CTX-M형 ESBL 생성 비장티푸스성 살모넬라의 특성
Characteristics of CTX-M Type Extended Spectrum ß-lactamase Producing Non-typhoidal Salmonella Isolates
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Extended-spectrum ß-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital. Materials and Methods: Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing Results: Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime. Conclusions: ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M.
Abstract
Background: Extended-spectrum ß-lactamase (ESBL)-producing Salmonella have been increasingly reported worldwide. ESBL-producing Salmonella is of particular concern since children cannot be treated with quinolones. This study was conducted to determine the phenotypic and genetic characteristics of ESBL-producing Salmonella in a tertiary hospital. Materials and Methods: Four clinical ESBL-producing isolates of non-typhoidal Salmonella were collected during 2001 to 2009. Antimicrobial susceptibility was determined by disk diffusion test and VITEK-II system. ESBL production was tested by ESBL phenotypic confirmatory test. TEM, SHV, CTX-M1, CTX-M2, CTX-M8, and CTX-M9 type ESBL genes were detected by PCR amplification, and PCR products were subjected to direct sequencing Results: Phenotypic confirmatory test showed that 4 of the 300 non-typhoidal Salmonella isolates were ESBL-producing: 3 S. Enteritidis and 1 S. Typhimurium. All 4 isolates were recovered during the past 1 year period. All 3 S. Enteritidis harbored CTX-M-15, while the S. Typhimurium harbored CTX-M-14. All CTX-M-15-producing S. Enteritidis isolates showed resistance both to cefotaxime and ceftazidime, while the CTX-M-14-producing S. Enteritidis were resistant only to cefotaxime. Conclusions: ESBL-producing nontyphoidal Salmonella has emerged recently and the type of ESBL has switched from TEM and SHV to CTX-M.
참고문헌 (17)
- Hohmann EL, "Nontyphoidal salmonellosis", Clin Infect Dis, 32, pp.263~269 (2001)
- Lee HJ, "Salmonellosis", Korean Journal of Clinical Microbiology, 4, pp.5~10 (2001)
- Seo S, "The serogroup and antimicrobial resistance of Salmonella spp. isolated from the clinical specimens during 6 years in a tertiary university hospital", Korean Journal of Clinical Microbiology, 7, pp.72~76 (2004)
- Biedenbach DJ, "Analysis of Salmonella spp . with resistance to extended-spectrum cephalosporins and f luoroquinolones isolated in North America and Latin America: report from the SENTRY antimicrobial surveillance program (1997-2004)", Diagn Microbiol Infect Dis, 54, pp.13~21 (2006)
- Lee K, "Diversity of TEM-52 extended-spectrum beta-lactamase-producing non-typhoidal Salmonella isolates in Korea", Journal of Antimicrobial Chemotherapy, 52, pp.493~496 (2003)
- Yong D, "Nosocomial outbreak of pediatric gastroenteritis caused by CTX-M-14-type extended-spectrum beta?lactamase- producing strains of Salmonella enterica serovar London", Journal of Clinical Microbiology, 43, pp.3519~3521 (2005)
- Leibovitz E, "The use of fluoroquinolones in children", Curr Opin Pediatr, 18, pp.64~70 (2006)
- Performance standards for antimicrobial susceptibility testing, "Clinical and Laboratory Standards Institute", Clinical and Laboratory Standards Institute, ~, pp.CLSI Document M100-S18 (2008)
- Performance standards for antimicrobial susceptibility testing, "Clinical and Laboratory Standards Institute", Clinical and Laboratory Standards Institute, ~, pp.CLSI Document M100-S19 (2009)
- 박용정, "Prevalence of the Extended-Spectrum β-Lactamase and qnr Genes in Clinical Isolates of Escherichia coli", The Korean Journal of Laboratory Medicine, 29(3), pp.218~223 (2009), 대한진단검사의학회
- Arlet G, "Salmonella resistant to extended-spectrum cephalosporins: prevalence and epidemiology", Microbes Infect, 8, pp.1945~1954 (2006)
- Kim JY, "Case report bacteremia due to Salmonella enterica serotype Montevideo producing plasmid-mediated AmpC beta-lactamase (DHA-1)", Ann Clin Lab Sci, 34, pp.214~217 (2004)
- Coque TM, "Increasing prevalence of ESBL-producing Enterobacteriaceae in Europe", Euro Surveill, 13, pp.19051~ (2008)
- Hawkey PM, "Prevalence and clonal it y of extendedspectrum beta-lactamases in Asia", Clin Microbiol Infect, 14(1), pp.159~165 (2008)
- Rotimi VO, "Emergence of CTX-M-15 type extended-spectrum beta-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates", J Med Microbiol, 57, pp.881~886 (2008)
- 오지은, "CTX-M형 Extended-Spectrum -Lactamase 생성 Escherichia coli의 확산 및 CTX-M-12의 출현", The Korean Journal of Laboratory Medicine, 25(4), pp.252~258 (2005), 대한진단검사의학회
- Pai H, "Survey of extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae : prevalence of TEM-52 in Korea", J Clin Microbiol, 37, pp.1758~1763 (1999)