본문 바로가기
KERD
논문KCI 등재

Comparative Analysis of Gut Microbial Communities in Children under 5 Years Old with Diarrhea

2

조회

0

다운로드

3

인용

0

참고문헌

키워드

주제분류생물학

초록

Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children with diarrhea caused by dyspepsia and defined as the non-infectious group; and ten samples had been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by using high-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbes in samples of the infectious group was much higher than in the non-infectious group. In addition, the relative abundance of Enterococcus in the healthy control group was significantly higher than in the non-infectious group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.

Abstract

Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children with diarrhea caused by dyspepsia and defined as the non-infectious group; and ten samples had been taken from children with diarrhea induced by intestinal infections and identified as the infectious group. We detected the microbial communities of samples by using high-throughput sequencing of 16S rRNA genes. The proportion of aerobic and facultative anaerobic microbes in samples of the infectious group was much higher than in the non-infectious group. In addition, the relative abundance of Enterococcus in the healthy control group was significantly higher than in the non-infectious group and infectious group. This can be used as a potential diagnostic biomarker for diarrhea.

참고문헌 (43)

  1. Van Niel CW, "Lactobacillus therapy for acute infectious diarrhea in children: a meta-analysis", Pediatrics, 109, pp.678~684 (2002)[DOI]
  2. Walker CLF, "Estimating diarrhea mortality among young children in low and middle income countries", PLoS One, 7, pp.e29151~ (2012)[DOI]
  3. Qu M, "Etiology of acute diarrhea due to enteropathogenic bacteria in Beijing, China", J. Infect., 65, pp.214~222 (2012)[DOI]
  4. Colombara DV, "Chronic health consequences of acute enteric infections in the developing world", Am. J. Gastroenterol., 3, pp.12~24 (2016)[DOI]
  5. Harris VC, "The infant gut microbiome correlates significantly with rotavirus vaccine response in rural Ghana", J. Infect. Dis., 215, pp.34~41 (2017)[DOI]
  6. Kajbaf TZ, "Relative frequency of rotavirus and adenovirus among children aged 1-60 months hospitalized with acute diarrhoea in south-western, Iran", Jundishapur J. Microbiol., 6, pp.47~50 (2013)
  7. Kiulia NM, "Astrovirus infection in young Kenyan children with diarrhoea", J. Trop. Pediatr., 53, pp.206~209 (2007)[DOI]
  8. Castro TX, "Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil", Vet. J., 206, pp.115~117 (2015)[DOI]
  9. Randremanana R, "Case-control study of the etiology of infant diarrheal disease in 14 districts in Madagascar", PLoS One, 7, pp.e44533~ (2012)[DOI]
  10. Majowicz S E, "The global burden of nontyphoidal Salmonella gastroenteritis", Clin. Infect. Dis., 50, pp.882~889 (2010)[DOI]
  11. Tadesse G, "Prevalence of human salmonellosis in Ethiopia: a systematic review and meta-analysis", BMC Infect. Dis., 14, pp.88~ (2014)[DOI]
  12. Striepen B, "Parasitic infections: time to tackle cryptosporidiosis", Nature, 503, pp.189~191 (2013)[DOI]
  13. Tsuchiaka S, "Development of a novel detection system for microbes from bovine diarrhea by real-time PCR", J. Vet. Med. Sci., 78, pp.383~389 (2016)[DOI]
  14. Ley RE, "Ecological and evolutionary forces shaping microbial diversity in the human intestine", Cell, 124, pp.837~848 (2006)[DOI]
  15. Huang Z, "Changes in the intestinal bacterial community during the growth of white shrimp, Litopenaeus vannamei", Aquac. Res., 47, pp.1737~1746 (2016)[DOI]
  16. Tap J, "Towards the human intestinal microbiota phylogenetic core", Environ. Microbiol., 11, pp.2574~2584 (2009)[DOI]
  17. Christensen EG, "Dietary xylo-oligosaccharide stimulates intestinal bifidobacteria and lactobacilli but has limited effect on intestinal integrity in rats", BMC Res. Notes, 7, pp.660~ (2014)[DOI]
  18. Hao WL, "Microflora of the gastrointestinal tract: a review", Methods Mol. Biol., 268, pp.491~502 (2004)
  19. Reis RS, "Enteropathogenic Escherichia coli, Samonella, Shigella and Yersinia: cellular aspects of host-bacteria interactions in enteric diseases", Gut Pathog., 2, pp.8~ (2010)[DOI]
  20. Haque R, "Rapid diagnosis of Entamoeba infection by using Entamoeba and Entamoeba histolytica stool antigen detection kits", J. Clin. Microbiol., 33, pp.2558~2561 (1995)
  21. Kirby A, "An evaluation of the RIDASCREEN and IDEIA enzyme immunoassays and the RIDAQUICK immunochromatographic test for the detection of norovirus in faecal specimens", J. Clin. Virol., 49, pp.254~257 (2010)[DOI]
  22. Mardis ER, "Next-generation DNA sequencing methods", Annu. Rev. Genomics Hum. Genet., 9, pp.387~402 (2008)[DOI]
  23. Novais RC, "The evolution of pyrosequencing for microbiology: from genes to genomes", J. Microbiol. Methods, 86, pp.1~7 (2011)[DOI]
  24. Glimcher LH, "Homeostasis and inflammation in the intestine", Cell, 140, pp.859~870 (2010)[DOI]
  25. Emonet S, "Clinical metagenomics for the diagnostic of hospital-acquired infections: promises and hurdles", Am. J. Respir. Crit. Care Med., 196, pp.1617~1618 (2017)[DOI]
  26. Wang Y, "Antimicrobial blue light inactivation of pathogenic microbes: state of the art", Drug Resist. Updat., 33-35, pp.1~22 (2017)[DOI]
  27. Kumar GD, "Aeolian contamination of fruits by enteric pathogens: an unexplored paradigm", Curr. Opin. Food Sci. (2017)[DOI]
  28. Gao R, "Dysbiosis signatures of gut microbiota along the sequence from healthy, young patients to those with overweight and obesity", Obesity, 26, pp.351~361 (2017)
  29. Segata N, "Metagenomic biomarker discovery and explanation", Genome Biol., 12, pp.R60~ (2011)[DOI]
  30. Buret AG, "Good bugs, bad bugs in the gut: the role of microbiota dysbiosis in chronic gastrointestinal consequences of infection", Am. J. Gastroenterol., 3, pp.25~32 (2016)[DOI]
  31. Saha DR, "Inflammatory diarrhea due to enteroaggregative Escherichia coli: evidence from clinical and mice model studies", Gut. Pathog., 5, pp.36~ (2013)[DOI]
  32. Swidsinski A, "Biostructure of fecal microbiota in healthy subjects and patients with chronic idiopathic diarrhea", Gastroenterology, 135, pp.568~579 (2008)[DOI]
  33. Albert MJ, "Controlled study of Escherichia coli diarrheal infections in Bangladeshi children", J. Clin. Microbiol., 33, pp.973~977 (1995)
  34. Jin D, "Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis", BMC Microbiol., 13, pp.141~ (2013)[DOI]
  35. Segain J, "Butyrate inhibits inflammatory responses through NFkappaB inhibition: implications for Crohn's disease", Gut, 47, pp.397~403 (2000)[DOI]
  36. Louis P, "Diversity, metabolism and microbial ecology of butyrate-producing bacteria from the human large intestine", FEMS Microbiol. Lett., 294, pp.1~8 (2009)[DOI]
  37. Xin Y, "Study of enteropathogenic bacteria in children with acute diarrhoea aged from 7 to 10 years in Xuzhou, China", Microb. Pathog., 91, pp.41~45 (2015)
  38. Matamoros S, "Development of intestinal microbiota in infants and its impact on health", Trends Microbiol., 21, pp.167~173 (2013)[DOI]
  39. Palmer C, "Development of the human infant intestinal microbiota", PLoS Biol., 5, pp.e177~ (2007)[DOI]
  40. Jett BD, "Virulence of enterococci", Clin. Microbiol. Rev., 7, pp.462~478 (1994)[DOI]
  41. LI ZJ, "Application of 16S rDNA sequence analysis in the bacterial species survey of fecal samples from adult diarrheic patients", Chin. J. Zoonoses, 24, pp.100~103 (2008)
  42. LI JZ, "Clinical evaluation with live combined Bacillus subtilis and Enterococcus faecium combined with dimethicone on diarrhea-predominant irritable bowel syndrome", China Med. Herald, 5, pp.R574~ (2010)
  43. Bybee SN, "Effect of the probiotic Enterococcus faecium SF68 on presence of diarrhea in cats and dogs housed in an animal shelter", J. Vet. Intern. Med., 25, pp.856~860 (2011)[DOI]

관련 논문AI추천

Rare and Intermediate Taxa Shape the Gut Bacterial Structure in Neonates and Preterm Infants with Necrotizing EnterocolitisChang Fan, Gao Xuyang, Jia Fengan, Li Dan, Wang Qi, Wang Yu, Xie Yun, Zhang Xiaoge한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 Microbiome Study of Initial Gut Microbiota from Newborn Infants to Children Reveals that Diet Determines Its Compositional Development구혜진, 김유태, 이주훈한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2020 · 제30권 제7호Immunomodulatory Effects of Bifidobacterium spp. and Use of Bifidobacterium breve and Bifidobacterium longum on Acute Diarrhea in ChildrenChoi Yae Jin, Shin Hea Soon, Shin Seon-Hee한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2022 · 제32권 제9호Effect of Diet and Lifestyle Changes on Gut Microbial Diversity in Healthy AdolescentsChae Yeongjae, Choi Yejin, Doo Hyunok, Kang Juyoun, Keum Gi Beom, Kim Haram, Kim Hyeun Bum, Kim Sheena, Kwak Jinok, Lee Suyoung, Sun Xingmin, Yang Hyunjin, Yoo Soo Jin한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 The Effect of Oral Nutritional Supplements in Improving the Condition of Children with Diarrhea: A Systematic ReviewFateme Asadi Touranlou, Kamiar Mahmoudifar, Mehrara Hashempoor, Mitra Rezaie대한소아소화기영양학회 · Pediatric Gastroenterology, Hepatology & Nutrition2025 · 제28권 제5호Scarring the early-life microbiome: its potential life-long effects on human health and diseases고아라, 박나영, 박현지생화학분자생물학회 · BMB Reports2023 · 제56권 제9호

이 간행물의 다른 논문 (9건)