Glucosyl Rubusosides by Dextransucrases Improve the Quality of Taste and Sweetness
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초록
Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59–66% as analyzed by TLC and HPLC. Evaluation of the sweetness of these glucosyl rubusosides showed that their quality of taste, in particular, was superior to that of rubusoside. These results indicate that transglucosylation at the 13-O-glucosyl moiety of rubusoside by different regioselective dextransucrases can be applicable for increasing its sweetness and quality of taste.
Abstract
Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59–66% as analyzed by TLC and HPLC. Evaluation of the sweetness of these glucosyl rubusosides showed that their quality of taste, in particular, was superior to that of rubusoside. These results indicate that transglucosylation at the 13-O-glucosyl moiety of rubusoside by different regioselective dextransucrases can be applicable for increasing its sweetness and quality of taste.
참고문헌 (17)
- Darise M, "Enzymic transglucosylation of rubusoside and the structure-sweetness relationship of steviol-bisglycosides", Agric. Biol. Chem, 48, pp.2483~2488 (1984)
- George Thompson AM, "Inhibition of human GLUT1 and GLUT5 by plant carbohydrate products; insights into transport specificity", Sci. Rep, 5, pp.12804~ (2015)
- Ishikawa H, "Production of stevioside and rubusoside derivatives by transfructosylation of β-fructofuranosidase", Agric. Biol. Chem, 54, pp.3137~3143 (1990)
- Kim YM, "Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001", Microbiol. Res, 165, pp.384~391 (2010)
- Kitahata S, "Production of rubusoside derivatives by transgalactosylation of various α-galactosidases", Agric. Biol. Chem, 53, pp.2929~2934 (1989)
- 고진아, "Large Increase in Leuconostoc citreum KM20 Dextransucrase Activity Achieved by Changing the Strain", Inducer Combination in an E. coli Expression System, 2012, pp.22(4), 510~515[DOI]
- Hyang-Yeol Lee, "Enzymatic Synthesis of 2-Phenoxyethanol Galactoside by Whole Cells of β-Galactosidase-Containing Escherichia coli", Journal of Microbiology and Biotechnology, 24(9), pp.1254~1259 (2014), 한국미생물·생명공학회[DOI]
- Miller GL, "Use of dinitrosalicylic acid reagent for determination of reducing sugar", Anal. Chem, 31, pp.426~428 (1959)
- Moon YH, "Enzymatic synthesis and characterization of novel epigallocatechin gallate glucosides", J. Mol. Catal. B Enzym, 40, pp.1~7 (2006)
- Nguyen TT, "Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophiles and solubility enhancement of liquiritin and teniposide", Enzyme Microb. Technol, 64, pp.38~43 (2014)
- Ohtani K, "Further study on the 1,4-alphatransglucosylation of rubusoside, a sweet steviol-biglucoside from Rubus suavissimus", Agric. Biol. Chem, 55, pp.449~453 (1991)
- Seo ES, "Modified oligosaccharides as potential dental plaque control materials", Biotechnol. Prog, 20, pp.1550~1554 (2004)
- Su D, "Control of the synthesis of dextan and acceptor-products by Leuconosotc mesenteroides B-512FM dextransucrase", Carbohydr. Res, 248, pp.471~476 (1993)
- Tanaka O, "Improvement of taste of natural sweeteners", Pure Appl. Chem, 69, pp.675~683 (1997)
- Tanaka T, "Rubusoside (β-D-glucosyl ester of 13-O-β-D-glucosylsteviol), a sweet principle of Rubus chingii Hu (Rosaceae)", Agric. Biol. Chem, 45, pp.2165~2166 (1981)
- Yoon SH, "Synthesis of acarbose analogues by transglycosylation reactions of Leuconostoc mesenterioides B-512FMC and B-742CB dextransucrase", Carbohydr. Res, 24, pp.2427~2435 (2002)
- Zhang F, "Reformulation of etoposide with solubilityenhancing rubusoside. Int", J. Pharm, 434, pp.453~459 (2012)