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Curcumin Blocks Naproxen-Induced Gastric Antral Ulcerations through Inhibition of Lipid Peroxidation and Activation of Enzymatic Scavengers in Rats

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초록

Curcumin is a polyphenol derived from the plant Curcuma longa, which is used for the treatment of diseases associated with oxidative stress and inflammation. The present study was undertaken to determine the protective effect of curcumin against naproxen-induced gastric antral ulcerations in rats. Different doses (10, 50, and 100 mg/kg) of curcumin or vehicle (curcumin, 0 mg/kg) were pretreated for 3 days by oral gavage, and then gastric mucosal lesions were caused by 80 mg/kg naproxen applied for 3 days. Curcumin significantly inhibited the naproxen-induced gastric antral ulcer area and lipid peroxidation in a dose-dependent manner. In addition, curcumin markedly increased activities of radical scavenging enzymes, such as superoxide dismutase (SOD), catalase, and glutathione peroxidase in a dose-dependent manner. Specifically, 100 mg/kg curcumin completely protected the gastric mucosa against the loss in the enzyme, resulting in a drastic increase of activities of radical scavenging enzymes up to more than the level of untreated normal rats. Histological examination obviously showed that curcumin prevents naproxen-induced gastric antral ulceration as a result of direct protection of the gastric mucosa. These results suggest that curcumin blocks naproxen-induced gastric antral ulcerations through prevention of lipid peroxidation and activation of radical scavenging enzymes, and it may offer a potential remedy of gastric antral ulcerations.

Abstract

Curcumin is a polyphenol derived from the plant Curcuma longa, which is used for the treatment of diseases associated with oxidative stress and inflammation. The present study was undertaken to determine the protective effect of curcumin against naproxen-induced gastric antral ulcerations in rats. Different doses (10, 50, and 100 mg/kg) of curcumin or vehicle (curcumin, 0 mg/kg) were pretreated for 3 days by oral gavage, and then gastric mucosal lesions were caused by 80 mg/kg naproxen applied for 3 days. Curcumin significantly inhibited the naproxen-induced gastric antral ulcer area and lipid peroxidation in a dose-dependent manner. In addition, curcumin markedly increased activities of radical scavenging enzymes, such as superoxide dismutase (SOD), catalase, and glutathione peroxidase in a dose-dependent manner. Specifically, 100 mg/kg curcumin completely protected the gastric mucosa against the loss in the enzyme, resulting in a drastic increase of activities of radical scavenging enzymes up to more than the level of untreated normal rats. Histological examination obviously showed that curcumin prevents naproxen-induced gastric antral ulceration as a result of direct protection of the gastric mucosa. These results suggest that curcumin blocks naproxen-induced gastric antral ulcerations through prevention of lipid peroxidation and activation of radical scavenging enzymes, and it may offer a potential remedy of gastric antral ulcerations.

참고문헌 (37)

  1. Aebi H, "Methods of Enzymatic Analysis", pp.674~678 (1974), Academic Press
  2. Ajaikumar BK, "Curcumin potentiates antitumor activity of gemcitabine in an orthotopic model of pancreatic cancer through suppression of proliferation, angiogenesis, and inhibition of nuclear factor-κB-regulated gene products", Cancer Res, 67, pp.3853~3861 (2007)
  3. Ashish MK, "Curcumin potentiates the apoptosis effects of chemotherapeutic agents and cytokines through down-regulation of nuclear factor-κB and nuclear factor-κB-regulated gene products in IFN-α-sensitive and IFN-α-resistant human bladder cancer cells", Mol. Cancer Ther, 6, pp.1022~1030 (2007)
  4. Beck WS, "Gastrointestinal ulcerations induced by anti-inflammatory drugs in rats", Arch. Toxicol, 64, pp.210~217 (1990)
  5. Calhoun W, "Interaction studies of tilomisole, aspirin and naproxen in acute and chronic inflammation with assessment of gastrointestinal irritancy in the rat", Agents Actions, 36, pp.99~106 (1992)
  6. Cioli V, "The role of direct tissue contact in the production of gastrointestinal ulcers by anti-inflammatory drugs in rats", Toxicol. Appl. Pharmacol, 50, pp.283~289 (1979)
  7. Glienke W, "Wilms’tumour gene 1 (WT1) as a target in curcumin treatment of pancreatic cancer cells", Eur. J. Cancer, 45, pp.874~880 (2009)
  8. Half E, "Colon cancer: preventive agents and the present status of chemoprevention", Expert Opin. Pharmacother, 10, pp.211~219 (2009)
  9. Hogeboom GH, "Methods in Enzymology", pp.16~19 (1955), Academic Press
  10. Huang MT, "Inhibitory effects of curcumin on in vitro lipoxygenase and cyclooxygenase activities in mouse epidermis", Cancer Res, 51, pp.813~819 (1991)
  11. Jiao Y, "Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator", Blood, 113, pp.462~469 (2009)
  12. Kang J, "Curcumininduced histone hypoacetylation: the role of reactive oxygen species", Biochem. Pharmacol, 69, pp.1205~1213 (2005)
  13. Kim JH, "Protective effect of astaxanthin on naproxen-induced gastric antral ulceration in rats", Eur. J. Pharmacol, 514, pp.53~59 (2005)
  14. Krishnan MD, "Curcumin suppresses growth and chemoresistance of human glioblastoma cells via AP-1 and NF-κB transcription factors", J. Neurochem, 102, pp.522~538 (2007)
  15. Lawrence RA, "Glutathione peroxidase activity in selenium-deficient rat liver", Biochem. Biophys. Res. Commun, 71, pp.952~958 (1976)
  16. Lin YG, "Curcumin inhibits tumor growth and angiogenesis in ovarian carcinoma by targeting the nuclear factor-κB pathway", Clin. Cancer. Res, 13, pp.3423~3430 (2007)
  17. Liu E, "Curcumin induces G2", M cell cycle arrest in a p53-dependent manner and upregulates ING4 expression in human glioma, 2007, pp.85, 263~270
  18. Lodha R, "Traditional Indian systems of medicine", Ann. Acad. Med. Singapore, 29, pp.37~41 (2000)
  19. McCarthy DM, "NSAID-induced gastro-intestinal damage - a critical review of prophylaxis and therapy", J. Clin. Gastroenterol, 12, pp.S13~S20 (1990)
  20. McCarthy DM, "Mechanisms of mucosal injury and healing: the role of nonsteroidal anti-inflammatory drugs", Scand. J. Gastroenterol, 208, pp.24~29 (1995)
  21. McCord JM, "Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein)", J. Biol. Chem, 244, pp.6049~6055 (1967)
  22. Milacic V, "Curcumin inhibits the proteasome activity in human colon cancer cells in vitro and in vivo", Cancer Res, 68, pp.7283~7292 (2008)
  23. Ohkawa H, "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction", Anal. Biochem, 95, pp.351~358 (1979)
  24. Parks DA, "Oxygen radicals: mediators of gastrointestinal pathophysiology", Gut, 30, pp.293~298 (1989)
  25. Roth SH, "Non-steroidal anti-inflammatory drug gastropathy: recognition and response", Arch. Intern. Med, 147, pp.2093~2100 (1987)
  26. Ruby AJ, "Anti-tumour and antioxidant activity of natural curcuminoids", Cancer Lett, 94, pp.79~83 (1995)
  27. Salvioli S, "Curcumin in cell death processes: a challenge for CAM of age-related pathologies", Evid. Based Complement. Alternat. Med, 4, pp.181~190 (2007)
  28. Sankar B, "Curcumin prevents tumor-induced T cell apoptosis through Stat-5a-mediated Bcl-2 induction", J. Biol. Chem, 282, pp.15954~15964 (2007)
  29. Shishodia S, "Curcumin: getting back to the roots", Ann. NY Acad. Sci, 1056, pp.206~217 (2005)
  30. Surajit K, "Curcumin suppressed anti-apoptotic signals and activated cysteine proteases for apoptosis in human malignant glioblastoma U87MG cells", Neurochem. Res, 32, pp.2103~2113 (2007)
  31. Suwa T, "Comparative studies on the gastrointestinal lesions caused by several nonsteroidal anti-inflammatory agents in rats", Agents Actions, 21, pp.167~172 (1987)
  32. Swamy MV, "Prevention and treatment of pancreatic cancer by curcumin in combination with omega-3 fatty acids", Nutr. Cancer, 60, pp.81~89 (2008)
  33. Thiyagarajan M, "Neuroprotective effect of curcumin in middle cerebral artery occlusion induced focal cerebral ischemia in rats", Life Sci, 74, pp.969~985 (2004)
  34. Wang Q, "Neuroprotective mechanisms of curcumin against cerebral ischemia-induced neuronal apoptosis and behavioral deficits", J. Neurosci. Res, 82, pp.138~148 (2005)
  35. Yang F, "The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse", J. Biol. Chem, 280, pp.5892~5901 (2005)
  36. Yoshikawa T, "Role of oxygen-derived free radicals in the pathogenesis of gastric mucosal lesions in rats", J. Clin. Gastroenterol, 12, pp.65~71 (1990)
  37. Yu Z, "Curcumin down-regulates Ets-1 and Bcl-2 expression in human endometrial carcinoma HEC-1-A cells", Gynecol. Oncol, 103, pp.541~548 (2007)

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