LINC00174 Facilitates Proliferation and Migration of Colorectal Cancer Cells via MiR-3127-5p/ E2F7 Axis
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The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR- 3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of ECadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.
Abstract
The literature indicates that LINC00174 promotes the growth of colorectal cancer (CRC) cells, but its research needs to be enriched. We tried to explore the function and mechanism of LINC00174 in CRC cell proliferation and migration. Bioinformatics analysis predicted the binding relationship and expressions of lncRNA, miRNA and mRNA. Clinical study analyzes the relationship between LINC00174 and clinical data characteristics of CRC patients. The expressions of LINC00174, miR- 3127-5p and E2F7 were verified by RT-qPCR, and the combination of the two was verified by dual luciferase analysis and RNA immunoprecipitation as needed. Western blot was used to detect the expression of EMT-related protein and E2F7 protein. Functional experiments were used to evaluate the function of the target gene on CRC cells. LINC00174 was up-regulated in CRC clinical samples and cells and was related to the clinical characteristics of CRC patients. High-expression of LINC00174, contrary to the effect of siLINC00174, promoted cell viability, proliferation, migration and invasion, up-regulated the expressions of N-Cadherin, Vimentin, E2F7, and inhibited the expression of ECadherin. MiR-3127-5p was one of the targeted miRNAs of LINC00174 and was down-regulated in CRC samples. In addition, miR-3127-5p mimic partially reversed the malignant phenotype of CRC cells induced by LINC00174. Besides, E2F7 was a target gene of miR-3127-5p, and LINC00174 repressed miR-3127-5p to regulate E2F7. Our research reveals that LINC00174 affected the biological characteristics of CRC cells through regulated miR-3127-5p/ E2F7 axis.
참고문헌 (36)
- Patel SG, "Colorectal cancer in the young", Curr. Gastroenterol. Rep, 20, pp.15~ (2018)
- Cao H, "Epithelial-mesenchymal transition in colorectal cancer metastasis : a system review", Pathol. Res. Pract, 211, pp.557~569 (2015)
- Modest DP, "Sartore-Bianchi A. Treatment sequencing in metastatic colorectal cancer", Eur. J. Cancer, 109, pp.70~83 (2019)
- Marmol I, "Colorectal carcinoma : a general overview and future perspectives in colorectal cancer", Int. J. Mol. Sci, 18, pp.197~ (2017)
- Yiu AJ, "Biomarkers in colorectal cancer", Anticancer Res, 36, pp.1093~1102 (2016)
- Li H, "Roles of long noncoding RNAs in colorectal cancer metastasis", Oncotarget, 8, pp.39859~39876 (2017)
- Yin DD, "Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer", Tumour Biol, 36, pp.4851~4859 (2015)
- Liang WC, "The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer", Oncotarget, 6, pp.22513~22525 (2015)
- Ding J, "Long non-coding RNA Loc554202 induces apoptosis in colorectal cancer cells via the caspase cleavage cascades", J. Exp. Clin. Cancer Res, 34, pp.100~ (2015)
- Shi J, "Long non-coding RNA LINC00174 promotes glycolysis and tumor progression by regulating miR-152-3p", SLC2A1 axis in glioma, 2019, pp.38, 395~
- Li B, "LINC00174 down-regulation decreases chemoresistance to temozolomide in human glioma cells by regulating miR-138-5p", SOX9 axis, 2020, pp.33, 159~174
- Wang Z, "LINC00174 is a favorable prognostic biomarker in glioblastoma via promoting proliferative phenotype", Cancer Biomark, 28, pp.421~427 (2020)
- Zhao JT, "LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320", S100A10 axis, 2020, pp.38, 859~869
- Tito C, "LINC00174 is a novel prognostic factor in thymic epithelial tumors involved in cell migration and lipid metabolism", Cell Death Dis, 11, pp.959~ (2020)
- Liu J, "Promotion of BZW2 by LINC00174 through miR-4500 inhibition enhances proliferation and apoptosis evasion in laryngeal papilloma", Cancer Cell Int, 20, pp.471~ (2020)
- Shen Y, "STAT1-mediated upregulation of lncRNA LINC00174 functions a ceRNA for miR-1910-3p to facilitate colorectal carcinoma progression through regulation of TAZ", Gene, 666, pp.64~71 (2018)
- Jiaming Zhu, "Regulation Mechanism of Long Noncoding RNAs in Colon Cancer Development and Progression", Yonsei Medical Journal, 60(4), pp.319~325 (2019), 연세대학교의과대학[DOI]
- Xie X, "Long non-coding RNAs in colorectal cancer", Oncotarget, 7, pp.5226~5239 (2016)
- Chen DL, "Long non-coding RNA UICLM promotes colorectal cancer liver metastasis by acting as a ceRNA for microRNA-215 to regulate ZEB2 expression", Theranostics, 7, pp.4836~4849 (2017)
- Miao C, "A brief review : the therapeutic potential of bone marrow mesenchymal stem cells in myocardial infarction", Stem Cell Res. Ther, 8, pp.242~ (2017)
- Dayong Ding, "LncRNA H19", miR-29b-3p, Molecules and Cells, pp.2018, 41(5)[DOI]
- Yang Y, "Downregulation of miR-3127-5p promotes epithelial-mesenchymal transition via FZD4 regulation of Wnt", beta-catenin signaling in non-small-cell lung cancer, 2018, pp.57, 842~853
- Pan B, "Long noncoding RNA gastric cancer-associated transcript 3 plays oncogenic roles in glioma through sponging miR-3127-5p", J. Cell Physiol, 234, pp.8825~8833 (2019)
- Ding B, "Whole-transcriptome analysis reveals a potential hsa_circ_0001955", hsa_circ_0000977-mediated miRNA-mRNA regulatory sub-network in colorectal cancer, 2020, pp.12, 5259~5279
- Wang W, "LncRNA MEG3 acts a biomarker and regulates cell functions by targeting ADAR1 in colorectal cancer", World J. Gastroenterol, 25, pp.3972~3984 (2019)
- Yang MH, "MALAT1 promotes colorectal cancer cell proliferation", migration, Biochim. Biophys. Acta, pp.2015, 1852[DOI]
- Xu Y, "The effects of lncRNA MALAT1 on proliferation, invasion and migration in colorectal cancer through regulating SOX9", Mol. Med, 24, pp.52~ (2018)
- Guo J, "Role of linc00174", miR-138-5p(miR-150-5p), Mol. Ther. Nucleic Acids, pp.2019, 18[DOI]
- Wang Z, "LINC00174 is a favorable prognostic biomarker in glioblastoma via promoting proliferative phenotype", Cancer Biomark, 28, pp.421~427 (2020)
- Zhao JT, "LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320", S100A10 axis, 2020, pp.38, 859~869
- Tang XJ, "Interactions among lncRNAs, miRNAs and mRNA in colorectal cancer", Biochimie, 163, pp.58~72 (2019)
- Liu W, "The protective role of all-transretinoic acid(ATRA)against colorectal cancer development is achieved via increasing miR-3666 expression and decreasing E2F7 expression", Biomed. Pharmacother, 104, pp.94~101 (2018)
- To KK, "MicroRNAs in the prognosis and therapy of colorectal cancer : from bench to bedside", World J. Gastroenterol, 24, pp.2949~2973 (2018)
- Zhou C, "MiR-185-3p mimic promotes the chemosensitivity of CRC cells via AQP5", Cancer Biol. Ther, 21, pp.790~798 (2020)
- Ma J, "LINC02163 promotes colorectal cancer progression via miR-511-3p", AKT3 axis, 2020, pp.48, 961~968
- Yu X, "SNHG1 represses the anti-cancer roles of baicalein in cervical cancer through regulating miR-3127-5p", FZD4, beta-catenin signaling, pp.Exp. Biol. Med, 2020[DOI]