An Environmentally Friendly and Efficient Method for Extraction of PHB Biopolymer with Non-Halogenated Solvents
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The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25°C until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103°C and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 106 compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
Abstract
The present study developed an efficient and environmentally friendly method for recovering polyhydroxybutyrate (PHB) from Cupriavidus necator. Several non-halogenated solvents were tested and it was found that butyl acetate and ethyl acetate are powerful solvents for the biopolymer. Testing was performed to examine the effects of temperature (25°C until temperature below solvent boiling points) and heating incubation time (0-60 min) on the two solvents. Butyl acetate had a higher recovery level (96%) and product purity (up to 99%) than ethyl acetate at 103°C and a heating incubation time of 30 min. Under these conditions, PHB recorded the highest molecular weight of 1.4 × 106 compared with the standard procedure (i.e., recovery using chloroform). The proposed strategy showed that butyl acetate is a good alternative to halogenated solvents such as chloroform for recovery of PHB.
참고문헌 (28)
- Akita S, "Solution properties of poly(D-β-hydroxybutyrate). 1. Biosynthesis and characterization", Macromolecules, 9, pp.774~780 (1976)
- Anderson-Cook CM, "Response surface design evaluation and comparison", J. Stat. Plan. Inference, 139, pp.629~641 (2009)
- Anis SN, "Increased recovery and improved purity of PHA from recombinant Cupriavidus necator", Bioengineered, 4, pp.115~118 (2013)
- Aramvash A, "Statistical physical and nutrient optimization of bioplastic polyhydroxybutyrate production by Cupriavidus necator", Int. J. Environ. Sci. Technol, 12, pp.2307~2316 (2015)
- Bensch M, "High throughput screening techniques in downstream processing: preparation, characterization, and optimization of aqueous two-phase systems", Chem. Eng. Sci, 62, pp.2011~2021 (2007)
- Chan CH, "Crystallization and melting behavior of poly(3-hydroxybutyrate)-based blends", Macromol. Chem. Phys, 205, pp.664~675 (2004)
- Chen GQ, "The application of polyhydroxyalkanoates as tissue engineering materials", Biomaterials, 26, pp.6565~6578 (2005)
- Chen GQ, "Biodegradable Polymers for Industrial Applications", pp.32~37 (2005), CRC Press
- Daniel WW, "Biostatistics: A Foundation for Analysis in the Health Sciences" (2013), John Wiley & Sons
- Fiorese ML, "Recovery of polyhydroxybutyrate (PHB)from Cupriavidus necator biomass by solvent extraction with 1,2-propylene carbonate", Eng. Life Sci, 9, pp.454~461 (2009)
- Hahn SK, "Optimization of microbial poly (3-hydroxybutyrate) recover using dispersions of sodium hypochlorite solution and chloroform", Biotechnol. Bioeng, 44, pp.256~261 (1994)
- Jung YM, "Metabolic engineering of Alcaligenes eutrophus through the transformation of cloned phbCAB genes for the investigation of the regulatory mechanism of polyhydroxyalkanoate biosynthesis", Enzyme Microb. Technol, 26, pp.201~208 (2000)
- Khanna S, "Statistical media optimization studies for growth and PHB production by Ralstonia eutropha", Process Biochem, 40, pp.2173~2182 (2005)
- Kim BS, "Production of poly (3-hydroxybutyric-co-3-hydroxyvaleric acid) by fed-batch culture of Alcaligenes eutrophus with substrate control using online glucose analyzer", Enzyme Microb. Technol, 16, pp.556~561 (1994)
- Kunasundari B, "Isolation and recovery of microbial polyhydroxyalkanoates", Express Polym. Lett, 5, pp.620~634 (2011)
- Lafferty RM, "Cyclic carbonic acid esters as solvents for poly-(beta)-hydroxybutyric acid", US Patent No. 4101533 A, ~ (1978)
- Liu J, "Optimization of nutritional conditions for nattokinase production by Bacillus natto NLSSE using statistical experimental methods", Process Biochem, 40, pp.2757~2762 (2005)
- Luengo JM, "Bioplastics from microorganisms", Curr. Opin. Microbiol, 6, pp.251~260 (2003)
- Lee SY, "Removal of endotoxin during purification of poly(3-hydroxybutyrate) from gramnegative bacteria", Appl. Environ. Microbiol, 65, pp.2762~2764 (1999)
- Martino L, "Recovery of amorphous polyhydroxybutyrate granules from Cupriavidus necator cells grown on used cooking oil", Int. J. Biol. Macromol, 71, pp.117~123 (2014)
- Montgomery DC, "Design and Analysis of Experiments", pp.392~510 (2001), John Wiley & Sons
- Riedel SL, "Recovery of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutropha cultures with non-halogenated solvents", Biotechnol. Bioeng, 110, pp.461~470 (2013)
- Sakamuri R., "Kirk-Othmer Encyclopedia of Chemical Technology", pp.497~526 (2005), John Wiley & Sons
- Sevastianov VI, "Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood", J. Biomater. Sci. Polym. Ed, 14, pp.1029~1042 (2003)
- Sim SJ, "PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo", Nat. Biotechnol, 15, pp.63~67 (1997)
- Terada M, "Determination of solubility parameters for poly (3-hydroxyalkanoates)", Int. J. Biol. Macromol, 25, pp.207~215 (1999)
- Valappil SP, "Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV", J. Biotechnol, 132, pp.251~258 (2007)
- Wang ZW, "Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp. using response surface methodology", Bioresour. Technol, 99, pp.8245~8251 (2008)