A Role of YlBud8 in the Regulation of Cell Separation in the Yeast Yarrowia lipolytica
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The spatial landmark protein Bud8 plays a crucial role in bipolar budding in the budding yeast Saccharomyces cerevisiae. The unconventional yeast Yarrowia lipolytica can also bud in a bipolar pattern, but is evolutionarily distant from S. cerevisiae. It encodes the protein YALI0F12738p, which shares the highest amino acid sequence homology with S. cerevisiae Bud8, sharing a conserved transmembrane domain at the C-terminus. Therefore, we named it YlBud8. Deletion of YlBud8 in Y. lipolytica causes cellular separation defects, resulting in budded cells remaining linked with one another as cell chains or multiple buds from a single cell, which suggests that YlBud8 may play an important role in cell separation, which is distinct from the function of Bud8 in S. cerevisiae. We also show that the YlBud8-GFP fusion protein is located at the cell membrane and enriched in the bud cortex, which would be consistent with a role in the regulation of cell separation. The coiled-coil domain at the Nterminus of YlBud8 is important to the correct localization and function of YlBud8, as truncated proteins that do not contain the coiled-coil domain cannot rescue the defects observed in Ylbud8Δ. This finding suggests that a new signaling pathway controlled by YlBud8 via regulation of cell separation may exist in Y. lipolytica.
Abstract
The spatial landmark protein Bud8 plays a crucial role in bipolar budding in the budding yeast Saccharomyces cerevisiae. The unconventional yeast Yarrowia lipolytica can also bud in a bipolar pattern, but is evolutionarily distant from S. cerevisiae. It encodes the protein YALI0F12738p, which shares the highest amino acid sequence homology with S. cerevisiae Bud8, sharing a conserved transmembrane domain at the C-terminus. Therefore, we named it YlBud8. Deletion of YlBud8 in Y. lipolytica causes cellular separation defects, resulting in budded cells remaining linked with one another as cell chains or multiple buds from a single cell, which suggests that YlBud8 may play an important role in cell separation, which is distinct from the function of Bud8 in S. cerevisiae. We also show that the YlBud8-GFP fusion protein is located at the cell membrane and enriched in the bud cortex, which would be consistent with a role in the regulation of cell separation. The coiled-coil domain at the Nterminus of YlBud8 is important to the correct localization and function of YlBud8, as truncated proteins that do not contain the coiled-coil domain cannot rescue the defects observed in Ylbud8Δ. This finding suggests that a new signaling pathway controlled by YlBud8 via regulation of cell separation may exist in Y. lipolytica.
참고문헌 (32)
- Martin SG, "Cell polarity : a new mod(e)of anchoring", Curr. Biol, 13, pp.R711~R713 (2003)
- Drubin DG, "Origins of cell polarity", Cell, 84, pp.335~344 (1996)
- Schenkman LR, "The role of cell cycle regulated expression in the localization of spatial landmark proteins in yeast", J. Cell Biol, 156, pp.829~841 (2002)
- Bi E, "Cell polarization and cytokinesis in budding yeast", Genetics, 191, pp.347~387 (2012)
- Pollard TD, "Mechanics of cytokinesis in eukaryotes", Curr. Opin. Cell Biol, 22, pp.50~56 (2010)
- Barr FA, "Cytokinesis : placing and making the final cut", Cell, 131, pp.847~860 (2007)
- Chant J, "Cell polarity in yeast", Annu. Rev. Cell Dev. Biol, 15, pp.365~391 (1999)
- Li N, "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae", Mol. Bio. Cell, 12, pp.2147~2170 (2001)
- Park HO, "Central roles of small GTPases in the development of cell polarity in yeast and beyond", Microbiol. Mol. Biol. Rev, 71, pp.48~96 (2007)
- Park HO, "Two active states of the Ras-related Bud1", Rsr1 protein bind to different effectors to determine yeast cell polarity, 1997, pp.94, 4463~4468
- Kang PJ, "The Rsr1", Bud1 GTPase interacts with itself and the Cdc42 GTPase during bud-site selection and polarity establishment in budding yeast, 2010, pp.21, 3007~3016
- Lee ME, "Regulation of Cdc42 polarization by the Rsr1 GTPase and Rga1, a Cdc42 GTPase-activating protein, in budding yeast", J. Cell Sci, 128, pp.2106~2117 (2015)
- Basu S, "Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise", Proc. Natl. Acad. Sci. USA, 113, pp.2019~2028 (2016)
- Herrero AB, "Candida albicans and Yarrowia lipolytica as alternative models for analysing budding patterns and germ tube formation in dimorphic fungi", Microbiology, 145, pp.2727~2737 (1999)
- Gimeno CJ, "Unipolar cell divisions in the yeast S, cerevisiae lead to filamentous growth: regulation by starvation and RAS", Cell, 68, pp.1077~1090 (1992)
- Veses VN, "Pseudohypha budding patetrns of Candida albicans", Med. Mycol, 47, pp.268~275 (2008)
- Nicaud JM, "Yarrowia lipolytica", Yeast, 29, pp.409~418 (2012)
- Kang PJ, "Interactions among Rax1p, Rax2p, Bud8p, and Bud9p in marking cortical sites for bipolar bud-site selection in yeast", Mol. Biol. Cell, 15, pp.5145~5157 (2004)
- Kato Y, "Cell polarity in Saccharomyces cerevisiae depends on proper localization of the Bud9 landmark protein by the EKC", KEOPS complex, 2011, pp.188, 871~882
- Harkins HA, "Bud8 and Bud9, proteins that may mark the sites for bipolar budding in yeast", Mol. Bio. Cell, 12, pp.2497~2518 (2001)
- Li YQ, "A role for the Rap GTPase YlRsr1 in cellular morphogenesis and the involvement of YlRsr1 and the Ras GTPase YlRas2 in bud site selection in the dimorphic yeast Yarrowia lipolytica", Eukaryot. Cell, 13, pp.580~590 (2014)
- Richard M, "Tagging morphogenetic genes by insertional mutagenesis in the yeast Yarrowia lipolytica", J. Bacteriol, 183, pp.3098~3107 (2001)
- Zhao XF, "The TEA", ATTS transcription factor YlTec1p represses the yeast-hypha transition in the dimorphic yeast Yarrowia lipolytica, 2013, pp.13, 50~61
- Li M, "Roles of the three Ras proteins in the regulation of dimorphic transition in the yeast Yarrowia lipolytica", FEMS Yeast Res, 14, pp.451~463 (2014)
- Lupas A, "Coiled coils : new structures and new functions", Trends Biochem. Sci, 21, pp.375~382 (1996)
- Taheri N, "Asymmetrically localized Bud8p and Bud9p proteins control yeast cell polarity and development", EMBO J, 19, pp.6686~6696 (2000)
- Krappmann AB, "Distinct domains of yeast cortical tag proteins Bud8p and Bud9p confer polar localization and functionality", Mol. Biol. Cell, 18, pp.3323~3339 (2007)
- Movahed E, "Genome-wide transcription study of Cryptococcus neoformans H99 clinical strain versus environmental strains", PLoS One, 10, pp.e0137457~ (2015)
- Liu J, "Comparing function and structure between entire proteomes", Protein Sci, 10, pp.1970~1979 (2001)
- Surkont J, "Evolutionary patterns in coiled-coils", Genome Biol. Evol, 7, pp.545~556 (2015)
- Zhang H, "A computationally guided protein-interaction screen uncovers coiled-coil interactions involved in vesicular trafficking", J. Mol. Biol, 392, pp.228~241 (2009)
- Munro S, "The golgin coiled-coil proteins of the Golgi apparatus", Cold Spring Harb. Perspect. Biol, 3, pp.1~14 (2011)