Escherichia coli-Derived Uracil Increases the Antibacterial Activity and Growth Rate of Lactobacillus plantarum
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Lactobacillus plantarum (L. plantarum) is a representative probiotic. In particular, L. plantarum is the first commensal bacterium to colonize the intestine of infants. For this reason, the initial settlement of L. plantarum can play an important role in determining an infant’s health as well as their eventual health status as an adult. In addition, L. plantarum combats pathogenic infections (such as Escherichia coli (E. coli), one of the early pathogenic colonizers in an unhealthy infant gut) by secreting antimicrobial substances. The aim of this research was to determine how L. plantarum combats E. coli infection and why it is a representative probiotic in the intestine. Consequently, this research observed that E. coli releases uracil. L. plantarum specifically recognizes E. coli-derived uracil, which increases the growth rate and production of antimicrobial substance of L. plantarum. In addition, through the inhibitory activity test, this study postulates that the antimicrobial substance is a protein and can be considered a bacteriocin-like substance. Therefore, this research assumes that L. plantarum exerts its antibacterial ability by recognizing E. coli and increasing its growth rate as a result, and this phenomenon could be one of the reasons for L. plantarum settling in the intestine of infants as a beneficial bacterium.
Abstract
Lactobacillus plantarum (L. plantarum) is a representative probiotic. In particular, L. plantarum is the first commensal bacterium to colonize the intestine of infants. For this reason, the initial settlement of L. plantarum can play an important role in determining an infant’s health as well as their eventual health status as an adult. In addition, L. plantarum combats pathogenic infections (such as Escherichia coli (E. coli), one of the early pathogenic colonizers in an unhealthy infant gut) by secreting antimicrobial substances. The aim of this research was to determine how L. plantarum combats E. coli infection and why it is a representative probiotic in the intestine. Consequently, this research observed that E. coli releases uracil. L. plantarum specifically recognizes E. coli-derived uracil, which increases the growth rate and production of antimicrobial substance of L. plantarum. In addition, through the inhibitory activity test, this study postulates that the antimicrobial substance is a protein and can be considered a bacteriocin-like substance. Therefore, this research assumes that L. plantarum exerts its antibacterial ability by recognizing E. coli and increasing its growth rate as a result, and this phenomenon could be one of the reasons for L. plantarum settling in the intestine of infants as a beneficial bacterium.
참고문헌 (69)
- Atrih A, "Detection of bacteriocins produced by Lactobacillus plantarum strains isolated from different foods", Microbios, 75, pp.117~123 (1993)
- Baba T, "Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection", Mol. Systems Biol, 2, pp.2006~2008 (2006)
- Bedford Russell AR, "Could peripartum antibiotics have delayed health consequences for the infant", BJOG, 113, pp.758~765 (2006)
- Benor S, "Probiotic supplementation in mothers of very low birth weight infants", Am. J. Perinatol, 31, pp.497~504 (2014)
- Biasucci G, "Mode of delivery affects the bacterial community in the newborn gut", Early Hum. Dev, 86(Suppl. 1), pp.13~15 (2010)
- Bjorksten B, "The gut microbiota: a complex ecosystem. Clinical and experimental allergy", Clin. Exper. Allergy, 36, pp.1215~1217 (2006)
- Bjorksten B, "The intestinal microflora in allergic Estonian and Swedish 2-year-old children", Clin. Exper. Allergy, 29, pp.342~346 (1999)
- Bjorksten B, "Allergy development and the intestinal microflora during the first year of life", J. Allergy Clin. Immunol, 108, pp.516~520 (2001)
- Bours MJ, "Adenosine 5’-triphosphate and adenosine as endogenous signaling molecules in immunity and inflammation", Pharmacol. Ther, 112, pp.358~404 (2006)
- Debley JS, "Childhood asthma hospitalization risk after cesarean delivery in former term and premature infants", Ann. Allergy Asthma Immunol, 94, pp.228~233 (2005)
- Duncan SH, "Probiotics and prebiotics and health in ageing populations", Maturitas, 75, pp.44~50 (2013)
- Dunne C, "In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings", Am. J. Clin. Nutr, 73, pp.386S~392S (2001)
- Eckburg PB, "Diversity of the human intestinal microbial flora", Science, 308, pp.1635~1638 (2005)
- Eltzschig HK, "Neutrophils as sources of extracellular nucleotides: functional consequences at the vascular interface", Trends Cardiovasc. Med, 18, pp.103~107 (2008)
- Eltzschig HK, "Purinergic signaling during inflammation", N. Engl. J. Med, 367, pp.2322~2333 (2012)
- Fallani M, "Determinants of the human infant intestinal microbiota after the introduction of first complementary foods in infant samples from five European centres", Microbiology, 157, pp.1385~1392 (2011)
- Fukuda S, "Allergic symptoms and microflora in schoolchildren", J. Adolesc. Health, 35, pp.156~158 (2004)
- Gamelin E, "Rapid and sensitive high-performance liquid chromatographic analysis of halogenopyrimidines in plasma", J. Chromatogr. B Biomed. Sci. Appl, 695, pp.409~416 (1997)
- Gewolb IH, "Stool microflora in extremely low birthweight infants", Arch. Dis. Child. Fetal Neonatal Ed, 80, pp.F167~F173 (1999)
- Gibson GR, "Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics", J. Nutrit, 125, pp.1401~1412 (1995)
- Gronlund MM, "Fecal microflora in healthy infants born by different methods of delivery: permanent changes in intestinal flora after cesarean delivery", J. Pediatric Gastroenterol. Nutr, 28, pp.19~25 (1999)
- 하은미, "세균 배양액과 반응한 Lactobacillus plantarum의 생장속도 및 항균 활성에 대한 연구", 한국미생물·생명공학회지, 43(3), pp.187~194 (2015), 한국미생물·생명공학회[DOI]
- Hallstrom M, "Effects of mode of delivery and necrotising enterocolitis on the intestinal microflora in preterm infants", Eur. J. Clin. Microbiol. Infect. Dis, 23, pp.463~470 (2004)
- Iebba V, "Gut microbiota and pediatric disease", Dig. Dis, 29, pp.531~539 (2011)
- Isolauri E, "Probiotics in the management of atopic eczema", Clin. Exper. Allergy, 30, pp.1604~1610 (2000)
- Isolauri E, "A human Lactobacillus strain (Lactobacillus casei sp strain GG) promotes recovery from acute diarrhea in children", Pediatrics, 88, pp.90~97 (1991)
- Jack RW, "Bacteriocins of grampositive bacteria", Microbiol. Rev, 59, pp.171~200 (1995)
- Junger WG, "Immune cell regulation by autocrine purinergic signalling", Nat. Rev. Immunol, 11, pp.201~212 (2011)
- Kalliomaki M, "Pandemic of atopic diseases - a lack of microbial exposure in early infancy", Curr. Drug Targets Infect. Disord, 2, pp.193~199 (2002)
- Kalliomaki M, "Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing", J. Allergy Clin. Immunol, 107, pp.129~134 (2001)
- Kalliomaki M, "Probiotics in primary prevention of atopic disease: a randomised placebo-controlled trial", Lancet, 357, pp.1076~1079 (2001)
- Kelly D, "Importance of microbial colonization of the gut in early life to the development of immunity", Mutat. Res, 622, pp.58~69 (2007)
- Koizumi S, "UDP acting at P2Y6receptors is a mediator of microglial phagocytosis", Nature, 446, pp.1091~1095 (2007)
- Kreda SM, "Coordinated release of nucleotides and mucin from human airway epithelial Calu-3cells", J. Physiol, 584, pp.245~259 (2007)
- Kukkonen K, "Probiotics and prebiotic galacto-oligosaccharides in the prevention of allergic diseases:a randomized, double-blind, placebo-controlled trial", J. Allergy Clin. Immunol, 119, pp.192~198 (2007)
- Lang JR, "A comprehensive method validation strategy for bioanalytical applications in the pharmaceutical industry. Experimental considerations", J. Pharm. Biomed. Anal, 9, pp.357~361 (1991)
- Laubereau B, "Caesarean section and gastrointestinal symptoms, atopic dermatitis, and sensitization during the first year of life", Arch. Dis. Child, 89, pp.993~997 (2004)
- Lazarowski ER, "Vesicular and conductive mechanisms of nucleotide release", Purinergic Signal, 8, pp.359~373 (2012)
- Lazarowski ER, "UTP as an extracellular signaling molecule", News Physiol. Sci, 16, pp.1~5 (2001)
- Lazarowski ER, "Mechanisms of release of nucleotides and integration of their action as P2X- and P2Y-receptor activating molecules", Mol. Pharmacol, 64, pp.785~795 (2003)
- Lazarowski ER, "Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation", J. Biol. Chem, 272, pp.24348~24354 (1997)
- Lee KA, "Bacterial-derived uracil as a modulator of mucosal immunity and gut-microbe homeostasis in Drosophila", Cell, 153, pp.797~811 (2013)
- Lewus CB, "Inhibition of food-borne bacterial pathogens by bacteriocins from lactic acid bacteria isolated from meat", Appl. Environ. Microbiol, 57, pp.1683~1688 (1991)
- Lohner S, "Prebiotics in healthy infants and children for prevention of acute infectious diseases: a systematic review and metaanalysis", Nutr. Rev, 72, pp.523~531 (2014)
- Mackie RI, "Developmental microbial ecology of the neonatal gastrointestinal tract", Am. J. Clin. Nutr, 69, pp.1035S~1045S (1999)
- Majamaa H, "Probiotics: a novel approach in the management of food allergy", J. Allergy Clin. Immunol, 99, pp.179~185 (1997)
- Maldonado A, "Production of plantaricin NC8 by Lactobacillus plantarum NC8 is induced in the presence of different types of gram-positive bacteria", Arch. Microbiol, 181, pp.8~16 (2004)
- Masters M, "The pcnB gene of Escherichia coli, which is required for ColE1 copy number maintenance, is dispensable", J. Bacteriol, 175, pp.4405~4413 (1993)
- Mazmanian SK, "An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system", Cell, 122, pp.107~118 (2005)
- Messi P, "Detection and preliminary characterization of a bacteriocin (plantaricin 35d) produced by a Lactobacillus plantarum strain", Int. J. Food Microbiol, 64, pp.193~198 (2001)
- Negele K, "Mode of delivery and development of atopic disease during the first 2 years of life", Pediatr. Allergy Immunol, 15, pp.48~54 (2004)
- Orrhage K, "Factors controlling the bacterial colonization of the intestine in breastfed infants", Acta Paediatr. Suppl, 88, pp.47~57 (1999)
- Palmer C, "Development of the human infant intestinal microbiota", PLoS Biol, 5, pp.e177~ (2007)
- Penders J, "Gut microbiota composition and development of atopic manifestations in infancy: the KOALA Birth Cohort Study", Gut, 56, pp.661~667 (2007)
- Qin J, "A human gut microbial gene catalogue established by metagenomic sequencing", Nature, 464, pp.59~65 (2010)
- Rautava S, "The development of gut immune responses and gut microbiota: effects of probiotics in prevention and treatment of allergic disease", Curr. Issues Intest. Microbiol, 3, pp.15~22 (2002)
- Rinas U, "Entry of Escherichia coli into stationary phase is indicated by endogenous and exogenous accumulation of nucleobases", Appl. Environ. Microbiol, 61, pp.4147~4151 (1995)
- Rook GA, "Microbes, immunoregulation, and the gut", Gut, 54, pp.317~320 (2005)
- Ruiz-Barba JL, "Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducerregulated bacteriocin producer, in olive fermentations", Food Microbiol, 27, pp.413~417 (2010)
- Salazar N, "The human intestinal microbiome at extreme ages of life. Dietary intervention as a way to counteract alterations", Front. Genet, 5, pp.406~ (2014)
- Salminen S, "Influence of mode of delivery on gut microbiota composition in seven year old children", Gut, 53, pp.1388~1389 (2004)
- Hakimeh Sharafi, "Antibacterial Activity and Probiotic Potential of Lactobacillus plantarum HKN01: A New Insight into the Morphological Changes of Antibacterial Compound-Treated Escherichia coli by Electron Microscopy", Journal of Microbiology and Biotechnology, 23(2), pp.225~236 (2013), 한국미생물·생명공학회[DOI]
- Thompson-Chagoyan OC, "Aetiology of inflammatory bowel disease (IBD): role of intestinal microbiota and gut-associated lymphoid tissue immune response", Clin. Nutr, 24, pp.339~352 (2005)
- Ueda A, "Uracil influences quorum sensing and biofilm formation in Pseudomonas aeruginosa and fluorouracil is an antagonist", Microb. Biotechnol, 2, pp.62~74 (2009)
- Serpil Ugras, "Purification and Characterization of the Bacteriocin Thuricin Bn1 Produced by Bacillus thuringiensis subsp. kurstaki Bn1 Isolated from a Hazelnut Pest", Journal of Microbiology and Biotechnology, 23(2), pp.167~176 (2013), 한국미생물·생명공학회[DOI]
- van Reenen CA, "Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced by Lactobacillus plantarum", J. Appl. Microbiol, 84, pp.1131~1137 (1998)
- Weber TK, "Gastrointestinal microbiota and some children diseases: a review", Gastroenterol. Res. Pract, 2012, pp.676585~ (2012)
- Westerbeek EA, "The intestinal bacterial colonisation in preterm infants: a review of the literature", Clin. Nutr, 25, pp.361~368 (2006)
- Wihlborg AK, "Positive inotropic effects by uridine triphosphate (UTP) and uridine diphosphate (UDP) via P2Y2 and P2Y6receptors on cardiomyocytes and release of UTP in man during myocardial infarction", Circ. Res, 98, pp.970~976 (2006)
