2000년도 제주도의 이질 유행에서 분리된 Shigella sonnei가 보유한 내성 플라스미드의 분석
Characterization of Resistance Plasmid of Shigella sonnei Isolates from the Outbreak at Cheju Island in 2000
2
조회
0
다운로드
0
인용
0
참고문헌
초록
2000년도 5월에서 8월까지 제주도에 Shigella sonnei 장염의 유행이 있었다. 저자들은 유행당시 치료 실패례에서 분리된 이질균 54균주의 내성 플라스미드를 분석하였다. 54균주는 ampicillin, streptomycin, tetracycline, 및 trimethoprim-sulfamethoxazole에 내성이었다. Ampicillin, streptomycin, 및 tetracycline 내성은 약 80 kb 크기의 접합성 플라스미드에 의하여 전이되었으나 trimethoprim-sulfamethoxazole은 플라스미드에 의하여 전파되지 않았다. 플라스미드의 내성 지역을 클로닝하였다. 총 8384 bp 염기서열을 분석한 결과, strA, strB, tetR, 및 sul1 유전자가 존재하였고 기술된 순서대로 위치하였다. 54균주는 같은 크기의 플라스미드를 보유하고 있었으며 같은 리보타이핑 소견을 보여주어 유행내 단일균의 전파를 제시하였다.
Abstract
A large outbreak of Shigella sonnei gastrointestinal infections occurred at Cheju Island in Korea from May to August 2000. We selected 54 strains which were isolated from the primary treatment failure cases in the outbreak, and characterized the resistance-determining region of the R-plasmid. The 54 strains showed same antimicrobial resistance patterns; resistance against ampicillin, streptomycin, tetracycline, and trimethoprim- sulfamethoxazole. The resistance to ampicillin, streptomycin, and tetracycline were mediated by a conjugable plasmid of about 80 kb size, but the trimethoprim- sulfamethoxazole resistance was not transferred by this plasmid. The R-determining region of the plasmid was cloned and characterized. The 8,384 bp sequences contained resistance genes in the following order : strA, strB, tetR, tetA, and sul1. Fifty four isolates harbored the same sized plasmid and showed same ribotyping patterns, which suggested the clonal spread of S. sonnei in the outbreak.
참고문헌 (11)
- "Comparative antibiotic resistance of diarrheal pathogens from Vietnam and Thailand,1996-1999", 8, pp.175~180 (2002)
- "Antimicrobial resistance trends in Shigella serogroups isolated in Israel, 2990-1995.", 19, pp.108~111 (2000)
- "Epidemic strains of Shigella sonnei biotype g carrying integrons", 40, pp.1538~1540 (2002)
- "Typing and characterization of mechanisms of resistance of Shigella spp isolated from feces of children under 5 years of age from Ifakara, Tanzania", 37, pp.3113~3117 (1999)
- "Emergence of antimicrobial resistant shigellosis in Oregon", 30, pp.515~519 (2000)
- "Shigella spp. surveillance in Indonesia: the emergence or reemergence of S. dysenteriae", 7, pp.137~140 (2001)
- "Report of epidemiologic investigation of Cheju outbreak of Shigella sonnei infection" (2000)
- "Identification of CTX-M-14 extended-spectrum beta-lactamase in clinical isolates of Shigella sonnei and Klebsiella pneumoniae in Korea", 39, pp.3747~3749 (2001)
- "Performance standards for antimicrobial disk susceptibility tests; approved standard M2-A7 7th ed." (2000), National Committee for Clinical Laboratory Standards
- "Survey of extended-spectrum β-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae Prevalence of TEM-52 in Korea", 37, pp.1758~1763 (1999)
- "The use of analytical isoelectric focusing for detection and identification of β-lactamases", 88, pp.169~78 (1975)