Genotypic Diversity of Multidrug Resistant Shigella species from Iran
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Background: In many developing countries, shigellosis is endemic and also occurs in epidemics and treatment of multidrug-resistant (MDR) isolates are important. The aims of this study were to determine the antimicrobial susceptibility, prevalence of class 1 and 2 integrons and the clonal relatedness of isolates. Materials and Methods: Antimicrobial susceptibility tests were performed by disc diffusion method. Polymerase chain reaction (PCR)-sequencing technique was employed for detection and characterization of integrons. The genetic relatedness was evaluated by using enterobacterial repetitive intergenic consensus (ERIC) PCR. Results: There was a high percentage of resistance to trimethoprim-sulfamethoxazole (TMP/SMX) (93.7%), ampicillin (AMP) (87.3%), streptomycin (STR) (84.5%) and tetracycline (TET) (78.9%). MDR phenotype was seen in 95.1% of total isolates. Most common MDR profile was TMP/SMX/STR/AMP resistant pattern. Among the 142 Shigella spp. analyzed in this study, 28 isolates were positive for class 1 integron with two types of gene cassette arrays (dfrA17/aadA5 = 31.7% and dfrA7 = 3.8%). The class 2 integron was more frequently detected among the isolates (94.7%) with dfrA1/sat1/aadA1 (69.4%) and dfrA1/sat1 (30.6%) gene cassettes. ERIC-PCR results showed 6, 5, 4 and 3 main genotypes among S. flexneri, S. sonnei, S. boydii and S. dysenteriae isolates, respectively. Conclusion: Our findings revealed that multidrug resistant Shigella species with high prevalence of class 2 integron were very common in Iran. In addition, ERIC-PCR patterns showed limited variety of clones are responsible for shigellosis in the region of the study.
Abstract
Background: In many developing countries, shigellosis is endemic and also occurs in epidemics and treatment of multidrug-resistant (MDR) isolates are important. The aims of this study were to determine the antimicrobial susceptibility, prevalence of class 1 and 2 integrons and the clonal relatedness of isolates. Materials and Methods: Antimicrobial susceptibility tests were performed by disc diffusion method. Polymerase chain reaction (PCR)-sequencing technique was employed for detection and characterization of integrons. The genetic relatedness was evaluated by using enterobacterial repetitive intergenic consensus (ERIC) PCR. Results: There was a high percentage of resistance to trimethoprim-sulfamethoxazole (TMP/SMX) (93.7%), ampicillin (AMP) (87.3%), streptomycin (STR) (84.5%) and tetracycline (TET) (78.9%). MDR phenotype was seen in 95.1% of total isolates. Most common MDR profile was TMP/SMX/STR/AMP resistant pattern. Among the 142 Shigella spp. analyzed in this study, 28 isolates were positive for class 1 integron with two types of gene cassette arrays (dfrA17/aadA5 = 31.7% and dfrA7 = 3.8%). The class 2 integron was more frequently detected among the isolates (94.7%) with dfrA1/sat1/aadA1 (69.4%) and dfrA1/sat1 (30.6%) gene cassettes. ERIC-PCR results showed 6, 5, 4 and 3 main genotypes among S. flexneri, S. sonnei, S. boydii and S. dysenteriae isolates, respectively. Conclusion: Our findings revealed that multidrug resistant Shigella species with high prevalence of class 2 integron were very common in Iran. In addition, ERIC-PCR patterns showed limited variety of clones are responsible for shigellosis in the region of the study.
참고문헌 (30)
- Ahmed AM, "Molecular characterization of multidrug-resistant Shigella spp. of food origin", Int J Food Microbiol, 194, pp.78~82 (2015)[DOI]
- Tajbakhsh M, "Antimicrobial-resistant Shigella infections from Iran: an overlooked problem?", J Antimicrob Chemother, 67, pp.1128~1133 (2012)[DOI]
- Pan JC, "Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri", J Antimicrob Chemother, 58, pp.288~296 (2006)[DOI]
- Ranjbar R, "Antibiotic sensitivity patterns and molecular typing of Shigella sonnei strains using ERIC- PCR", Iran J Public Health, 42, pp.1151~1157 (2013)
- Memish ZA, "Impact of travel on international spread of antimicrobial resistance", Int J Antimicrob Agents, 21, pp.135~142 (2003)[DOI]
- Perilla MJ, "Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world" (2003)
- Guardabassi L, "Distribution and in-vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains", J Med Microbiol, 49, pp.929~936 (2000)[DOI]
- Clinical, "Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Document M100-S25", CLSI, ~ (2015)
- Rezaee MA, "Comparison of the antibiotic resistance patterns among Shigella species isolated from pediatric hospital between 1995-1999 and 2009- 2013 in North-West of Iran", J Anal Res Clin Med, 2, pp.118~122 (2014)
- Ahmed AM, "Molecular characterization of multidrug-resistant avian pathogenic Escherichia coli isolated from septicemic broilers", Int J Med Microbiol, 303, pp.475~483 (2013)[DOI]
- Tamang MD, "Emergence of multidrug-resistant Salmonella enterica serovar Typhi associated with a class 1 integron carrying the dfrA7 gene cassette in Nepal", Int J Antimicrob Agents, 30, pp.330~335 (2007)[DOI]
- Mazel D, "Antibiotic resistance in the ECOR collection: integrons and identification of a novel aad gene", Antimicrob Agents Chemother, 44, pp.1568~1574 (2000)[DOI]
- Dalla-Costa LM, "Characterisation of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR", J Med Microbiol, 47, pp.227~234 (1998)[DOI]
- Khakabimamaghani S, "GelClust: a software tool for gel electrophoresis images analysis and dendrogram generation", Comput Methods Programs Biomed, 111, pp.512~518 (2013)[DOI]
- Zhang J, "Antimicrobial resistance of Shigella spp. from humans in Shanghai, China, 2004-2011", Diagn Microbiol Infect Dis, 78, pp.282~286 (2014)[DOI]
- Eftekhari N, "Genetic diversity of Shigella spp. and their integron content", Foodborne Pathog Dis, 10, pp.237~242 (2013)[DOI]
- Alizadeh-Hesar M, "Clonal dissemination of a single Shigella sonnei strain among Iranian children during Fall 2012 in Tehran, IR Iran", Infect Genet Evol, 34, pp.260~266 (2015)[DOI]
- Mardaneh J, "Prevalence of Shigella species and antimicrobial resistance patterns of isolated strains from infected pediatrics in Tehran", Int J Enteric Pathog, 1, pp.e10705~ (2013)
- Young HK, "Plasmid trimethoprim resistance in Vibrio cholerae: migration of the type I dihydrofolate reductase gene out of the Enterobacteriaceae", J Antimicrob Chemother, 17, pp.697~703 (1986)[DOI]
- Najibi S, "Distribution of class 1 integrons among enteropathogenic Escherichia coli", Can J Microbiol, 58, pp.637~643 (2012)[DOI]
- Cui X, "Antimicrobial resistance of Shigella flexneri serotype 1b isolates in China", PLoS One, 10, pp.e0129009~ (2015)[DOI]
- Tariq A, "Molecular profiling of antimicrobial resistance and integron association of multidrug-resistant clinical isolates of Shigella species from Faisalabad, Pakistan", Can J Microbiol, 58, pp.1047~1054 (2012)[DOI]
- McIver CJ, "Epidemic strains of Shigella sonnei biotype g carrying integrons", J Clin Microbiol, 40, pp.1538~1540 (2002)[DOI]
- Jove T, "Inverse correlation between promoter strength and excision activity in class 1 integrons", PLoS Genet, 6, pp.e1000793~ (2010)[DOI]
- Paulsen IT, "The 3'conserved segment of integrons contains a gene associated with multidrug resistance to antiseptics and disinfectants", Antimicrob Agents Chemother, 37, pp.761~768 (1993)[DOI]
- Peirano G, "Occurrence of integrons and resistance genes among sulphonamide-resistant Shigella spp. from Brazil", J Antimicrob Chemother, 55, pp.301~305 (2005)[DOI]
- Young-hee Jin, "Antimicrobial Resistance Patterns and Characterization of Integrons of Shigella sonnei Isolates in Seoul, 1999-2008", The Journal of Microbiology, 48(2), pp.236~242 (2010), 한국미생물학회
- Das A, "The emergence of quinolone resistant Shigella sonnei, Pondicherry, India", PLoS One, 11, pp.e0160290~ (2016)[DOI]
- Terajima J, "A multi-prefectural outbreak of Shigella sonnei infections associated with eating oysters in Japan", Microbiol Immunol, 48, pp.49~52 (2004)[DOI]
- Navia MM, "Typing and characterization of mechanisms of resistance of Shigella spp. isolated from feces of children under 5 years of age from Ifakara, Tanzania", J Clin Microbiol, 37, pp.3113~3117 (1999)