Degradation of Lignocelluloses in Rice Straw by BMC-9, a Composite Microbial System
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초록
To evaluate the potential utility of pretreatment of raw biomass with a complex microbialsystem, we investigated the degradation of rice straw by BMC-9, a lignocellulosedecomposition strain obtained from a biogas slurry compost environment. The degradationcharacteristics and corresponding changes in the bacterial community were assessed. Theresults showed that rapid degradation occurred from day 0 to day 9, with a peak total biomassbacterium concentration of 3.3 × 108 copies/ml on day 1. The pH of the fermentation brothdeclined initially and then increased, and the mass of rice straw decreased steadily. Thehighest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formicacid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanseactivity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. Thegreatest diversity among the microbial community also occurred on day 9, with the presenceof bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our resultsindicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice strawunder relatively inexpensive conditions, and the optimum fermentation time is 9 days.
Abstract
To evaluate the potential utility of pretreatment of raw biomass with a complex microbialsystem, we investigated the degradation of rice straw by BMC-9, a lignocellulosedecomposition strain obtained from a biogas slurry compost environment. The degradationcharacteristics and corresponding changes in the bacterial community were assessed. Theresults showed that rapid degradation occurred from day 0 to day 9, with a peak total biomassbacterium concentration of 3.3 × 108 copies/ml on day 1. The pH of the fermentation brothdeclined initially and then increased, and the mass of rice straw decreased steadily. Thehighest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formicacid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanseactivity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. Thegreatest diversity among the microbial community also occurred on day 9, with the presenceof bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our resultsindicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice strawunder relatively inexpensive conditions, and the optimum fermentation time is 9 days.
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