Biodegradation of Ochratoxin A by Aspergillus tubingensis Isolated from Meju
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Ochratoxin A (OTA), a mycotoxin, contaminates agricultural products and poses a serious threat to public health worldwide. Microbiological methods are known to be a promising approach for OTA biodegradation because physical and chemical methods have practical limitations. In the present study, a total of 130 fungal isolates obtained from 65 traditional Korean meju (a fermented starter for fermentation of soybeans) samples were examined for OTA-biodegradation activity using thin-layer chromatography. Two fungal isolates were selected for OTA-biodegradation activity and were identified as Aspergillus tubingensis M036 and M074 through sequence analysis of the beta-tubulin gene. After culturing both A. tubingensis isolates in Soytone-Czapek medium containing OTA (40 ng/ml), OTAbiodegradation activity was analyzed using high-performance liquid chromatography (HPLC). Both A. tubingensis strains degraded OTA by more than 95.0% after 14 days, and the HPLC analysis showed that the OTA biodegradation by the A. tubingensis strains led to the production of ochratoxin α, which is much less toxic than OTA. Moreover, crude enzymes from the cultures of A. tubingensis M036 and M074 led to OTA biodegradation of 97.5% and 91.3% at pH 5, and 80.3% and 75.3% at pH 7, respectively, in a buffer solution containing OTA (40 ng/ml) after 24 h. In addition, the OTA-biodegrading fungi did not exhibit OTA production activity. Our data suggest that A. tubingensis isolates and their enzymes have the potential for practical application to reduce levels of OTA in food and feed.
Abstract
Ochratoxin A (OTA), a mycotoxin, contaminates agricultural products and poses a serious threat to public health worldwide. Microbiological methods are known to be a promising approach for OTA biodegradation because physical and chemical methods have practical limitations. In the present study, a total of 130 fungal isolates obtained from 65 traditional Korean meju (a fermented starter for fermentation of soybeans) samples were examined for OTA-biodegradation activity using thin-layer chromatography. Two fungal isolates were selected for OTA-biodegradation activity and were identified as Aspergillus tubingensis M036 and M074 through sequence analysis of the beta-tubulin gene. After culturing both A. tubingensis isolates in Soytone-Czapek medium containing OTA (40 ng/ml), OTAbiodegradation activity was analyzed using high-performance liquid chromatography (HPLC). Both A. tubingensis strains degraded OTA by more than 95.0% after 14 days, and the HPLC analysis showed that the OTA biodegradation by the A. tubingensis strains led to the production of ochratoxin α, which is much less toxic than OTA. Moreover, crude enzymes from the cultures of A. tubingensis M036 and M074 led to OTA biodegradation of 97.5% and 91.3% at pH 5, and 80.3% and 75.3% at pH 7, respectively, in a buffer solution containing OTA (40 ng/ml) after 24 h. In addition, the OTA-biodegrading fungi did not exhibit OTA production activity. Our data suggest that A. tubingensis isolates and their enzymes have the potential for practical application to reduce levels of OTA in food and feed.
참고문헌 (30)
- Abrunhosa L, "Biodegradation of ochratoxin A by fungi isolated from grapes", J. Agric. Food Chem, 50, pp.7493~7496 (2002)
- Abrunhosa L, "Isolation and purification of an enzyme hydrolyzing ochratoxin A from Aspergillus niger", Biotechnol. Lett, 29, pp.1909~1914 (2007)
- Alexander J, "Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to ochratoxin A in food", EFSA J, 365, pp.1~56 (2006)
- Bejaoui H, "Biodegradation of ochratoxin A by Aspergillus section Nigri species isolated from French grapes: a potential means of ochratoxin A decontamination in grape juices and musts", FEMS Microbiol. Lett, 255, pp.203~208 (2006)
- Chang X, "Degradation of ochratoxin A by Bacillus amyloliquefaciens ASAG1", Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess, 32, pp.564~571 (2015)
- Dachoupakan C, "Study of the phenotypic and genotypic biodiversity of potentially ochratoxigenic black aspergilli isolated from grapes", Int. J. Food Microbiol, 132, pp.14~23 (2009)
- FAO, "WHO (Food and Agricultural Organization", World Health Organization), Prepared by the Fifty-Sixth Meeting of the Joint FAO, pp.WHO Expert Committee on Food Additives, 2001[DOI]
- Ferenczi S, "A new ochratoxin A biodegradation strategy using Cupriavidus basilensis Or16 strain", PLoS One, 9, pp.e109817~ (2014)
- Gil-Serna J, "Revision of ochratoxin A production capacity by the main species of Aspergillus section Circumdati. Aspergillus steynii revealed as the main risk of OTA contamination", Food Control, 22, pp.343~345 (2011)
- Glass NL, "Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes", Appl. Environ. Microbiol, 61, pp.1323~1330 (1995)
- He C, "Isolation and identification of a strain of Aspergillus tubingensis with deoxynivalenol biotransformation capability", Int. J. Mol. Sci, 9, pp.2366~2375 (2008)
- Huwig A, "Mycotoxin detoxication of animal feed by different adsorbents", Toxicol. Lett, 122, pp.179~188 (2001)
- IARC Working Group on the Evaluation of Carcinogenic Risks to Humans, "Some traditional herbal medicines, some mycotoxins, naphthalene and styrene", IARC Monogr. Eval. Carcinog. Risks Hum, 82, pp.1~556 (2002)
- Yu Jung Jung, "Isolation and Identification of Fungi from a Meju Contaminated with Aflatoxins", Journal of Microbiology and Biotechnology, 22(12), pp.1740~1748 (2012), 한국미생물·생명공학회[DOI]
- McKenzie K, "Oxidative degradation and detoxification of mycotoxins using a novel source of ozone", Food Chem. Toxicol, 35, pp.807~820 (1997)
- Medina A, "Study of Spanish grape mycobiota and ochratoxin A production by isolates of Aspergillus tubingensis and other members of Aspergillus section Nigri", Appl. Environ. Microbiol, 71, pp.4696~4702 (2005)
- Rodriguez H, "Degradation of ochratoxin A by Brevibacterium species", J. Agric. Food Chem, 59, pp.10755~10760 (2011)
- Samson RA, "Phylogeny, identification and nomenclature of the genus Aspergillus", Stud. Mycol, 78, pp.141~173 (2014)
- Sartori D, "PCR method for the detection of potential ochratoxinproducing Aspergillus species in coffee beans", Res. Microbiol, 157, pp.350~354 (2006)
- Schatzmayr G, "Evidence of ochratoxin A-detoxification activity of rumen fluid, intestinal fluid and soil samples as well as isolation of relevant microorganisms from these environments", Mycotoxin Res, 18(Suppl 2), pp.183~187 (2002)
- Schatzmayr G, "Microbiologicals for deactivating mycotoxins", Mol. Nutr. Food Res, 50, pp.543~551 (2006)
- Schuster E, "On the safety of Aspergillus niger – a review", Appl. Microbiol. Biotechnol, 59, pp.426~435 (2002)
- Shi L, "Ochratoxin A biocontrol and biodegradation by Bacillus subtilis CW 14", J. Sci. Food Agric, 94, pp.1879~1885 (2014)
- Skrinjar M, "Lowering of ochratoxin A level in milk by yoghurt bacteria and bifidobacteria", Folia Microbiol. (Praha), 41, pp.26~28 (1996)
- Stander MA, "A kinetic study into the hydrolysis of the ochratoxins and analogues by carboxypeptidase A", Chem. Res. Toxicol, 14, pp.302~304 (2001)
- Storari M, "Isolation of mycotoxins producing black aspergilli in herbal teas available on the Swiss market", Food Control, 26, pp.157~161 (2012)
- Tamura K, "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol. Biol. Evol, 28, pp.2731~2739 (2011)
- Varga J, "Degradation of ochratoxin A and other mycotoxins by Rhizopus isolates", Int. J. Food Microbiol, 99, pp.321~328 (2005)
- Wang Y, "Ochratoxin A producing fungi, biosynthetic pathway and regulatory mechanisms", Toxins (Basel), 8, pp.E83~ (2016)
- Xiao H, "Metabolites of ochratoxins in rat urine and in a culture of Aspergillus ochraceus", Appl. Environ. Microbiol, 62, pp.648~655 (1996)