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Hepatitis E Virus Papain-Like Cysteine Protease Inhibits Type I Interferon Induction by Down-Regulating Melanoma Differentiation-Associated Gene 5

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초록

Upon viral infection, the host cell recognizes the invasion through a number of pattern recognition receptors. Melanoma differentiation associated gene 5 (MDA5) and retinoic acidinducible gene-I (RIG-I) recognize RNA molecules derived from invading viruses, activating down-stream signaling cascades, culminating in the induction of the type I interferon. On the other hand, viruses have evolved to evade type I interferon-mediated inhibition. Hepatitis E virus has been shown to encode a few antagonists of type I interferon and it is not surprising that viruses encode multiple mechanisms of viral evasion. In the present study, we demonstrated that HEV PCP strongly down-regulates MDA5-mediated activation of interferon β induction in a dose-dependent manner. Interestingly, MDA5 protein expression was almost completely abolished. In addition, polyinosinic polycytidylic acid (poly(I:C))- and Sendai virus-mediated activation of type I interferon responses were similarly abrogated in the presence of HEV PCP. Furthermore, HEV PCP down-regulates several molecules that play critical roles in the induction of type I IFN expression. Taken together, these data collectively suggest that HEV-encoded PCP is a strong antagonist of type I interferon.

Abstract

Upon viral infection, the host cell recognizes the invasion through a number of pattern recognition receptors. Melanoma differentiation associated gene 5 (MDA5) and retinoic acidinducible gene-I (RIG-I) recognize RNA molecules derived from invading viruses, activating down-stream signaling cascades, culminating in the induction of the type I interferon. On the other hand, viruses have evolved to evade type I interferon-mediated inhibition. Hepatitis E virus has been shown to encode a few antagonists of type I interferon and it is not surprising that viruses encode multiple mechanisms of viral evasion. In the present study, we demonstrated that HEV PCP strongly down-regulates MDA5-mediated activation of interferon β induction in a dose-dependent manner. Interestingly, MDA5 protein expression was almost completely abolished. In addition, polyinosinic polycytidylic acid (poly(I:C))- and Sendai virus-mediated activation of type I interferon responses were similarly abrogated in the presence of HEV PCP. Furthermore, HEV PCP down-regulates several molecules that play critical roles in the induction of type I IFN expression. Taken together, these data collectively suggest that HEV-encoded PCP is a strong antagonist of type I interferon.

참고문헌 (50)

  1. Forni D, "Origin and dispersal of Hepatitis E virus", Emerg. Microbes Infect, 7, pp.11~ (2018)
  2. Tam AW, "Hepatitis E virus(HEV) : molecular cloning and sequencing of the full-length viral genome", Virology, 185, pp.120~131 (1991)
  3. 강상민, "Host Innate Immunity against Hepatitis E Virus and Viral Evasion Mechanisms", Journal of Microbiology and Biotechnology, 27(10), pp.1727~1735 (2017), 한국미생물·생명공학회
  4. Kumar S, "Hepatitis E virus: the current scenario", Int. J. Infect. Dis, 17, pp.e228~e233 (2013)
  5. Krawczynski K, "Hepatitis E", Hepatology, 17, pp.932~941 (1993)
  6. Wong DC, "Epidemic and endemic hepatitis in India : evidence for a non-A, non-B hepatitis virus aetiology", Lancet, 2, pp.876~879 (1980)
  7. Khuroo MS., "Study of an epidemic of non-A, non-B hepatitis. Possibility of another human hepatitis virus distinct from post-transfusion non-A, non-B type", Am. J. Med, 68, pp.818~824 (1980)
  8. Reyes GR, "Isolation o f a cDNA f rom the virus responsible for enterically transmitted non-A, non-B hepatitis", Science, 247, pp.1335~1339 (1990)
  9. Jilani N, "Hepatitis E virus i nf ection and fulminant hepatic failure during pregnancy", J. Gastroenterol. Hepatol, 22, pp.676~682 (2007)
  10. Navaneethan U, "Hepatitis E and pregnancy: understanding the pathogenesis", Liver Int, 28, pp.1190~1199 (2008)
  11. Theofilopoulos AN, "Type I interferons (alpha", beta) in immunity and autoimmunity, 2005, pp.23, 307~336
  12. Zitvogel L, "Type I interferons in anticancer immunity", Nat. Rev. Immunol, 15, pp.405~414 (2015)
  13. Nari Kim, "Multilayered regulations of RIG-I in the anti-viral signaling pathway", The Journal of Microbiology, 54(9), pp.583~587 (2016), 한국미생물학회[DOI]
  14. Megan E. Schmidt, "Modulation of the host immune response by respiratory syncytial virus proteins", The Journal of Microbiology, 55(3), pp.161~171 (2017), 한국미생물학회[DOI]
  15. Draenert R, "Virus immune evasion:new mechanism and implications in disease outcome", Adv. Virol, 2012, pp.490549~ (2012)
  16. Senba M, "Mechanisms of virus immune evasion lead to development from chronic inflammation to cancer formation associated with human papillomavirus infection", Oncol Rev, 6(2), pp.e17~ (2012)
  17. Md Bashir Uddin, "Inhibitory effects of bee venom and its components against viruses in vitro and in vivo", The Journal of Microbiology, 54(12), pp.853~866 (2016), 한국미생물학회[DOI]
  18. 강상민, "Hepatitis E Virus Methyltransferase Inhibits Type I Interferon Induction by Targeting RIG-I", Journal of Microbiology and Biotechnology, 28(9), pp.1554~1562 (2018), 한국미생물·생명공학회
  19. Nan Y, "Hepatitis E virus inhibits type I interferon induction by ORF1 products", J. Virol, 88, pp.11924~11932 (2014)
  20. Surjit M, "The ORF2 glycoprotein of hepatitis E virus inhibits cellular NF-kappaB activity by blocking ubiquitination mediated proteasomal degradation of IkappaBalpha in human hepatoma cells", BMC Biochem, 13, pp.7~ (2012)
  21. He M, "The ORF3 protein of genotype 1 Hepatitis E virus suppresses TLR3-induced NF-kappaB signaling via TRADD and RIP1", Sci. Rep, 6, pp.27597~ (2016)
  22. 최슬기, "Recombinant Adeno-Associated Virus Expressing Truncated IK Cytokine Diminishes the Symptoms of Inflammatory Arthritis", Journal of Microbiology and Biotechnology, 27(10), pp.1892~1895 (2017), 한국미생물·생명공학회
  23. Faysal Bin Hamid, "Characterization of Prototype Foamy Virus Infectivity in Transportin 3 Knockdown Human 293t Cell Line", Journal of Microbiology and Biotechnology, 27(2), pp.380~387 (2017), 한국미생물·생명공학회[DOI]
  24. 이진무, "A Novel Chemical Compound for Inhibition of SARS Coronavirus Helicase", Journal of Microbiology and Biotechnology, 27(11), pp.2070~2073 (2017), 한국미생물·생명공학회
  25. Xin Liu, "VSV-G Viral Envelope Glycoprotein Prepared from Pichia pastoris Enhances Transfection of DNA into Animal Cells", Journal of Microbiology and Biotechnology, 27(6), pp.1098~1105 (2017), 한국미생물·생명공학회[DOI]
  26. Ahn HS, "Adverse fetal outcomes in pregnant rabbits experimentally infected with rabbit hepatitis E virus", Virology, 512, pp.187~193 (2017)
  27. Cho M, "OX40 and 4-1BB downregulate Kaposi’s sarcoma-associated herpesvirus replication in lymphatic endothelial cells, but 4-1BB and not OX40 inhibits viral replication in B-cells", J. Gen. Virol, 96, pp.3635~3645 (2015)
  28. Fu B, "Activation of p90 ribosomal S6 kinases by ORF45 of Kaposi’s sarcoma-associated herpesvirus is critical for optimal production of infectious viruses", J. Virol, 89, pp.195~207 (2015)
  29. Gillen J, "A survey of the interactome of Kaposi’s sarcoma-associated herpesvirus ORF45 revealed its binding to viral ORF33 and cellular USP7, resulting in stabilization of ORF33 that is required for production of progeny viruses", J. Virol, 89, pp.4918~4931 (2015)
  30. Ha S, "Infection models of human norovirus: challenges and recent progress", Arch. Virol, 161, pp.779~788 (2016)
  31. Kang HS, "Transgenic expression of non-structural genes of Theiler’s virus suppresses initial viral replication and pathogenesis of demyelination", J. Neuroinflammation, 13, pp.133~ (2016)
  32. Lee M, "Detection of viable murine norovirus using the plaque assay and propidium-monoazide-combined real-time reverse transcription-polymerase chain reaction", J. Virol. Methods, 221, pp.57~61 (2015)
  33. Karpe YA, "Deubiquitination activity associated with hepatitis E virus putative papain-like cysteine protease", J. Gen. Virol, 92, pp.2088~2092 (2011)
  34. Oshiumi H, "A distinct role of Riplet-mediated K63-Linked polyubiquitination of the RIG-I repressor domain in human antiviral innate immune responses", PLoS Pathog, 9, pp.e1003533~ (2013)
  35. Akira S, "Pathogen recognition and innate immunity", Cell, 124, pp.783~801 (2006)
  36. 강상민, "Primary lymphocyte infection models for KSHV and its putative tumorigenesis mechanisms in B cell lymphomas", The Journal of Microbiology, 55(5), pp.319~329 (2017), 한국미생물학회
  37. Medzhitov R, "Recognition of microorganisms and activation of the immune response", Nature, 449, pp.819~826 (2007)
  38. 이병훈, "Coptidis Rhizoma extract inhibits replication of respiratory syncytial virus in vitro and in vivo by inducing antiviral state", The Journal of Microbiology, 55(6), pp.488~498 (2017), 한국미생물학회
  39. Rak-Kyun Seong, "MDA7", IL-24 is an anti-viral factor that inhibits influenza virus replication, 2016, pp.54(10), 695~700[DOI]
  40. Hsin-Chih Lai, "The assessment of host and bacterial proteins in sputum from active pulmonary tuberculosis", The Journal of Microbiology, 54(11), pp.761~767 (2016), 한국미생물학회[DOI]
  41. Prasanna Weeratunga, "Interferon-mediated antiviral activities of Angelica tenuissima Nakai and its active components", The Journal of Microbiology, 54(1), pp.57~70 (2016), 한국미생물학회[DOI]
  42. Prasanna Weeratunga, "Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo", The Journal of Microbiology, 55(11), pp.909~917 (2017), 한국미생물학회[DOI]
  43. Loo YM, "Distinct RIG-I and MDA5signaling by RNA viruses in innate immunity", J. Virol, 82, pp.335~345 (2008)
  44. Takeuchi O, "Pattern recognition receptors and inflammation", Cell, 140, pp.805~820 (2010)
  45. Kawai T, "IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction", Nat. Immunol, 6, pp.981~988 (2005)
  46. Meylan E, "Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus", Nature, 437, pp.1167~1172 (2005)
  47. Seth RB, "Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3", Cell, 122, pp.669~682 (2005)
  48. Grandvaux N, "Transcriptional profiling of interferon regulatory factor 3 target genes:direct involvement in the regulation of interferon-stimulated genes", J. Virol, 76, pp.5532~5539 (2002)
  49. gene induction by the interferon regulatory factor family of transcription factors, "Immunity", 2006, 349~360
  50. Liu S, "Phosphorylation of innate immune adaptor proteins MAVS, STING, and TRIF induces IRF3 activation", Science, 347, pp.aaa2630~ (2015)

관련 논문AI추천

Dose-Dependent Inhibition of Melanoma Differentiation-Associated Gene 5-Mediated Activation of Type I Interferon Responses by Methyltransferase of Hepatitis E Virus강상민, 명진종한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제7호Hepatitis E Virus Methyltransferase Inhibits Type I Interferon Induction by Targeting RIG-I강상민, 권요셉, 김성준, 노성운, 명진종, 박미경, 최인수, 최종선, 최창순, 한귀남한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2018 · 제28권 제9호Middle East Respiratory Syndrome Coronavirus-Encoded ORF8b Inhibits RIG-I-Like Receptors by a Differential Mechanism김성준, 명진종, 이정윤한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제12호Regulation of antiviral innate immune signaling and viral evasion following viral genome sensing이종수, Chathuranga Kiramage, Dodantenna Niranjan, Weerawardhana Asela생화학분자생물학회 · Experimental and Molecular Medicine2021 · 제53권 Middle East Respiratory Syndrome Coronavirus-Encoded Accessory Proteins Impair MDA5-and TBK1-Mediated Activation of NF-κB명진종, 배소정, 이정윤한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제8호Zika Virus Proteins NS2A and NS4A Are Major Antagonists that Reduce IFN-β Promoter Activity Induced by the MDA5/RIG-I Signaling Pathway김성준, 명진종, 이정윤, Nguyen Thi Thuy Ngan한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2019 · 제29권 제10호

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