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Zinc Ions Affect Siderophore Production by Fungi Isolated from the Panax ginseng Rhizosphere

주진호|칼리드후세인
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2019.01|Journal of Microbiology and Biotechnology|제29권 제1호|pp. 105-113|DOI 10.4014/jmb.1712.12026

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키워드

Zn2+siderophoreRoot-associated fungiPenicillium commune
주제분류생물학

초록

Although siderophore compounds are mainly biosynthesized as a response to iron deficiency in the environment, they also bind with other metals. A few studies have been conducted on the impact of heavy metals on the siderophore-mediated iron uptake by microbiome. Here, we investigated siderophore production by a variety of rhizosphere fungi under different concentrations of Zn2+ ion. These strains were specifically isolated from the rhizosphere of Panax ginseng (Korean ginseng). The siderophore production of isolated fungi was investigated with chrome azurol S (CAS) assay liquid media amended with different concentrations of Zn2+ (50 to 250 μg/ml). The percentage of siderophore units was quantified using the ultra-violet (UV) irradiation method. The results indicated that high concentrations of Zn2+ ion increase the production of siderophore in iron-limited cultures. Maximum siderophore production by the fungal strains was detected at Zn2+ ion concentration of 150 μg/ml except for Mortierella sp., which had the highest siderophore production at 200 μg/ml. One potent siderophoreproducing strain (Penicillium sp. JJHO) was strongly influenced by the presence of Zn2+ ions and showed high identity to P. commune (100% using 18S-rRNA sequencing). The purified siderophores of the Penicillium sp. JJHO strain were chemically identified using UV, Fouriertransform infrared spectroscopy (FTIR), and matrix-assisted laser desorption/ionization timeof- flight mass spectrometer (MALDI-TOF-MS) spectra.

Abstract

Although siderophore compounds are mainly biosynthesized as a response to iron deficiency in the environment, they also bind with other metals. A few studies have been conducted on the impact of heavy metals on the siderophore-mediated iron uptake by microbiome. Here, we investigated siderophore production by a variety of rhizosphere fungi under different concentrations of Zn2+ ion. These strains were specifically isolated from the rhizosphere of Panax ginseng (Korean ginseng). The siderophore production of isolated fungi was investigated with chrome azurol S (CAS) assay liquid media amended with different concentrations of Zn2+ (50 to 250 μg/ml). The percentage of siderophore units was quantified using the ultra-violet (UV) irradiation method. The results indicated that high concentrations of Zn2+ ion increase the production of siderophore in iron-limited cultures. Maximum siderophore production by the fungal strains was detected at Zn2+ ion concentration of 150 μg/ml except for Mortierella sp., which had the highest siderophore production at 200 μg/ml. One potent siderophoreproducing strain (Penicillium sp. JJHO) was strongly influenced by the presence of Zn2+ ions and showed high identity to P. commune (100% using 18S-rRNA sequencing). The purified siderophores of the Penicillium sp. JJHO strain were chemically identified using UV, Fouriertransform infrared spectroscopy (FTIR), and matrix-assisted laser desorption/ionization timeof- flight mass spectrometer (MALDI-TOF-MS) spectra.

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