Lactobacillus sakei S1 Improves Colitis Induced by 2,4,6-Trinitrobenzene Sulfonic Acid by the Inhibition of NF-κB Signaling in Mice
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Lactobacillus sakei S1 strongly inhibits the expression of interleukin (IL)-6 and IL-1β in lipopolysaccharide-induced peritoneal macrophages by a mechanism for which lactic acid bacteria from kimchi that inhibit tumor necrosis factor-alpha (TNF-α) were isolated. Therefore, we further evaluated the protective effect of this strain on the colitis mouse model induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). TNBS significantly elevated myeloperoxidase (MPO) expression, macroscopic scores, and colon shortening. Oral L. sakei S1 administration resulted in reduction of TNBS-induced loss in body weight, colon shortening, MPO activity, expression of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB). L. sakei S1 inhibited the expression of inflammatory cytokines IL-1β, IL-6 and TNF-α, induced by TNBS, but enhanced IL-10 expression. L. sakei S1 showed resistance to artificial digestive juices and adherence to intestinal epithelial Caco-2 cells. Thus, L. sakei S1 may inhibit the NF-κB pathway and be used in functional food to treat colitis.
Abstract
Lactobacillus sakei S1 strongly inhibits the expression of interleukin (IL)-6 and IL-1β in lipopolysaccharide-induced peritoneal macrophages by a mechanism for which lactic acid bacteria from kimchi that inhibit tumor necrosis factor-alpha (TNF-α) were isolated. Therefore, we further evaluated the protective effect of this strain on the colitis mouse model induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). TNBS significantly elevated myeloperoxidase (MPO) expression, macroscopic scores, and colon shortening. Oral L. sakei S1 administration resulted in reduction of TNBS-induced loss in body weight, colon shortening, MPO activity, expression of cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and nuclear factor-kappa B (NF-κB). L. sakei S1 inhibited the expression of inflammatory cytokines IL-1β, IL-6 and TNF-α, induced by TNBS, but enhanced IL-10 expression. L. sakei S1 showed resistance to artificial digestive juices and adherence to intestinal epithelial Caco-2 cells. Thus, L. sakei S1 may inhibit the NF-κB pathway and be used in functional food to treat colitis.
참고문헌 (31)
- Neuman MG, "Inflammatory bowel disease : role of diet, microbiota, life style", Trans. Res, 160, pp.29~44 (2012)
- Shanahan F, "Gut flora in gastrointestinal disease", Eur. J. Surg. Suppl, 587, pp.47~52 (2002)
- Jang SE, "Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages", Int. Immunopharmacol, 21, pp.186~192 (2014)
- Chandran P, "Inflammatory bowel disease : dysfunction of GALT and gut bacterial flora(I)", Surgeon, 1, pp.63~75 (2003)
- Aderem A, "Toll-like receptors in the induction of the innate immune response", Nature, 406, pp.782~787 (2000)
- Min SW, "Anti-inflammatory effects of black rice, cyanidin-3-O-β-D-glycoside, and its metabolites, cyanidin and protocatechuic acid", Int. Immunopharmacol, 10, pp.959~966 (2010)
- Hutchison S, "Tumour necrosis factoralpha blockade suppresses murine allergic airways inflammation", Clin. Exp. Immunol, 151, pp.114~122 (2008)
- Lee IA, "Soyasaponin Ab ameliorates colitis by inhibiting the binding of lipopolysaccharide(LPS)to Toll-like receptor(TLR)4 on macrophages", J. Agric. Food Chem, 59, pp.13165~13172 (2011)
- Collins MP, "Probiotics, prebiotics, and synbiotics : approaches for modulating the microbial ecology of the gut", Am. J. Clin. Nutr, 69, pp.1052S~1057S (1999)
- Aggarwal J, "Probiotics and their effects on metabolic diseases : an update", J. Clin. Diagn. Res, 7, pp.173~177 (2013)
- Romeo J, "Immunomodulatory effect of fibres, probiotics and synbiotics in different life-stages", Nutr. Hosp, 25, pp.341~349 (2010)
- Peran L, "A comparative study of the preventative effects exerted by two probiotics, Lactobacillus reuteri and Lactobacillus fermentum, in the trinitrobenzenesulfonic acid model of rat colitis", Br. J. Nutr., 97, pp.96~103 (2007)
- Okada Y, "Novel probiotics isolated from a Japanese traditional fermented food, Funazushi, attenuates DSS-induced colitis by increasing the induction of high integrin αv", β8-expressing dendritic cells, 2018, pp.53, 407~418
- Bellavia M, "Lactobacillus casei and Bifidobacterium lactis supplementation reduces tissue damage of intestinal mucosa and liver after 2, 4, 6-trinitrobenzenesulfonic acid treatment in mice", J. Biol. Regul. Homeost. Agents, 28, pp.251~261 (2014)
- Jang SE, "Simultaneous amelioratation of colitis and liver injury in mice by Bifidobacterium longum LC67 and Lactobacillus plantarum LC27", Sci. Rep, 8, pp.7500~ (2018)
- Se-Eun Jang, "Adhesion Activity of Lactobacillus plantarum PM 008 Isolated from Kimchi on the Intestine of Mice", Journal of Bacteriology and Virology, 41(2), pp.83~90 (2011), 대한미생물학회
- Kos B, "Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92", J. Appl. Microbiol, 94, pp.981~987 (2003)
- Lim SM, "A mixture of the probiotic strains Bifidobacterium longum CH57and Lactobacillus brevis CH23 ameliorates colitis in mice by inhibiting macrophage activation and restoring the Th17", Treg balance, 2016, pp.27, 295~309
- Kwon KH, "Interleukin-1beta targets interleukin-6 in progressing dextran sulfate sodium-induced experimental colitis", Biochem. Biophys. Res. Commun., 337, pp.647~654 (2005)
- Ljungh A, "Lactic acid bacteria as probiotics", Curr. Issues Intestinal Microbiol, 7, pp.73~90 (2006)
- Lee KW, "Probiotic properties of Pediococcus strains isolated from Jeotgals, salted and fermented Korean sea-food", Ananerobe, 28, pp.199~206 (2014)
- Saarela M, "Probiotic bacteria : safety, functional and technological properties", J. Biotechnol, 84, pp.197~215 (2000)
- Fiorucci S, "Importance of innate immunity and collagen binding integrin alpha1beta1 in TNBS-induced colitis", Immunity, 17, pp.769~780 (2002)
- Neurath MF, "Predominant pathogenic role of tumor necrosis factor in experimental colitis in mice", Eur. J. Immunol, 27, pp.1743~1750 (1997)
- Fiorucci S, "Proteinase-activated receptor(PAR)-2 is an anti-inflammatory signal for colonic lamina propria lymphocytes in a mouse model of colitis", Proc. Natl. Acad. Sci. USA, 98, pp.13936~13941 (2001)
- Iyer SS, "Role of interleukin 10 transcriptional regulation in inflammation and autoimmune disease", Crit. Rev. Immunol, 32, pp.23~63 (2012)
- Barens PJ, "Nuclear factor-kappaB : a pivotal transcription factor in chronic inflammatory diseases", N. Engl. J. Med, 336, pp.1066~1071 (1997)
- Collins T, "Transcriptional regulation of endothelial cell adhesion molecules : NF-κB and cytokine inducible enhancers", FASEB. J, 9, pp.899~909 (1995)
- Yu YR, "Clinical presentation of Crohn’s, ulcerative colitis, and indeterminate colitis : symptoms, extraintestinal manifestations, and disease phenotypes", Semin. Pediatr. Surg, 26, pp.349~355 (2017)
- Chami B, "Myeloperoxidase in the inflamed colon : a novel target for treating inflammatory bowel disease", Arch. Biochem. Biophys, 645, pp.61~71 (2018)
- Jang SE, "Penta-O-galloyl-β-D-glucose ameliorates inflammation by inhibiting MyD88", NF-κB and MyD88, Br. J. Pharmacol, pp.2013, 170[DOI]