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Identification of the σ 70-Dependent Promoter Controlling Expression of the ansPAB Operon of the Nitrogen-Fixing Bacterium Rhizobium etli

Angélica Moreno-Enríquez|Zahaed Evangelista-Martínez|Luis Servín-González|María Elena Flores-Carrasco
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2015|Journal of Microbiology and Biotechnology|제25권 제8호|pp. 1241-1245|DOI 10.4014/jmb.1503.03009

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참고문헌

키워드

Asparagine operonσ70 transcription factorRhizobium etliS1-nuclease mapping
주제분류생물학

초록

The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.

Abstract

The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis of this region showed -10 and -35 elements, which were consistent with consensus sequences for R. etli promoters that are recognized by the major form of RNA polymerase containing the σ70 transcription factor. Mutation studies affecting several regions located upstream of the transcriptional start site confirmed the importance of these elements on transcriptional expression.

참고문헌 (26)

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