecular Epidemiological Typing of Methicillin-Resistant Staphylococcus aureusUsing Random Amplified Polymorphic DNA Analysis

김정만|우종수|전희재
대한감염학회 (The Korean Society Of Infectious Diseases)|2005.02|Infection and Chemotherapy|제37권 제1호|pp. 39-46

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참고문헌

주제분류내과학

초록

목 적 : 본 연구는 중환자실 환자 및 의료종사자에서 검출된 MRSA의 역학조사에 신속하고 간편한 방법인 random amplified polymorphic DNA (RAPD)의 유용성을 평가하고자 하였다.재료 및 방법 : 1998년 10월부터 12월 및 2001년 5월부터 7월까지 동아대학교병원 중환자실 입원 환자 및 의료종사자의 검체 각각 10, 15예 및 8, 5예를 대상으로 mecA gene의 유무와 항균제 감수성 검사를 실시하고, 3가지 primer를 이용하여 RAPD를 시행하였다.결 과 : 환자에서 분리된 MRSA 25주 중 21주(84%)와 의료진에서 분리된 13주 중 12주(92%)가 mecA 양성이었고, mecA 양성 MRSA를 RAPD로 분석한 결과 모두 18가지 유형으로 분류되었다. 결 론:RAPD 방법을 이용한 유전자 형별 분석은 MRSA의 균주 분별에 유용하며, 또한 중환자실에서 유행발생한 MRSA의 신속한 역학조사에 유용할 것으로 생각한다.

Abstract

Background:The random amplified polymorphic DNA (RAPD) analysis was investigated to see if this method could be a useful tool for monitoring of epidemic outbreaks of methicillin-resistant Staphylococcus aureus (MRSA) among patients and healthcare workers (HCW) in the intensive care units (ICU). Methods:Thirty-eight MRSA strains were isolated from patients and HCW in Dong-A University Hospital ICU from October, 1998 to December, 1998 (10 patients and 8 HCW) and May, 2001 to July, 2001 (15 patients and 5 HCW). All strains were typed according to antimicrobial susceptibility and RAPD analysis patterns. mecA genes were detected using polymerase chain reaction (PCR). Results:Twenty one of 25 (84%) and 12 of 13 (92%) MRSA, isolated from patients and HCW, respectively, were mecA positive. mecA positive MRSA were classified into 18 different types by RAPD analysis. Conclusion:DNA fingerprinting using RAPD analysis is a simple, effective, and rapid method for discriminating MRSA strains, and may be applicable in detecting outbreaks of S. aureus infections in the ICU.

참고문헌 (46)

  1. "Methicillin-resistant staphylococci", pp.650~3
  2. "The dominance of methicillin-resistant staphylococci in a county hospital", 15, pp.161~5 (1968)
  3. "The natural resistance of staphylococci to methicillin and oxacillin", 10, pp.495~506 (1965)
  4. "The emergence of methicillin-resistant Staphylococcus aureus infections in United States hospitals.Possible role of the house staff-patient transfer circuit", 97, pp.297~308 (1982)
  5. "Methicillin-resistant Staphylococcus aureus:risk factors for infection-colonization and clonal heterogeneity in intensive care units", 33, pp.497~500 (1998)
  6. "Outbreaks of infection with methicillin-resistant Staphylococcus aureus on neonatal and burn units of a new hospital", 105, pp.215~28 (1990)
  7. "Value of molecular epidemiologic analysis in a nosocomial methicillin-resistant Staphylococcus aureus outbreak", 270, pp.1323~8 (1993)
  8. "Environmental study of a methicillin-resistant Staphylococcus aureus epidemic in a burn unit", 18, pp.683~8 (1983)
  9. "Methicillin-Resistant Staphylococcus aureus. In : Mayhall CG eds. Hospital Epidemiology and Infection Control,2nd ed. Lippincott Williams & Wilkins", pp.347~64 (1999)
  10. "An outbreak of infections caused by strains of Staphylococcus aureus resistant to methicillin and aminoglycosides", pp.280~7 (1979)
  11. "Methicillin-resistant Staphylococcus aureus:interstate spread of nosocomial infections with emergence of gentamicin-methicillin resistant strains", 1, pp.81~9 (1980)
  12. "Methicillin-resistant Staphylococcus aureus:a consensus review of the microbiology,pathogenesis,and epidemiology with implications for prevention and management", 94, pp.313~28 (1993)
  13. "Comparison of epidemiological markers used in the investigation of an outbreak of methicillin-resistant Staphylococcus aureus infections", 18, pp.395~9 (1983)
  14. "Plasmid analysis in the study of the epidemiology of nosocomial gram-positive cocci", 8, pp.705~12 (1986)
  15. "Epidemiologic study of methicillin-resistant Staphylococcus aureus using plasmid analysis", 12, pp.347~54 (1992)
  16. "Molecular typing of S.aureus on the basis of coagulase gene polymorphisms", 30, pp.1642~5 (1992)
  17. "Coagulase types of Staphylococcus aureus and its relationship with antibiotics susceptibility pattern", 16, pp.928~34 (1996)
  18. "Molecular typing of the methicillin-resistant determinant(mec)and coagulase typing as epidemiologic markers for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus", 17, pp.588~97 (1997)
  19. "DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates", 30, pp.967~73 (1992)
  20. "Typing of consecutive methicillin-resistant Staphylococcus aureus isolates from intensive care unit patients and staff with pulsed-field gel electrophoresis", 10, pp.309~12 (1998)
  21. "Pulsed- field gel electrophoresis is a useful tool in the monitoring of methicillin-resistant Staphylococcus aureus epidemic outbreaks in the intensive care unit", 27, pp.447~51 (1999)
  22. "Validation of Binary typing for Staphylococcus aureus strains", 37, pp.664~74 (1999)
  23. "Molecular epidemiological typing of clinical strains of methicillin-resistant Staphylococcus aureus", 2, pp.40~8 (1999)
  24. "Analysis of Staphylococcus aureus by using a randomly amplified polymorphic DNA assay", 35, pp.3092~7 (1997)
  25. "Manual of clinical microbiology", (ver fc), pp.264~82 (1999), ASM Press
  26. "Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction", 29, pp.2240~4 (1991)
  27. "National Committee for Clinical Laboratory Stan- dard Performance standards for antimicrobial disk susceptibility tests-sixth edition National Committee for Clinical Laboratory Standard pM2-A6" (1998)
  28. "Evaluating two methods for fingerprinting genomes of Actinobacillus actinomycetemcomitans", 8, pp.337~43 (1993)
  29. "Intensive care unit design and environmental factors in the acquisition of infection", 45, pp.255~62 (2000)
  30. "Reduced susceptibility of Staphylococcus aureus to vancomycin-Japan", pp.624~6
  31. "Vancomycin resistant Staphylococcus aureus in Korea", 2, pp.55~ (1999)
  32. "Prevalence of teicoplanin-resistant Staphylococcus aureus and evaluation of the disk diffusion test for testing the susceptibility of Staphylococci to teicoplanin", 30, pp.151~5 (1988)
  33. "Antimicrobial susceptibilities of glycopeptides,arbekacin and quinupristin", dalfopristin against Staphylococcus aureus isolates, 2001, pp.33, 261~6
  34. "Fingerprinting genomes using PCR with arbitary primers", 18, pp.7213~8 (1990)
  35. "Multicenter evaluation of arbitrarily primed PCR for typing of Staphylococcus aureus strains", 33, pp.1537~47 (1995)
  36. "Comparison of phage typing and DNA fingerprinting by PCR for discrimination of methicillin-resistant Staphylococcus aureus isolates", 31, pp.798~803 (1993)
  37. "Molecular epidemiologic analysis of Staphylococcus aureus isolated from clinical specimens", 12, pp.190~8 (1997)
  38. "Evaluation of an antibiogram-resistogram typing scheme for methicillin-resistant Staphlyococcus aureus", 41, pp.441~7 (1994)
  39. "MRSA in the critically ill", 48, pp.98~102 (2001)
  40. "High frequency of colonization and absence of identifiable risk factors for methicillin-resistant Staphylococcus aureus(MRSA)in intensive care units in Brazil", 5, pp.1~7 (2001)
  41. "Cost analysis concerning MRSA-infection in ICU", 34, pp.409~13 (1999)
  42. "Methicillin-resistant staphylococcal coloniztion and infection in a long-term care facility", 114, pp.107~12 (1991)
  43. "Spread of methicillin-resistant Staphylococcus aureus in a hospital after exposure to a health care worker with chronic sinusitis", 17, pp.496~504 (1993)
  44. "Treatment of infection and colonization caused by methicillin-resistant Staphylococcus aureus", 12, pp.29~35 (1991)
  45. "Elimination of coincident Staphylococcus aureus nasal and hand carriage with intranasal application of mupirocin calcium ointment", pp.101~6 (1991)
  46. "Methicillin-resistant Staphylococcus aureus outbreak:a consensus panel's definition and management guidelines", 26, pp.102~10 (1998)

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Multilocus Sequence Typing of Clonal Changes of Methicillin-resistant Staphylococcus aureus Isolated from Intensive Care Unit Patients: 1996 versus 2004김의종, 이종윤, 허지영, 홍기호대한임상미생물학회 · Annals of Clinical Microbiology2006 · 제9권 제2호충남지역 종합병원 중환자실 환경과 환자로부터 분리한 메티실린 내성 황색포도알균(MRSA)의 분자유전학적 특성김상하, 김성현, 김영권, 김혜숙, 박성배, 현성희대한임상검사과학회 · 대한임상검사과학회지2018 · 제50권 제2호AP-PCR을 이용한 다제내성 Staphylococcus aureus의 유전형 분석손승렬, 신경현, 홍승복대한임상검사과학회 · 대한임상검사과학회지2004 · 제36권 제2호메티실린 내성 황색포도알균(Methicillin Resistant Staphylococcus aureus, MRSA)의 분자역학 연구에서 IRS-PCR (Infrequent- Restriction-Site PCR)의 응용권재철, 김시현, 박선희, 박철민, 신나영, 유진홍, 이동건, 최수미, 최정현대한감염학회 · Infection and Chemotherapy2011 · 제43권 제5호한국에서 분리된 methicillin 내성 포도상구균(MRSA)에서 multoplex PCR에 의한 nuc, mecA, enterotoxon 유전자들과 tst 유전자의 분포에 관한 연구윤효숙, 이승관, 이창규, 황선철대한임상검사과학회 · 대한임상검사과학회지1997 · 제29권 제1호환자의 비강으로부터 분리된 메티실린 내성 황색 포도알균의 분자 아형 분석임재원, Heechul Park, Jiyoung Lee, Jungho Kim, Jun Seong Kim, Sang-Ha Kim, Sung-Bae Park, Sunghyun Kim, Young Kwon Kim대한임상검사과학회 · 대한임상검사과학회지2020 · 제52권 제2호

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