본문 바로가기
KERD
논문KCI 등재

Isolation, Identification, and Expression of Microbial Cellulases from the Gut of Odontotermes formosanus

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류생물학

초록

Termites are destructive to agriculture, forestry, and buildings, but they can also promote agro-ecosystem balance through the degradation of lignocellulose. Termite-triggered cellulose digestion may be clarified through microbial metabolism of cellulose products. In the present study, we characterized the activities of cellulase and its three components synthesized by the cellulase-producing fungal strain HDZK-BYTF620 isolated from the gut of Odontotermes formosanus. The protein components of cellulases were synthesized by strain HDZK-BYTF620, which were isolated and characterized using polyacrylamide gel electrophoresis, and the expression of the cellulases was studied at the proteome level.

Abstract

Termites are destructive to agriculture, forestry, and buildings, but they can also promote agro-ecosystem balance through the degradation of lignocellulose. Termite-triggered cellulose digestion may be clarified through microbial metabolism of cellulose products. In the present study, we characterized the activities of cellulase and its three components synthesized by the cellulase-producing fungal strain HDZK-BYTF620 isolated from the gut of Odontotermes formosanus. The protein components of cellulases were synthesized by strain HDZK-BYTF620, which were isolated and characterized using polyacrylamide gel electrophoresis, and the expression of the cellulases was studied at the proteome level.

참고문헌 (32)

  1. Abe T, "Termites: Evolution, Sociality, Symbioses, Ecology" (2000), Kluwer Academic Publishers
  2. Mattéotti C, "Identification and characterization of a new xylanase from gram-positive bacteria isolated from termite gut (Reticulitermes santonensis)", Protein Express. Purif, 83, pp.117~127 (2012)
  3. Zhou XG, "Social exploitation of hexamerin: RNAi reveals a major caste-regulatory factor in termites", Proc. Natl. Acad. Sci. USA, 103, pp.4499~4504 (2006)
  4. Scharf ME, "Termite digestomes as sources for novel lignocellulases", Biofuels Bioprod. Biorefin, 2, pp.540~552 (2008)
  5. Scharf ME, "Gene expression profiles among immature and adult reproductive castes of the termite Reticulitermes flavipes", Insect Mol. Biol, 14, pp.31~44 (2005)
  6. Watanabe H, "Animal cellulases", Cell. Mol. Life Sci, 58, pp.1167~1178 (2001)
  7. Husseneder C, "Transcriptome profiling of female alates and egglaying queens of the Formosan subterranean termite", Comp. Biochem. Phys. C, 7, pp.14~27 (2012)
  8. Chaffron S, "Termites in the wood work", Genome Biol, 8, pp.229~ (2007)
  9. Droge S, "Spirochaeta coccoides sp. nov., a novel coccoid spirochete from the hindgut of the termite Neotermes castaneus", Appl. Environ. Microbiol, 72, pp.392~397 (2006)
  10. Ohkuma M., "Termite symbiotic systems: efficient biorecycling of lignocellulose", Appl. Microbiol. Biotechnol, 61, pp.1~9 (2003)
  11. Wenzel M, "Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticolli", J. Appl. Microbiol, 92, pp.32~40 (2002)
  12. Yamada A, "Carbon mineralization by termites in tropical forests, with emphasis on fungus combs", Ecol. Res, 20, pp.453~460 (2005)
  13. Nia J, "Identification of activity related amino acid mutations of GH9 termite cellulose", Bioresour. Technol, 101, pp.6438~6443 (2010)
  14. Breznak JA, "Role of microorganisms in the digestion of lignocellulose by termites", Ann. Rev. Entomol, 39, pp.453~487 (1994)
  15. Rouland-Lefevre C, "Large-scale identification of transcripts expressed in a symbiotic fungus (Termitomyces)during plant biomass degradation", Appl. Microbiol. Biotechnol, 73, pp.195~203 (2006)
  16. Dong C, "Pathogenicity of a new China variety of Metarhizium anisopliae (M. anisopliae var. dcjhyium) to subterranean termite Odontotermes formosanus", Microbiol. Res, 164, pp.27~35 (2007)
  17. Li H, "Physicochemical conditions and metal ion profiles in the gut of the fungus-growing termite Odontotermes formosanus", J. Insect Physiol, 58, pp.1368~1375 (2012)
  18. Huang Q, "Characterization of head transcriptome and analysis of gene expression involved in caste differentiation and aggression in Odontotermes formosanus (Shiraki)", PLoS One, 7, pp.e50383~ (2012)
  19. Dopson M, "Analysis of differential protein expression during growth states of Ferroplasma strains and insights into electron transport for iron oxidation", Microbiology, 151, pp.4127~4137 (2005)
  20. Zhu MJ, "Studies in proteases distribution of golden fish digestive with refolding electrophoresis", Food Sci. Technol. Res, 2, pp.114~117 (2010)
  21. Chen SX, "Rapid protein identification using direct infusion nanoelectrospray ionization mass spectrometry", Proteomics, 6, pp.16~25 (2006)
  22. Brent CS, "Effects of social stimuli on the endocrine systems of maturing queens of the dampwood termite Zootermopsis angusticollis", Physiol. Entomol, 32, pp.26~33 (2007)
  23. Maekawa K, "Juvenile hormone titer and vitellogenin gene expression related to ovarian development in primary reproductives compared with nymphs and nymphoid reproductives of the termite Reticulitermes speratus", Physiol. Entomol, 35, pp.52~58 (2010)
  24. Raina AK, "Structure of spermatheca, sperm dynamics and associated bacteria in Formosan subterranean termite (Isoptera: Rhinotermitidae)", Ann. Entomol. Soc. Am, 100, pp.418~424 (2007)
  25. Scharf ME, "Caste- and development-associated gene expression in a lower termite", Genome Biol, 4, pp.R62~ (2003)
  26. Tokuda G, "Major alteration of the expression site of endogenous cellulases in members of an apical termite lineage", Mol. Ecol, 13, pp.3219~3228 (2004)
  27. Liu Y, "Cellulase molecular biology and genetic engineering", Feed Ind, 18, pp.11~14 (2007)
  28. Qiao YH, "Analysis of recombinant protein aggregation with SDS-PAGE technology", Lab. Sci, 3, pp.64~66 (2007)
  29. Jeoh T, "Cooperative and competitive binding in synergistic mixtures of Thermobifida fusca cellulases Cel5A, Cel6B, and Cel9A", Biotechnol. Progr, 18, pp.760~769 (2002)
  30. Jeoh T, "Effect of cellulase mole fraction and cellulose recalcitrance on synergism in cellulose hydrolysis in binding", Biotechnol. Progr, 22, pp.270~277 (2006)
  31. Pribowo A, "The adsorption and enzyme activity profiles of specific Trichoderma reesei cellulose", xylanase components when hydrolyzing steam pretreated corn stover, 2012, pp.50, 195~203
  32. Sun WC, "Protein expression and enzymatic activity of cellulses produced by Trichoderma reesei Rut C-30 on rice straw", Process Biochem, 43, pp.1083~1087 (2008)

관련 논문AI추천

Molecular cloning, expression and characterization of a novel feruloyl esterase enzyme from the symbionts of termite (Coptotermes formosanus) gutAppoothy Thulasi, M Bagath, Chenniappan Palanivel, Duvvuri Prasanna Kumar, K.T. Sampath, Matam Chandrasekharaiah, Sunil Singh Santosh, Vazhakkala Lyju Jose생화학분자생물학회 · BMB Reports2011 · 제44권 제1호Effective Microwell Plate-Based Screening Method for Microbes Producing Cellulase and Xylanase and Its Application강도형, 권영경, 김지형, 오철홍, 이영득, 정원교, 허수진, 현정호, Jennifer Jooyoun Kim, Kae Kyoung Kwon, Su-Jin Lee, Won-Bo Shim한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2014 · 제24권 제11호Screening and Characterization of a Novel Cellulase Gene from the Gut Microflora of Hermetia illucens Using Metagenomic Library구본성, 윤상홍, 이창묵, 한범수, Joon-Soo Sim, So-Hyeon Seo, Soo-Jin Kim, Young-Seok Lee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2014 · 제24권 제9호Towards a Miniaturized Culture Screening for Cellulolytic Fungi and Their Agricultural Lignocellulosic DegradationCharuwan Chuaseeharonnachai, Chatuphon Siamphan, Jantima Arnthong, Nattawut Boonyuen, Surisa Suwannarangsee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제11호Metagenomic Analysis of the Fecal Microbiomes of Wild Asian Elephants Reveals Microflora and Enzymes that Mainly Digest HemicelluloseBo Xu, Chengbo Zhang, Tao Lu, ZUN-XI HUANG한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제8호Evaluation of Cellulolytic Enzyme Production by Indigenous Fungi in Korea김재진, 이영민, 이재정, 이한별, 허영목한국환경생물학회 · 환경생물2017 · 제35권 제4호

이 간행물의 다른 논문 (10건)